PMID- 25264576
OWN - NLM
STAT- MEDLINE
DCOM- 20151214
LR  - 20191210
IS  - 1872-7549 (Electronic)
IS  - 0166-4328 (Linking)
VI  - 278
DP  - 2015 Feb 1
TI  - Contextual fear conditioning modulates hippocampal AMPA-, GluN1- and serotonin 
      receptor 5-HT1A-containing receptor complexes.
PG  - 44-54
LID - S0166-4328(14)00633-0 [pii]
LID - 10.1016/j.bbr.2014.09.035 [doi]
AB  - Although the roles of AMPAR (alpha-amino-3-hydroxyl-5-methyl-4-isoxazole propionate 
      receptor), NMDAR (N-methyl-D-aspartate receptor) and 5HT1AR (5-hydroxytryptamine 
      sub type 1A) in contextual fear conditioning (cFC) have been studied, information 
      about receptor-containing complexes (RC) is not available. Moreover, there are no 
      data on membrane or endosomal NMDA-, 5HT1A- or AMPA-RC levels, which would likely 
      be indicative of the trafficking of these receptors. cFC was carried out in 
      C57BL/6j mice and animals were sacrificed in the individual phases and hippocampi 
      were taken for the determination of receptor complex and subunit levels using BN- 
      and SDS-PAGE with subsequent Western blotting. GluA1-4, GluN1 (NMDAR subunit 
      NR1)- and 5HT1A-RC were differentially regulated during the individual phases and 
      differentially regulated in the membrane and endosomal fractions. GluA1-RC levels 
      in the membrane were increased in acquisition, consolidation and retrieval 
      phases; GluA2-RC and GluA3-RC membrane levels were reduced and modulated in early 
      endosomes during these phases. GluA4-RC and GluN1-RC levels as well as their 
      subunits showed the same pattern in the membrane during consolidation while 
      5HT1A-RC membrane and endosome levels were mainly increased during consolidation 
      and retrieval. Taken together, the results suggest that levels of 5-HT1A-RC, 
      NMDA-RC and AMPA-RC and subunits in membrane and endosomal preparations are 
      paralleling individual phases of cFC. The findings from the current study suggest 
      phase-specific receptor complex and subunit formation and propose that receptor 
      complexes should be examined in parallel with receptor subunits to aid the 
      interpretation of previous work and to design future work on neurotransmitter 
      receptors in memory paradigms.
CI  - Copyright (c) 2014 Elsevier B.V. All rights reserved.
FAU - Sase, Sunetra
AU  - Sase S
AD  - Department of Pediatrics, Medical University of Vienna, 1090 Vienna, Austria.
FAU - Stork, Oliver
AU  - Stork O
AD  - Institute of Biology, Otto von Guericke University, Magdeburg, 39120 Magdeburg, 
      Germany.
FAU - Lubec, Gert
AU  - Lubec G
AD  - Department of Pediatrics, Medical University of Vienna, 1090 Vienna, Austria. 
      Electronic address: gert.lubec@meduniwien.ac.at.
FAU - Li, Lin
AU  - Li L
AD  - Department of Pediatrics, Medical University of Vienna, 1090 Vienna, Austria. 
      Electronic address: linli@gmail.com.
LA  - eng
PT  - Journal Article
DEP - 20141001
PL  - Netherlands
TA  - Behav Brain Res
JT  - Behavioural brain research
JID - 8004872
RN  - 0 (Gprin1 protein, mouse)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Receptors, AMPA)
RN  - 0 (Receptors, N-Methyl-D-Aspartate)
RN  - 112692-38-3 (Receptor, Serotonin, 5-HT1A)
SB  - IM
MH  - Animals
MH  - Cell Membrane/metabolism
MH  - *Conditioning, Psychological
MH  - Electroshock/adverse effects
MH  - Endosomes/metabolism
MH  - *Fear
MH  - Freezing Reaction, Cataleptic
MH  - Gene Expression Regulation
MH  - Hippocampus/*metabolism/ultrastructure
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Nerve Tissue Proteins/*metabolism
MH  - Receptor, Serotonin, 5-HT1A/*metabolism
MH  - Receptors, AMPA/*metabolism
MH  - Receptors, N-Methyl-D-Aspartate/*metabolism
MH  - Statistics, Nonparametric
OTO - NOTNLM
OT  - 5-HT1(A) receptor
OT  - AMPA receptor
OT  - Contextual fear conditioning
OT  - NMDA receptor
OT  - Receptor complex
EDAT- 2014/09/30 06:00
MHDA- 2015/12/15 06:00
CRDT- 2014/09/30 06:00
PHST- 2014/08/13 00:00 [received]
PHST- 2014/09/16 00:00 [revised]
PHST- 2014/09/20 00:00 [accepted]
PHST- 2014/09/30 06:00 [entrez]
PHST- 2014/09/30 06:00 [pubmed]
PHST- 2015/12/15 06:00 [medline]
AID - S0166-4328(14)00633-0 [pii]
AID - 10.1016/j.bbr.2014.09.035 [doi]
PST - ppublish
SO  - Behav Brain Res. 2015 Feb 1;278:44-54. doi: 10.1016/j.bbr.2014.09.035. Epub 2014 
      Oct 1.