PMID- 25264527
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20140929
LR  - 20211021
IS  - 2287-8572 (Print)
IS  - 2287-8580 (Electronic)
IS  - 2287-8572 (Linking)
VI  - 57
IP  - 5
DP  - 2014 Sep
TI  - Dose-dependent embryotrophic effect of recombinant granulocyte-macrophage 
      colony-stimulating factor and brain-derived neurotrophic factor in culture medium 
      for mouse preimplantation embryo.
PG  - 373-8
LID - 10.5468/ogs.2014.57.5.373 [doi]
AB  - OBJECTIVE: To evaluate the dose effect of recombinant mouse 
      granulocyte-macrophage colony-stimulating factor (rmGM-CSF) or brain-derived 
      neurotrophic factor (BDNF) in culture medium on the development of in vitro 
      fertilized mouse embryos. METHODS: Mature oocytes were retrieved from 
      superovulated female BDF1 mice and inseminated by sperm from male BDF1 mice. On 
      day 1, two-cell stage embryos were divided and cultured until day 5 in the embryo 
      maintenance medium supplemented with 0, 1, 2, 5, or 10 ng/mL of rmGM-CSF or 
      supplemented with 0, 5, 10, or 20 ng/mL of BDNF. Blastocyst formation rate and 
      their cell numbers were assessed. RESULTS: The blastocyst formation rate and the 
      total cell count in blastocyst was similar in all the rmGM-CSF treatment groups 
      when compared with the control. However, the blastocyst formation rate and the 
      total cell count was significantly higher in the group supplemented with 10 ng/mL 
      of BDNF compared with the control (63.9%, 45.8+/-11.5 vs. 52.3%, 38.0+/-6.8; P<0.05, 
      respectively). CONCLUSION: Supplementation of 10 ng/mL of BDNF enhanced the 
      developmental potential of mouse preimplantation embryos, but supplementation of 
      rmGM-CSF did not.
FAU - Kim, Jee Hyun
AU  - Kim JH
AD  - Department of Obstetrics and Gynecology, Seoul National University Bundang 
      Hospital, Seongnam, Korea. ; Department of Obstetrics and Gynecology, Seoul 
      National University College of Medicine, Seoul, Korea.
FAU - Lee, Hyun Ju
AU  - Lee HJ
AD  - Department of Obstetrics and Gynecology, Seoul National University Hospital, 
      Seoul, Korea.
FAU - Yu, Eun Jeong
AU  - Yu EJ
AD  - Department of Obstetrics and Gynecology, Seoul National University Hospital, 
      Seoul, Korea.
FAU - Jee, Byung Chul
AU  - Jee BC
AD  - Department of Obstetrics and Gynecology, Seoul National University Bundang 
      Hospital, Seongnam, Korea. ; Department of Obstetrics and Gynecology, Seoul 
      National University College of Medicine, Seoul, Korea.
FAU - Suh, Chang Suk
AU  - Suh CS
AD  - Department of Obstetrics and Gynecology, Seoul National University Bundang 
      Hospital, Seongnam, Korea. ; Department of Obstetrics and Gynecology, Seoul 
      National University College of Medicine, Seoul, Korea.
FAU - Kim, Seok Hyun
AU  - Kim SH
AD  - Department of Obstetrics and Gynecology, Seoul National University College of 
      Medicine, Seoul, Korea. ; Department of Obstetrics and Gynecology, Seoul National 
      University Hospital, Seoul, Korea.
LA  - eng
PT  - Journal Article
DEP - 20140917
PL  - Korea (South)
TA  - Obstet Gynecol Sci
JT  - Obstetrics & gynecology science
JID - 101602614
PMC - PMC4175597
OTO - NOTNLM
OT  - Brain-derived neurotrophic factor
OT  - Culture medium
OT  - Embryotrophic effects
OT  - Granulocyte-macrophage colony-stimulating factor
COIS- No potential conflict of interest relevant to this article was reported.
EDAT- 2014/09/30 06:00
MHDA- 2014/09/30 06:01
PMCR- 2014/09/01
CRDT- 2014/09/30 06:00
PHST- 2013/11/27 00:00 [received]
PHST- 2014/04/23 00:00 [revised]
PHST- 2014/05/19 00:00 [accepted]
PHST- 2014/09/30 06:00 [entrez]
PHST- 2014/09/30 06:00 [pubmed]
PHST- 2014/09/30 06:01 [medline]
PHST- 2014/09/01 00:00 [pmc-release]
AID - 10.5468/ogs.2014.57.5.373 [doi]
PST - ppublish
SO  - Obstet Gynecol Sci. 2014 Sep;57(5):373-8. doi: 10.5468/ogs.2014.57.5.373. Epub 
      2014 Sep 17.