PMID- 25266001
OWN - NLM
STAT- MEDLINE
DCOM- 20150610
LR  - 20140930
IS  - 1735-367X (Electronic)
IS  - 1735-1383 (Linking)
VI  - 11
IP  - 3
DP  - 2014 Sep
TI  - Maturation state and function of monocyte derived dendritic cells in liver 
      transplant recipients.
PG  - 153-65
AB  - BACKGROUND: Dendritic cells (DCs) are potent antigen presenting cells for 
      triggering of the immune reaction post transplantation. These cells are centrally 
      involved in the initiation of T cell-dependent immune responses. OBJECTIVE: To 
      compare the level of DC maturation and function in liver transplant recipients 
      with healthy controls. METHODS: In this study, twelve peripheral blood samples 
      were selected from six liver transplant patients and six healthy controls. After 
      the generation of DCs from monocytes, expression levels and mean fluorescent 
      intensity (MFI) of several DC maturation markers were evaluated using flow 
      cytometry. Secretion of IL-6, IL-12 and IL-23 proinflammatory cytokines was 
      determined using ELISA. Gene expressions of TLR-2, TLR-4 and IL-23 were analyzed 
      using real-time PCR. RESULTS: DC expression markers including CD83 (p=0.007) and 
      CD86 (p=0.02), as well as secretion of IL-6 (p=0.02) and IL-12 (p=0.007) by DCs 
      were significantly increased in liver transplant patients compared with healthy 
      controls. The MFI of CD86 (p=0.009) and HLA-DR (p= 0.005) expression on DCs was 
      also higher in patients. The expression of TLR-2 transcripts in DCs of patients 
      was higher than that of the controls (p=0.03). CONCLUSION: Based on these 
      findings, increased frequency of DCs expressing CD83 and CD86, higher expression 
      of CD86, HLA-DR, and TLR-2 as well as elevated secretion of proinflammatory 
      cytokines in DCs of liver transplant recipient's point to the more mature 
      phenotype and active function of DCs in patients compared with controls.
FAU - Shariat, Afsoon
AU  - Shariat A
AD  - Department of Biology, College of Basic Sciences, Tehran Science and Research 
      Branch, Islamic Azad University, Tehran, Iran,e-mail:afsoonsh1980@yahoo.com.
FAU - Karimi, Mohammad Hossein
AU  - Karimi MH
FAU - Mokhtariazad, Talat
AU  - Mokhtariazad T
FAU - Moazzeni, Seyed Mohammad
AU  - Moazzeni SM
FAU - Geramizadeh, Bita
AU  - Geramizadeh B
FAU - MalekHosseini, Seyed Ali
AU  - MalekHosseini SA
FAU - Yaghobi, Ramin
AU  - Yaghobi R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Iran
TA  - Iran J Immunol
JT  - Iranian journal of immunology : IJI
JID - 101282932
RN  - 0 (Antigens, Surface)
RN  - 0 (Cytokines)
RN  - 0 (Toll-Like Receptor 2)
RN  - 0 (Toll-Like Receptor 4)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Antigens, Surface/metabolism
MH  - Case-Control Studies
MH  - *Cell Differentiation
MH  - Cytokines/biosynthesis/genetics
MH  - Dendritic Cells/*cytology/*physiology
MH  - Female
MH  - Gene Expression
MH  - Humans
MH  - Immunophenotyping
MH  - *Liver Transplantation
MH  - Male
MH  - Middle Aged
MH  - Monocytes/*cytology/metabolism
MH  - Toll-Like Receptor 2/genetics/metabolism
MH  - Toll-Like Receptor 4/genetics/metabolism
MH  - *Transplant Recipients
MH  - Young Adult
EDAT- 2014/10/01 06:00
MHDA- 2015/06/11 06:00
CRDT- 2014/10/01 06:00
PHST- 2014/10/01 06:00 [entrez]
PHST- 2014/10/01 06:00 [pubmed]
PHST- 2015/06/11 06:00 [medline]
AID - 02 [pii]
PST - ppublish
SO  - Iran J Immunol. 2014 Sep;11(3):153-65.