PMID- 25260581 OWN - NLM STAT- MEDLINE DCOM- 20160108 LR - 20181202 IS - 1872-7573 (Electronic) IS - 0378-8741 (Linking) VI - 157 DP - 2014 Nov 18 TI - Abacopteris penangiana exerts testosterone-induced benign prostatic hyperplasia protective effect through regulating inflammatory responses, reducing oxidative stress and anti-proliferative. PG - 105-13 LID - S0378-8741(14)00679-5 [pii] LID - 10.1016/j.jep.2014.09.025 [doi] AB - ETHNOPHARMACOLOGICAL RELEVANCE: Abacopteris penangiana (Hook.) Ching (AP) is a member of parathelypteris glanduligera and used in folk medicine for the treatment of blood circulation and blood stasis, edema and inflammation as recorded in the ''Chinese Materia Medica''. AIM OF THE STUDY: The purpose of this study was to investigate the effects of total flavanol glycosides (TFA) from AP and its acid hydrolysate (AHT) on testosterone-induced benign prostatic hyperplasia (BPH) in rats by measuring the levels of inflammatory responses, oxidative stress and prostate cell proliferation. MATERIALS AND METHODS: BPH was induced in rats by subcutaneous injection of testosterone after castration. Seventy rats were divided into seven groups. After oral administration of AHT and TFA (100 or 200mg/kg/d) for 4 weeks, the prostate index (PI), 5a-reductase (5alpha-R) and dihydrotestosterone (DHT) were determined. Then the activities of superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase (CAT) were determined. In addition, the relative inflammatory factors, cyclooxygenase-2 (COX-2), tumor necrosis factor-alpha (TNF-alpha), interleukin 1beta (IL-1beta), interleukin 6 (IL-6), interleukin 8 (IL-8) and interleukin 17 (IL-17) were measured. Finally, the prostatic expression of nuclear transcription factor-kappaB (NF-kappaB) and phosphoinositide3-kinase (PI3K)/Akt were determined by immunohistochemistry. The prostatic expression of Bcl-2 was determined by western blot analysis. RESULTS: The results showed that AHT and TFA decreased serum DHT and 5alpha-R activities compared with model group, as well as the PI and histopathological examination findings. In addition, oral treatment of AHT and TFA can significantly increase the activities of SOD, GPx and CAT while the level of MDA was significantly decreased compared with the model group. Moreover, AHT and TFA remarkably decreased the levels of inflammatory cytokines in prostatic tissue. Further investigation demonstrated that AHT and TFA treatment down-regulated the protein expressions of p-Akt, NF-kappaB and Bcl-2. CONCLUSIONS: These results suggest that AHT and TFA have anti-BPH properties via anti-inflammatory, antioxidant and anti-proliferative effects. Hence, AP represents a potential herb for the treatment of BPH. CI - Copyright (c) 2014 Elsevier Ireland Ltd. All rights reserved. FAU - Yang, Xian AU - Yang X AD - Key Laboratory of Natural Medicinal Chemistry and Resource Evaluation of Hubei Province, College of Pharmacy, Tongji Medical Center, Huazhong University of Science and Technology, No. 13 Hangkong Road, Wuhan 430030, Hubei Province, China. FAU - Yuan, Liuliu AU - Yuan L AD - Key Laboratory of Natural Medicinal Chemistry and Resource Evaluation of Hubei Province, College of Pharmacy, Tongji Medical Center, Huazhong University of Science and Technology, No. 13 Hangkong Road, Wuhan 430030, Hubei Province, China. FAU - Xiong, Chaomei AU - Xiong C AD - Department of Pharmaceutical Analysis, Tongji Medical Center of Huazhong University of Science and Technology, Wuhan 430030, China. FAU - Yin, Chunping AU - Yin C AD - Key Laboratory of Natural Medicinal Chemistry and Resource Evaluation of Hubei Province, College of Pharmacy, Tongji Medical Center, Huazhong University of Science and Technology, No. 13 Hangkong Road, Wuhan 430030, Hubei Province, China. FAU - Ruan, Jinlan AU - Ruan J AD - Key Laboratory of Natural Medicinal Chemistry and Resource Evaluation of Hubei Province, College of Pharmacy, Tongji Medical Center, Huazhong University of Science and Technology, No. 13 Hangkong Road, Wuhan 430030, Hubei Province, China. Electronic address: jinlan8152@163.com. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20140923 PL - Ireland TA - J Ethnopharmacol JT - Journal of ethnopharmacology JID - 7903310 RN - 0 (Antioxidants) RN - 0 (Plant Extracts) RN - 3XMK78S47O (Testosterone) SB - IM MH - Animals MH - Antioxidants/administration & dosage/isolation & purification/pharmacology MH - Cell Proliferation/drug effects MH - Disease Models, Animal MH - Gene Expression Regulation/drug effects MH - Inflammation/drug therapy/pathology MH - Male MH - Medicine, Traditional MH - Oxidative Stress/*drug effects MH - Plant Extracts/administration & dosage/*pharmacology MH - Prostatic Hyperplasia/*drug therapy/pathology MH - Rats MH - Rats, Sprague-Dawley MH - Testosterone/toxicity MH - Tracheophyta/*chemistry OTO - NOTNLM OT - Abacopteris penangiana OT - Apoptosis OT - Benign prostatic hyperplasia OT - Inflammatory responses OT - Oxidative stress EDAT- 2014/09/28 06:00 MHDA- 2016/01/09 06:00 CRDT- 2014/09/28 06:00 PHST- 2014/03/29 00:00 [received] PHST- 2014/09/01 00:00 [revised] PHST- 2014/09/15 00:00 [accepted] PHST- 2014/09/28 06:00 [entrez] PHST- 2014/09/28 06:00 [pubmed] PHST- 2016/01/09 06:00 [medline] AID - S0378-8741(14)00679-5 [pii] AID - 10.1016/j.jep.2014.09.025 [doi] PST - ppublish SO - J Ethnopharmacol. 2014 Nov 18;157:105-13. doi: 10.1016/j.jep.2014.09.025. Epub 2014 Sep 23.