PMID- 2526131
OWN - NLM
STAT- MEDLINE
DCOM- 19890821
LR  - 20220316
IS  - 0021-9525 (Print)
IS  - 1540-8140 (Electronic)
IS  - 0021-9525 (Linking)
VI  - 109
IP  - 1
DP  - 1989 Jul
TI  - Inhibition of endothelial cell movement by pericytes and smooth muscle cells: 
      activation of a latent transforming growth factor-beta 1-like molecule by plasmin 
      during co-culture.
PG  - 309-15
AB  - When a confluent monolayer of bovine aortic endothelial (BAE) cells is wounded 
      with a razor blade, endothelial cells (ECs) spontaneously move into the denuded 
      area. If bovine pericytes or smooth muscle cells (SMCs) are plated into the 
      denuded area at low density, they block the movement of the ECs. This effect is 
      dependent upon the number of cells plated into the wound area and contact between 
      ECs and the plated cells. Antibodies to transforming growth factor-beta 1 
      (TGF-beta 1) abrogate the inhibition of BAE cell movement by pericytes or SMCs. 
      TGF-beta 1, if added to wounded BAE cell monolayers, also inhibits cell movement. 
      When cultured separately, BAE cells, pericytes, and SMCs each produce an inactive 
      TGF-beta 1-like molecule which is activated in BAE cell-pericyte or BAE cell-SMC 
      co-cultures. The activation appears to be mediated by plasmin as the inhibitory 
      effect on cell movement in co-cultures of BAE cells and pericytes is blocked by 
      the inclusion of inhibitors of plasmin in the culture medium.
FAU - Sato, Y
AU  - Sato Y
AD  - Department of Cell Biology, New York University Medical Center, New York.
FAU - Rifkin, D B
AU  - Rifkin DB
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Cell Biol
JT  - The Journal of cell biology
JID - 0375356
RN  - 62031-54-3 (Fibroblast Growth Factors)
RN  - 76057-06-2 (Transforming Growth Factors)
RN  - EC 3.4.21.7 (Fibrinolysin)
SB  - IM
MH  - Animals
MH  - Cattle
MH  - Cell Communication
MH  - Cell Movement
MH  - Cells, Cultured
MH  - Endothelium, Vascular/*cytology
MH  - Fibrinolysin/*physiology
MH  - Fibroblast Growth Factors/physiology
MH  - In Vitro Techniques
MH  - Microcirculation/cytology
MH  - Muscle, Smooth, Vascular/*cytology
MH  - Transforming Growth Factors/*physiology
PMC - PMC2115489
EDAT- 1989/07/01 00:00
MHDA- 1989/07/01 00:01
PMCR- 1990/01/01
CRDT- 1989/07/01 00:00
PHST- 1989/07/01 00:00 [pubmed]
PHST- 1989/07/01 00:01 [medline]
PHST- 1989/07/01 00:00 [entrez]
PHST- 1990/01/01 00:00 [pmc-release]
AID - 89308870 [pii]
AID - 10.1083/jcb.109.1.309 [doi]
PST - ppublish
SO  - J Cell Biol. 1989 Jul;109(1):309-15. doi: 10.1083/jcb.109.1.309.