PMID- 25259797
OWN - NLM
STAT- MEDLINE
DCOM- 20150610
LR  - 20211021
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 9
IP  - 9
DP  - 2014
TI  - HLA class I expression and its alteration by preoperative 
      hyperthermo-chemoradiotherapy in patients with rectal cancer.
PG  - e108122
LID - 10.1371/journal.pone.0108122 [doi]
LID - e108122
AB  - OBJECTIVE: Enhancing immunologic responses, including human leukocyte antigen 
      (HLA) class I expression on tumor cells and recognition and elimination of tumor 
      cells by tumor-specific cytotoxic T lymphocyte (CTL), is considered a novel 
      concept of radiotherapy. The present study examined patients who underwent 
      preoperative hyperthermo-chemoradiotherapy (HCRT) for locally advanced rectal 
      cancer to assess the correlation between HLA class I expression and clinical 
      outcome. MATERIALS AND METHODS: Seventy-eight patients with locally advanced 
      rectal adenocarcinoma who received preoperative HCRT were enrolled. The median 
      age of the patients was 64 years (range, 33-85 years) and 4, 18, and 56 patients 
      had clinical stage I, II and III disease, respectively. Formalin-fixed and 
      paraffin-embedded tissues excised before and after HCRT were subjected to 
      immunohistochemical analysis with an anti-HLA class I-A, B, C antibody. HLA class 
      I expression was graded according to tumor cell positivity. RESULTS: In pre-HCRT, 
      the number of specimens categorized as Grade 0 and 1 were 19 (24%) and 58 (74%), 
      respectively. Only 1 patient (1%) showed Grade 2 expression. However, 6 (8%), 27 
      (35%), 7 (9%), and 12 (15%) post-HCRT specimens were graded as Grade 0, 1, 2, and 
      3, respectively. There was a significant increase in HLA class I expression in 
      post-HCRT specimens (p<0.01). However, neither pre- nor post-HCRT HLA class I 
      expression affected overall survival and distant metastasis-free survival in 
      clinical stage III patients. Univariate analysis revealed that Post-HCRT HLA 
      class I expression showed a significant negative relationship with LC (p<0.05). 
      Nevertheless, multivariate analysis showed that there was no correlation between 
      HLA class I expression and clinical outcome. CONCLUSION: HCRT increased HLA class 
      I expression in rectal cancer patients. However, multivariate analysis failed to 
      show any correlation between the level of HLA class I expression and prognosis.
FAU - Sato, Hiro
AU  - Sato H
AD  - Department of Radiation Oncology, Gunma University Graduate School of Medicine, 
      Maebashi, Japan.
FAU - Suzuki, Yoshiyuki
AU  - Suzuki Y
AD  - Department of Radiation Oncology, Gunma University Graduate School of Medicine, 
      Maebashi, Japan.
FAU - Ide, Munenori
AU  - Ide M
AD  - Department of Human Pathology, Gunma University Graduate School of Medicine, 
      Maebashi, Japan.
FAU - Katoh, Toshihide
AU  - Katoh T
AD  - Department of General Surgical Science, Gunma University Graduate School of 
      Medicine, Maebashi, Japan.
FAU - Noda, Shin-Ei
AU  - Noda SE
AD  - Department of Radiation Oncology, Gunma University Graduate School of Medicine, 
      Maebashi, Japan.
FAU - Ando, Ken
AU  - Ando K
AD  - Department of Radiation Oncology, Gunma University Graduate School of Medicine, 
      Maebashi, Japan.
FAU - Oike, Takahiro
AU  - Oike T
AD  - Department of Radiation Oncology, Gunma University Graduate School of Medicine, 
      Maebashi, Japan.
FAU - Yoshimoto, Yuya
AU  - Yoshimoto Y
AD  - Department of Radiation Oncology, Gunma University Graduate School of Medicine, 
      Maebashi, Japan.
FAU - Okonogi, Noriyuki
AU  - Okonogi N
AD  - Department of Radiation Oncology, Gunma University Graduate School of Medicine, 
      Maebashi, Japan.
FAU - Mimura, Kousaku
AU  - Mimura K
AD  - Department of Surgery, National University of Singapore, Singapore, Singapore.
FAU - Asao, Takayuki
AU  - Asao T
AD  - Department of Oncology Clinical Development, Gunma University Graduate School of 
      Medicine, Maebashi, Japan.
FAU - Kuwano, Hiroyuki
AU  - Kuwano H
AD  - Department of General Surgical Science, Gunma University Graduate School of 
      Medicine, Maebashi, Japan.
FAU - Nakano, Takashi
AU  - Nakano T
AD  - Department of Radiation Oncology, Gunma University Graduate School of Medicine, 
      Maebashi, Japan.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140926
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Histocompatibility Antigens Class I)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - *Chemoradiotherapy
MH  - Combined Modality Therapy
MH  - Female
MH  - Gene Expression
MH  - Histocompatibility Antigens Class I/genetics/*immunology/metabolism
MH  - Humans
MH  - *Hyperthermia, Induced
MH  - Immunohistochemistry
MH  - Male
MH  - Middle Aged
MH  - Neoplasm Grading
MH  - Neoplasm Metastasis
MH  - Neoplasm Staging
MH  - Prognosis
MH  - Rectal Neoplasms/*immunology/mortality/pathology/*therapy
PMC - PMC4178123
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2014/09/27 06:00
MHDA- 2015/06/11 06:00
PMCR- 2014/09/26
CRDT- 2014/09/27 06:00
PHST- 2014/03/27 00:00 [received]
PHST- 2014/08/20 00:00 [accepted]
PHST- 2014/09/27 06:00 [entrez]
PHST- 2014/09/27 06:00 [pubmed]
PHST- 2015/06/11 06:00 [medline]
PHST- 2014/09/26 00:00 [pmc-release]
AID - PONE-D-14-13337 [pii]
AID - 10.1371/journal.pone.0108122 [doi]
PST - epublish
SO  - PLoS One. 2014 Sep 26;9(9):e108122. doi: 10.1371/journal.pone.0108122. 
      eCollection 2014.