PMID- 25262156
OWN - NLM
STAT- MEDLINE
DCOM- 20150202
LR  - 20141202
IS  - 1872-8421 (Electronic)
IS  - 0165-5728 (Linking)
VI  - 276
IP  - 1-2
DP  - 2014 Nov 15
TI  - Expression of extracellular domains of muscle specific kinase (MuSK) and use as 
      immunoadsorbents for the development of an antigen-specific therapy.
PG  - 150-8
LID - S0165-5728(14)00890-X [pii]
LID - 10.1016/j.jneuroim.2014.09.013 [doi]
AB  - Antibodies against MuSK seem to be the pathogenic factor in approximately 5-8% of 
      myasthenia gravis (MG) patients. We aim to develop an antigen-specific therapy in 
      which only MuSK antibodies will be removed from patients' plasma using MuSK 
      extracellular domain (MuSK-ECD) as immunoadsorbent. We showed that two different 
      immunoadsorbents, very efficiently and selectively depleted the MuSK antibodies 
      from all tested sera, were stable during the procedure and were reusable. 
      Furthermore, animal experiments showed that the treatment has no toxic effects to 
      the animals. We conclude that the MuSK-ECD-mediated immunoadsorption can be used 
      as an efficient antigen-specific therapy for MuSK-MG.
CI  - Copyright (c) 2014 Elsevier B.V. All rights reserved.
FAU - Skriapa, Lamprini
AU  - Skriapa L
AD  - Department of Neurobiology, Hellenic Pasteur Institute, Athens, Greece; 
      Department of Pharmacy, University of Patras, Rio, Greece.
FAU - Zisimopoulou, Paraskevi
AU  - Zisimopoulou P
AD  - Department of Neurobiology, Hellenic Pasteur Institute, Athens, Greece. 
      Electronic address: zisimopoulou@pasteur.gr.
FAU - Trakas, Nikolaos
AU  - Trakas N
AD  - Department of Neurobiology, Hellenic Pasteur Institute, Athens, Greece.
FAU - Grapsa, Eirini
AU  - Grapsa E
AD  - Department of Nephrology, Aretaieion Hospital, Athens, Greece.
FAU - Tzartos, Socrates J
AU  - Tzartos SJ
AD  - Department of Neurobiology, Hellenic Pasteur Institute, Athens, Greece; 
      Department of Pharmacy, University of Patras, Rio, Greece. Electronic address: 
      tzartos@pasteur.gr.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140919
PL  - Netherlands
TA  - J Neuroimmunol
JT  - Journal of neuroimmunology
JID - 8109498
RN  - 0 (Autoantibodies)
RN  - 0 (Immunosorbents)
RN  - 0 (Receptors, Cholinergic)
RN  - EC 2.7.10.1 (MUSK protein, human)
RN  - EC 2.7.10.1 (Receptor Protein-Tyrosine Kinases)
SB  - IM
MH  - Animals
MH  - Autoantibodies/blood/*therapeutic use
MH  - Diaphragm/metabolism/pathology
MH  - Female
MH  - Humans
MH  - Immunization, Passive/*methods
MH  - Immunosorbent Techniques
MH  - Immunosorbents/*therapeutic use
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Myasthenia Gravis/blood/*immunology
MH  - Radioimmunoassay
MH  - Receptor Protein-Tyrosine Kinases/blood/genetics/*immunology
MH  - Receptors, Cholinergic/blood/genetics/*immunology
OTO - NOTNLM
OT  - Autoimmune diseases
OT  - Extracellular domains
OT  - Immunoadsorption
OT  - Immunotherapy
OT  - MuSK antibodies
OT  - Myasthenia gravis
EDAT- 2014/09/30 06:00
MHDA- 2015/02/03 06:00
CRDT- 2014/09/29 06:00
PHST- 2014/06/18 00:00 [received]
PHST- 2014/09/12 00:00 [accepted]
PHST- 2014/09/29 06:00 [entrez]
PHST- 2014/09/30 06:00 [pubmed]
PHST- 2015/02/03 06:00 [medline]
AID - S0165-5728(14)00890-X [pii]
AID - 10.1016/j.jneuroim.2014.09.013 [doi]
PST - ppublish
SO  - J Neuroimmunol. 2014 Nov 15;276(1-2):150-8. doi: 10.1016/j.jneuroim.2014.09.013. 
      Epub 2014 Sep 19.