PMID- 25261533
OWN - NLM
STAT- MEDLINE
DCOM- 20151105
LR  - 20151119
IS  - 1471-9053 (Electronic)
IS  - 0032-0781 (Linking)
VI  - 56
IP  - 2
DP  - 2015 Feb
TI  - UDP-arabinopyranose mutase 3 is required for pollen wall morphogenesis in rice 
      (Oryza sativa).
PG  - 232-41
LID - 10.1093/pcp/pcu132 [doi]
AB  - l-Arabinose is one of the main constituents of cell wall polysaccharides such as 
      pectic rhamnogalacturonan I (RG-I), glucuronoarabinoxylans and other 
      glycoproteins. It is found predominantly in the furanose form rather than in the 
      thermodynamically more stable pyranose form. UDP-L-arabinofuranose (UDP-Araf), 
      rather than UDP-L-arabinopyranose (UDP-Arap), is a sugar donor for the 
      biosynthesis of arabinofuranosyl (Araf) residues. UDP-arabinopyranose mutases 
      (UAMs) have been shown to interconvert UDP-Araf and UDP-Arap and are involved in 
      the biosynthesis of polysaccharides including Araf. The UAM gene family has three 
      members in Oryza sativa. Co-expression network in silico analysis showed that 
      OsUAM3 expression was independent from OsUAM1 and OsUAM2 co-expression networks. 
      OsUAM1 and OsUAM2 were expressed ubiquitously throughout plant development, but 
      OsUAM3 was expressed primarily in reproductive tissue, particularly at the pollen 
      cell wall formation developmental stage. OsUAM3 co-expression networks include 
      pectin catabolic enzymes. To determine the function of OsUAMs in reproductive 
      tissues, we analyzed RNA interference (RNAi)-knockdown transformants (OsUAM3-KD) 
      specific for OsUAM3. OsUAM3-KD plants grew normally and showed abnormal 
      phenotypes in reproductive tissues, especially in terms of the pollen cell wall 
      and exine. In addition, we examined modifications of cell wall polysaccharides at 
      the cellular level using antibodies against polysaccharides including Araf. 
      Immunolocalization of arabinan using the LM6 antibody showed low levels of 
      arabinan in OsUAM3-KD pollen grains. Our results suggest that the function of 
      OsUAM3 is important for synthesis of arabinan side chains of RG-I and is required 
      for reproductive developmental processes, especially the formation of the cell 
      wall in pollen.
CI  - (c) The Author 2014. Published by Oxford University Press on behalf of Japanese 
      Society of Plant Physiologists. All rights reserved. For permissions, please 
      email: journals.permissions@oup.com.
FAU - Sumiyoshi, Minako
AU  - Sumiyoshi M
AD  - University of Tsukuba, Faculty of Life and Environmental Sciences, Tsukuba, 
      Ibaraki, 305-8572 Japan.
FAU - Inamura, Takuya
AU  - Inamura T
AD  - University of Tsukuba, Faculty of Life and Environmental Sciences, Tsukuba, 
      Ibaraki, 305-8572 Japan.
FAU - Nakamura, Atsuko
AU  - Nakamura A
AD  - University of Tsukuba, Faculty of Life and Environmental Sciences, Tsukuba, 
      Ibaraki, 305-8572 Japan.
FAU - Aohara, Tsutomu
AU  - Aohara T
AD  - University of Tsukuba, Faculty of Life and Environmental Sciences, Tsukuba, 
      Ibaraki, 305-8572 Japan.
FAU - Ishii, Tadashi
AU  - Ishii T
AD  - University of Tsukuba, Faculty of Life and Environmental Sciences, Tsukuba, 
      Ibaraki, 305-8572 Japan.
FAU - Satoh, Shinobu
AU  - Satoh S
AD  - University of Tsukuba, Faculty of Life and Environmental Sciences, Tsukuba, 
      Ibaraki, 305-8572 Japan.
FAU - Iwai, Hiroaki
AU  - Iwai H
AD  - University of Tsukuba, Faculty of Life and Environmental Sciences, Tsukuba, 
      Ibaraki, 305-8572 Japan iwai.hiroaki.gb@u.tsukuba.ac.jp.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140926
PL  - Japan
TA  - Plant Cell Physiol
JT  - Plant & cell physiology
JID - 9430925
RN  - 0 (arabinofuranose)
RN  - B40ROO395Z (Arabinose)
RN  - EC 5.4.- (Intramolecular Transferases)
SB  - IM
MH  - Arabinose/*analogs & derivatives/metabolism
MH  - Cell Wall/*metabolism/ultrastructure
MH  - Gene Expression Regulation, Plant
MH  - Gene Knockdown Techniques
MH  - Intramolecular Transferases/*metabolism
MH  - *Morphogenesis
MH  - Organ Specificity
MH  - Oryza/cytology/*enzymology/genetics/ultrastructure
MH  - Phenotype
MH  - Plants, Genetically Modified
MH  - Pollen/*cytology/*enzymology/ultrastructure
MH  - RNA Interference
MH  - Reproduction
OTO - NOTNLM
OT  - Arabinan
OT  - Intine
OT  - Pectin
OT  - Pollen
OT  - Rice
OT  - UDP-arabinopyranose mutase
EDAT- 2014/09/28 06:00
MHDA- 2015/11/06 06:00
CRDT- 2014/09/28 06:00
PHST- 2014/09/28 06:00 [entrez]
PHST- 2014/09/28 06:00 [pubmed]
PHST- 2015/11/06 06:00 [medline]
AID - pcu132 [pii]
AID - 10.1093/pcp/pcu132 [doi]
PST - ppublish
SO  - Plant Cell Physiol. 2015 Feb;56(2):232-41. doi: 10.1093/pcp/pcu132. Epub 2014 Sep 
      26.