PMID- 25259443
OWN - NLM
STAT- MEDLINE
DCOM- 20150824
LR  - 20211021
IS  - 1364-5528 (Electronic)
IS  - 0003-2654 (Print)
IS  - 0003-2654 (Linking)
VI  - 139
IP  - 22
DP  - 2014 Nov 21
TI  - Ultrasensitive carbohydrate-peptide SPR imaging microarray for diagnosing IgE 
      mediated peanut allergy.
PG  - 5728-33
LID - 10.1039/c4an01544d [doi]
AB  - Severity of peanut allergies is linked to allergen-specific immunoglobulin E 
      (IgE) antibodies in blood, but diagnostics from assays using glycoprotein 
      allergen mixtures may be inaccurate. Measuring IgEs specific to individual 
      peptide and carbohydrate epitopes of allergenic proteins is promising. We report 
      here the first immunoarray for IgEs utilizing both peptide and carbohydrate 
      epitopes. A surface plasmon resonance imaging (SPRi) microarray was equipped with 
      peptide and beta-xylosyl glycoside (BXG) epitopes from major peanut allergen 
      glycoprotein Arachis hypogaea h2 (Ara-h2). A monoclonal anti-IgE antibody was 
      included as positive control. IgEs were precaptured onto magnetic beads loaded 
      with polyclonal anti-IgE antibodies to enhance sensitivity and minimize 
      non-specific binding. As little as 0.1 attomole (0.5 pg mL(-1)) IgE was detected 
      from dilute serum in 45 min. IgEs binding to Ara-h2 peptide and BXG were 
      quantified in 10 muL of patient serum and correlated with standard ImmunoCAP 
      values.
FAU - Joshi, Amit A
AU  - Joshi AA
AD  - Department of Chemistry, University of Connecticut, U-3060, Storrs, CT 
      06269-3060, USA. James.Rusling@uconn.edu.
FAU - Peczuh, Mark W
AU  - Peczuh MW
FAU - Kumar, Challa V
AU  - Kumar CV
FAU - Rusling, James F
AU  - Rusling JF
LA  - eng
GR  - R01 EB014586/EB/NIBIB NIH HHS/United States
GR  - EB014586/EB/NIBIB NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Validation Study
PL  - England
TA  - Analyst
JT  - The Analyst
JID - 0372652
RN  - 0 (Carbohydrates)
RN  - 0 (Peptides)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
EIN - Analyst. 2014 Dec 21;139(24):6589
EIN - Analyst. 2014 Nov 7;139(21):5614
MH  - Carbohydrates/*analysis
MH  - Humans
MH  - Immunoglobulin E/*immunology
MH  - Limit of Detection
MH  - Peanut Hypersensitivity/*diagnosis/immunology
MH  - Peptides/*analysis
MH  - *Surface Plasmon Resonance
PMC - PMC4205568
MID - NIHMS631530
EDAT- 2014/09/27 06:00
MHDA- 2015/08/25 06:00
PMCR- 2015/11/21
CRDT- 2014/09/27 06:00
PHST- 2014/09/27 06:00 [entrez]
PHST- 2014/09/27 06:00 [pubmed]
PHST- 2015/08/25 06:00 [medline]
PHST- 2015/11/21 00:00 [pmc-release]
AID - 10.1039/c4an01544d [doi]
PST - ppublish
SO  - Analyst. 2014 Nov 21;139(22):5728-33. doi: 10.1039/c4an01544d.