PMID- 25269089
OWN - NLM
STAT- MEDLINE
DCOM- 20150720
LR  - 20141202
IS  - 1873-3492 (Electronic)
IS  - 0009-8981 (Linking)
VI  - 438
DP  - 2015 Jan 1
TI  - An improved flow cytometric immunobead array to detect autoantibodies in plasma 
      from patients with immune thrombocytopenic purpura.
PG  - 396-400
LID - S0009-8981(14)00414-8 [pii]
LID - 10.1016/j.cca.2014.09.018 [doi]
AB  - BACKGROUND: Autoantibodies against platelet glycoproteins (GPs) play an important 
      role in immune thrombocytopenic purpura (ITP). This study was to develop an 
      improved flow cytometric immunobead array (FCIA) assay to detect platelet 
      autoantibodies in ITP patient plasma. METHODS: Plasma samples were isolated from 
      71 ITP patients and 136 non-ITP controls and incubated with platelets from normal 
      individuals. After washing, platelets were lysed and the platelet lysates were 
      incubated with polystyrene microbeads coupled with monoclonal antibodies against 
      human GPs IX (SZ1), Ib (SZ2), IIIa (SZ21), IIb (SZ22), and P-selectin (SZ51). 
      Platelet GP-autoantibody complexes were detected by flow cytometry using a 
      FITC-labeled secondary antibody. RESULT: Autoantibodies against platelet GPIb, 
      GPIIb, GPIIIa, GPIX and P-selectin were detected in plasma from ITP patients, as 
      indicated by high mean fluorescent intensity values when microbeads with 
      antibodies SZ1, SZ2, SZ21, SZ22, and SZ51 were used. In ROC analysis, values of 
      the area under the curve were 0.74, 0.83, 0.80, 0.79 and 0.87, respectively. 
      Compared with the previously reported assays, this new FCIA eliminated the need 
      of isolating platelets from ITP patients without compromising assay sensitivity 
      and accuracy in predicting ITP. CONCLUSION: This simplified FICA assay may be 
      more suitable for ITP diagnosis in clinical laboratory settings.
CI  - Copyright (c) 2014 Elsevier B.V. All rights reserved.
FAU - Zhao, Yunxiao
AU  - Zhao Y
AD  - MOH Key Lab of Thrombosis and Hemostasis, Jiangsu Institute of Hematology, the 
      First Affiliated Hospital, the Cyrus Tang Hematology Center, and the 
      Collaborative Innovation Center of Hematology, Soochow University, Suzhou, China.
FAU - Zhu, Mingqing
AU  - Zhu M
AD  - MOH Key Lab of Thrombosis and Hemostasis, Jiangsu Institute of Hematology, the 
      First Affiliated Hospital, the Cyrus Tang Hematology Center, and the 
      Collaborative Innovation Center of Hematology, Soochow University, Suzhou, China.
FAU - Jiang, Miao
AU  - Jiang M
AD  - MOH Key Lab of Thrombosis and Hemostasis, Jiangsu Institute of Hematology, the 
      First Affiliated Hospital, the Cyrus Tang Hematology Center, and the 
      Collaborative Innovation Center of Hematology, Soochow University, Suzhou, China.
FAU - Zuo, Bin
AU  - Zuo B
AD  - MOH Key Lab of Thrombosis and Hemostasis, Jiangsu Institute of Hematology, the 
      First Affiliated Hospital, the Cyrus Tang Hematology Center, and the 
      Collaborative Innovation Center of Hematology, Soochow University, Suzhou, China.
FAU - Wu, Qingyu
AU  - Wu Q
AD  - MOH Key Lab of Thrombosis and Hemostasis, Jiangsu Institute of Hematology, the 
      First Affiliated Hospital, the Cyrus Tang Hematology Center, and the 
      Collaborative Innovation Center of Hematology, Soochow University, Suzhou, China.
FAU - Ruan, Changgeng
AU  - Ruan C
AD  - MOH Key Lab of Thrombosis and Hemostasis, Jiangsu Institute of Hematology, the 
      First Affiliated Hospital, the Cyrus Tang Hematology Center, and the 
      Collaborative Innovation Center of Hematology, Soochow University, Suzhou, China.
FAU - He, Yang
AU  - He Y
AD  - MOH Key Lab of Thrombosis and Hemostasis, Jiangsu Institute of Hematology, the 
      First Affiliated Hospital, the Cyrus Tang Hematology Center, and the 
      Collaborative Innovation Center of Hematology, Soochow University, Suzhou, China. 
      Electronic address: heyang1963@163.com.
LA  - eng
PT  - Journal Article
DEP - 20140928
PL  - Netherlands
TA  - Clin Chim Acta
JT  - Clinica chimica acta; international journal of clinical chemistry
JID - 1302422
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Autoantibodies)
RN  - 0 (Platelet Membrane Glycoproteins)
RN  - 0 (Polystyrenes)
RN  - I223NX31W9 (Fluorescein-5-isothiocyanate)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Antibodies, Monoclonal
MH  - Area Under Curve
MH  - Autoantibodies/*blood
MH  - Blood Platelets/chemistry/immunology
MH  - Case-Control Studies
MH  - Child
MH  - Child, Preschool
MH  - Female
MH  - Flow Cytometry/instrumentation/*standards
MH  - Fluorescein-5-isothiocyanate
MH  - Humans
MH  - Immunoassay/instrumentation/*standards
MH  - Male
MH  - Microspheres
MH  - Middle Aged
MH  - Platelet Membrane Glycoproteins/*analysis/immunology
MH  - Polystyrenes
MH  - Protein Array Analysis/instrumentation/*standards
MH  - Purpura, Thrombocytopenic, Idiopathic/blood/*diagnosis/immunology
MH  - ROC Curve
MH  - Staining and Labeling/methods
OTO - NOTNLM
OT  - Flow cytometry
OT  - Immune thrombocytopenic purpure
OT  - Immunebead array
OT  - Platelet glycoprotein
OT  - Thrombocytopenia
EDAT- 2014/10/01 06:00
MHDA- 2015/07/21 06:00
CRDT- 2014/10/01 06:00
PHST- 2014/03/02 00:00 [received]
PHST- 2014/09/12 00:00 [revised]
PHST- 2014/09/15 00:00 [accepted]
PHST- 2014/10/01 06:00 [entrez]
PHST- 2014/10/01 06:00 [pubmed]
PHST- 2015/07/21 06:00 [medline]
AID - S0009-8981(14)00414-8 [pii]
AID - 10.1016/j.cca.2014.09.018 [doi]
PST - ppublish
SO  - Clin Chim Acta. 2015 Jan 1;438:396-400. doi: 10.1016/j.cca.2014.09.018. Epub 2014 
      Sep 28.