PMID- 25261229
OWN - NLM
STAT- MEDLINE
DCOM- 20150831
LR  - 20161125
IS  - 1532-2971 (Electronic)
IS  - 1090-0233 (Linking)
VI  - 202
IP  - 3
DP  - 2014 Dec
TI  - Engraftment of autologous bone marrow cells into the injured cranial cruciate 
      ligament in dogs.
PG  - 448-54
LID - S1090-0233(14)00368-2 [pii]
LID - 10.1016/j.tvjl.2014.08.031 [doi]
AB  - Current research indicates that exogenous stem cells may accelerate reparative 
      processes in joint disease but, no previous studies have evaluated whether bone 
      marrow cells (BMCs) target the injured cranial cruciate ligament (CCL) in dogs. 
      The objective of this study was to investigate engraftment of BMCs following 
      intra-articular injection in dogs with spontaneous CCL injury. Autologous 
      PKH26-labelled BMCs were injected into the stifle joint of eight client-owned 
      dogs with CCL rupture. The effects of PKH26 staining on cell viability and PKH26 
      fluorescence intensity were analysed in vitro using a MTT assay and flow 
      cytometry. Labelled BMCs in injured CCL tissue were identified using fluorescence 
      microscopy of biopsies harvested 3 and 13 days after intra-articular BMC 
      injection. The intensity of PKH26 fluorescence declines with cell division but 
      was still detectable after 16 days. Labelling with PKH26 had no detectable effect 
      on cell viability or proliferation. Only rare PKH26-positive cells were present 
      in biopsies of the injured CCL in 3/7 dogs and in synovial fluid in 1/7 dogs. No 
      differences in transforming growth factor-beta1, and interleukin-6 before and after 
      BMC treatment were found and no clinical complications were noted during a 1 year 
      follow-up period. In conclusion, BMCs were shown to engraft to the injured CCL in 
      dogs when injected into the articular cavity. Intra-articular application of 
      PKH26-labelled cultured mesenchymal stem cells is likely to result in higher 
      numbers of engrafted cells that can be tracked using this method in a clinical 
      setting.
CI  - Copyright (c) 2014 Elsevier Ltd. All rights reserved.
FAU - Linon, E
AU  - Linon E
AD  - Division of Small Animal Surgery and Orthopedics, Vetsuisse Faculty Bern, 
      Department of Clinical Veterinary Medicine, University of Bern, Langgassstrasse 
      128, 3012 Bern, Switzerland.
FAU - Spreng, D
AU  - Spreng D
AD  - Division of Small Animal Surgery and Orthopedics, Vetsuisse Faculty Bern, 
      Department of Clinical Veterinary Medicine, University of Bern, Langgassstrasse 
      128, 3012 Bern, Switzerland.
FAU - Rytz, U
AU  - Rytz U
AD  - Division of Small Animal Surgery and Orthopedics, Vetsuisse Faculty Bern, 
      Department of Clinical Veterinary Medicine, University of Bern, Langgassstrasse 
      128, 3012 Bern, Switzerland.
FAU - Forterre, S
AU  - Forterre S
AD  - Division of Small Animal Surgery and Orthopedics, Vetsuisse Faculty Bern, 
      Department of Clinical Veterinary Medicine, University of Bern, Langgassstrasse 
      128, 3012 Bern, Switzerland. Electronic address: 
      simone.forterre@vetsuisse.unibe.ch.
LA  - eng
PT  - Journal Article
DEP - 20140903
PL  - England
TA  - Vet J
JT  - Veterinary journal (London, England : 1997)
JID - 9706281
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Organic Chemicals)
RN  - 0 (PKH 26)
SB  - IM
MH  - Animals
MH  - *Anterior Cruciate Ligament Injuries
MH  - Bone Marrow Transplantation/*veterinary
MH  - Dog Diseases/*surgery
MH  - Dogs
MH  - Fluorescent Dyes/*adverse effects/therapeutic use
MH  - Injections, Intra-Articular/veterinary
MH  - Joint Diseases/surgery/*veterinary
MH  - Organic Chemicals/*adverse effects/therapeutic use
MH  - Rupture/therapy/veterinary
OTO - NOTNLM
OT  - Bone marrow cells
OT  - Cranial cruciate ligament
OT  - Dog
OT  - Mesenchymal stem cells, PKH26
OT  - Transplantation
EDAT- 2014/09/28 06:00
MHDA- 2015/09/01 06:00
CRDT- 2014/09/28 06:00
PHST- 2014/01/21 00:00 [received]
PHST- 2014/08/18 00:00 [revised]
PHST- 2014/08/27 00:00 [accepted]
PHST- 2014/09/28 06:00 [entrez]
PHST- 2014/09/28 06:00 [pubmed]
PHST- 2015/09/01 06:00 [medline]
AID - S1090-0233(14)00368-2 [pii]
AID - 10.1016/j.tvjl.2014.08.031 [doi]
PST - ppublish
SO  - Vet J. 2014 Dec;202(3):448-54. doi: 10.1016/j.tvjl.2014.08.031. Epub 2014 Sep 3.