PMID- 25267883
OWN - NLM
STAT- MEDLINE
DCOM- 20151117
LR  - 20150220
IS  - 1460-2377 (Electronic)
IS  - 0953-8178 (Linking)
VI  - 27
IP  - 3
DP  - 2015 Mar
TI  - IL-10 enhances the phenotype of M2 macrophages induced by IL-4 and confers the 
      ability to increase eosinophil migration.
PG  - 131-41
LID - 10.1093/intimm/dxu090 [doi]
AB  - M2 macrophages have been subdivided into subtypes such as IL-4-induced M2a and 
      IL-10-induced M2c in vitro. Although it was reported that IL-10 stimulation leads 
      to an increase in IL-4Ralpha, the effect of IL-4 and IL-10 in combination with 
      macrophage subtype differentiation remains unclear. Thus, we sought to clarify 
      whether IL-10 enhanced the M2 phenotype induced by IL-4. In this study, we showed 
      that IL-10 enhanced IL-4Ralpha expression in M-CSF-induced bone marrow-derived 
      macrophages (BMDMs). Global gene expression analysis of M2 macrophages induced by 
      IL-4, IL-10 or IL-4 + IL-10 showed that IL-10 enhanced gene expression of M2a 
      markers induced by IL-4 in M-CSF-induced BMDMs. Moreover, IL-4 and IL-10 
      synergistically induced CCL24 (Eotaxin-2) production. Enhanced CCL24 expression 
      was also observed in GM-CSF-induced BMDMs and zymosan-elicited, 
      thioglycolate-elicited and naive peritoneal macrophages. CCL24 is a CCR3 agonist 
      and an eosinophil chemoattractant. In vitro, IL-4 + IL-10-stimulated macrophages 
      produced a large amount of CCL24 and increased eosinophil migration, which was 
      inhibited by anti-CCL24 antibody. We also showed that IL-4 + IL-10-stimulated 
      (but not IL-4 or IL-10 alone) macrophages transferred into the peritoneum of 
      C57BL/6J mice increased eosinophil infiltration into the peritoneal cavity. These 
      results demonstrate that IL-4 + IL-10-simulated macrophages have enhanced M2a 
      macrophage-related gene expression, CCL24 production and eosinophil 
      infiltration-inducing activity, thereby suggesting their contribution to 
      eosinophil-related diseases.
CI  - (c) The Japanese Society for Immunology. 2014. All rights reserved. For 
      permissions, please e-mail: journals.permissions@oup.com.
FAU - Makita, Naoyuki
AU  - Makita N
AD  - Faculty of Exploratory Pharmacology, Asubio Pharma Co., Ltd., 6-4-3, 
      Minatojima-Minamimachi, Chuo-ku, Kobe 650-0047, Japan.
FAU - Hizukuri, Yoshiyuki
AU  - Hizukuri Y
AD  - Faculty of Exploratory Technology, Asubio Pharma Co., Ltd., 6-4-3, 
      Minatojima-Minamimachi, Chuo-ku, Kobe 650-0047, Japan.
FAU - Yamashiro, Kyoko
AU  - Yamashiro K
AD  - Faculty of Exploratory Technology, Asubio Pharma Co., Ltd., 6-4-3, 
      Minatojima-Minamimachi, Chuo-ku, Kobe 650-0047, Japan.
FAU - Murakawa, Masao
AU  - Murakawa M
AD  - Faculty of Exploratory Pharmacology, Asubio Pharma Co., Ltd., 6-4-3, 
      Minatojima-Minamimachi, Chuo-ku, Kobe 650-0047, Japan.
FAU - Hayashi, Yasuhiro
AU  - Hayashi Y
AD  - Faculty of Exploratory Technology, Asubio Pharma Co., Ltd., 6-4-3, 
      Minatojima-Minamimachi, Chuo-ku, Kobe 650-0047, Japan 
      hayashi.yasuhiro.ej@asubio.co.jp.
LA  - eng
PT  - Journal Article
DEP - 20140929
PL  - England
TA  - Int Immunol
JT  - International immunology
JID - 8916182
RN  - 0 (Antibodies, Blocking)
RN  - 0 (Chemokine CCL4)
RN  - 0 (Il4ra protein, mouse)
RN  - 0 (Receptors, Cell Surface)
RN  - 130068-27-8 (Interleukin-10)
RN  - 207137-56-2 (Interleukin-4)
RN  - 81627-83-0 (Macrophage Colony-Stimulating Factor)
SB  - IM
MH  - Animals
MH  - Antibodies, Blocking/pharmacology
MH  - Cell Differentiation
MH  - Cell Movement/drug effects
MH  - Cells, Cultured
MH  - Chemokine CCL4/genetics/*metabolism
MH  - Eosinophils/*immunology
MH  - Interleukin-10/immunology/*metabolism
MH  - Interleukin-4/immunology/*metabolism
MH  - Macrophage Colony-Stimulating Factor/immunology
MH  - Macrophages/*immunology/transplantation
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred C57BL
MH  - Microarray Analysis
MH  - Phenotype
MH  - Receptors, Cell Surface/genetics/metabolism
MH  - Up-Regulation
OTO - NOTNLM
OT  - CCL24
OT  - IL-10
OT  - IL-4
OT  - M2a macrophage
OT  - eosinophil infiltration
EDAT- 2014/10/01 06:00
MHDA- 2015/11/18 06:00
CRDT- 2014/10/01 06:00
PHST- 2014/10/01 06:00 [entrez]
PHST- 2014/10/01 06:00 [pubmed]
PHST- 2015/11/18 06:00 [medline]
AID - dxu090 [pii]
AID - 10.1093/intimm/dxu090 [doi]
PST - ppublish
SO  - Int Immunol. 2015 Mar;27(3):131-41. doi: 10.1093/intimm/dxu090. Epub 2014 Sep 29.