PMID- 25261814
OWN - NLM
STAT- MEDLINE
DCOM- 20150209
LR  - 20171116
IS  - 1879-1026 (Electronic)
IS  - 0048-9697 (Linking)
VI  - 502
DP  - 2015 Jan 1
TI  - A sensitive monoclonal antibody-based enzyme-linked immunosorbent assay for the 
      detection of bifenthrin in a chemical soil barrier.
PG  - 246-51
LID - S0048-9697(14)01342-4 [pii]
LID - 10.1016/j.scitotenv.2014.09.032 [doi]
AB  - A sensitive monoclonal antibody-based enzyme monoclonal antibody-based 
      enzyme-linked immunosorbent assay (ELISA) was developed to detect bifenthrin in a 
      chemical soil barrier for termite control. LBc 
      ((2-methyl[1,1-biphenyl]-3-methoxy) carbonyl propionic acid) was conjugated to 
      bovine serum albumin (BSA) for producing monoclonal antibody. After optimization, 
      the IC50 and limit of detection (LOD, IC10) were 0.05 mg L(-1) and 0.004 mg 
      L(-1), respectively. The sensitivity was improved 40-fold compared to polyclonal 
      antibody-based ELISA reported earlier. No cross-reactivity was measured for the 
      other analogues such as cyhalothrin, cyhalothric acid, cypermethrin, 
      deltamethrin, fenpropathrin, fenvalerate, ethofenprox and tetramethrin except 
      2-methyl-3-biphenylmethyanol. Spiked recoveries were between 83.5% and 104.7% for 
      the detection of bifenthrin in loess, red soil and black soil. All the relative 
      standard deviations (RSDs) were less than or equal to 15.0%. Moreover, the ELISA 
      for authentic samples showed reliability and high correlation with gas 
      chromatography. The developed ELISA is an alternative tool for simple, sensitive 
      and accurate monitoring of bifenthrin in chemical soil barriers.
CI  - Copyright (c) 2014 Elsevier B.V. All rights reserved.
FAU - Hua, Xiude
AU  - Hua X
AD  - College of Plant Protection (State and Local Joint Engineering Research Center of 
      Green Pesticide Invention and Application), Nanjing Agricultural University, 
      Nanjing 210095, China; Key Laboratory of Integrated Management of Crop Diseases 
      and Pests (Nanjing Agricultural University), Ministry of Education, Nanjing, 
      China.
FAU - Liu, Xiaofeng
AU  - Liu X
AD  - College of Plant Protection (State and Local Joint Engineering Research Center of 
      Green Pesticide Invention and Application), Nanjing Agricultural University, 
      Nanjing 210095, China; Key Laboratory of Integrated Management of Crop Diseases 
      and Pests (Nanjing Agricultural University), Ministry of Education, Nanjing, 
      China.
FAU - Yin, Wei
AU  - Yin W
AD  - College of Plant Protection (State and Local Joint Engineering Research Center of 
      Green Pesticide Invention and Application), Nanjing Agricultural University, 
      Nanjing 210095, China; Key Laboratory of Integrated Management of Crop Diseases 
      and Pests (Nanjing Agricultural University), Ministry of Education, Nanjing, 
      China.
FAU - Xia, Yazhong
AU  - Xia Y
AD  - Wuxi Termite Control Center, Wuxi 214071, China.
FAU - Zhou, Qiujun
AU  - Zhou Q
AD  - Wuxi Termite Control Center, Wuxi 214071, China.
FAU - Lu, Yiwen
AU  - Lu Y
AD  - Wuxi Termite Control Center, Wuxi 214071, China.
FAU - Li, Wei
AU  - Li W
AD  - Wuxi Termite Control Center, Wuxi 214071, China.
FAU - Shi, Haiyan
AU  - Shi H
AD  - College of Plant Protection (State and Local Joint Engineering Research Center of 
      Green Pesticide Invention and Application), Nanjing Agricultural University, 
      Nanjing 210095, China; Key Laboratory of Integrated Management of Crop Diseases 
      and Pests (Nanjing Agricultural University), Ministry of Education, Nanjing, 
      China.
FAU - Liu, Fengquan
AU  - Liu F
AD  - College of Plant Protection (State and Local Joint Engineering Research Center of 
      Green Pesticide Invention and Application), Nanjing Agricultural University, 
      Nanjing 210095, China; Institute of Plant Protection, Jiangsu Academy of 
      Agricultural Science, Nanjing 210014, China.
FAU - Wang, Minghua
AU  - Wang M
AD  - College of Plant Protection (State and Local Joint Engineering Research Center of 
      Green Pesticide Invention and Application), Nanjing Agricultural University, 
      Nanjing 210095, China; Key Laboratory of Integrated Management of Crop Diseases 
      and Pests (Nanjing Agricultural University), Ministry of Education, Nanjing, 
      China. Electronic address: wangmha@njau.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140926
PL  - Netherlands
TA  - Sci Total Environ
JT  - The Science of the total environment
JID - 0330500
RN  - 0 (Insecticides)
RN  - 0 (Pyrethrins)
RN  - 0 (Soil)
RN  - 0 (Soil Pollutants)
RN  - 6B66JED0KN (bifenthrin)
SB  - IM
MH  - Environmental Monitoring/*methods
MH  - Enzyme-Linked Immunosorbent Assay/*methods
MH  - Insecticides/*analysis
MH  - Limit of Detection
MH  - Pyrethrins/*analysis
MH  - Reproducibility of Results
MH  - Soil/*chemistry
MH  - Soil Pollutants/*analysis
OTO - NOTNLM
OT  - Bifenthrin
OT  - Chemical soil barrier
OT  - Enzyme-linked immunosorbent assay
OT  - Monoclonal antibody
EDAT- 2014/09/28 06:00
MHDA- 2015/02/11 06:00
CRDT- 2014/09/28 06:00
PHST- 2014/07/31 00:00 [received]
PHST- 2014/09/11 00:00 [revised]
PHST- 2014/09/11 00:00 [accepted]
PHST- 2014/09/28 06:00 [entrez]
PHST- 2014/09/28 06:00 [pubmed]
PHST- 2015/02/11 06:00 [medline]
AID - S0048-9697(14)01342-4 [pii]
AID - 10.1016/j.scitotenv.2014.09.032 [doi]
PST - ppublish
SO  - Sci Total Environ. 2015 Jan 1;502:246-51. doi: 10.1016/j.scitotenv.2014.09.032. 
      Epub 2014 Sep 26.