PMID- 25269254
OWN - NLM
STAT- MEDLINE
DCOM- 20150901
LR  - 20190923
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 32
IP  - 6
DP  - 2014 Jun
TI  - [Determination of gossypol in edible vegetable oil with high performance liquid 
      chromatography-tandem mass spectrometry].
PG  - 582-5
AB  - A liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed 
      for the determination of gossypol in edible vegetable oil. The sample was 
      extracted with ethyl alcohol by vortex-excited oscillation. The extract was 
      cleaned up by 0.22 microm filter membrane and centrifuged for 5 min at 4 000 
      r/min after standing in a fridge at 4 degrees C for 30 min. The compound was 
      separated on a C18 column (100 mm x 2.1 mm, 3.5 microm) with acetonitrile and 1% 
      (v/v) formic acid aqueous solution as mobile phase. The detection of gossypol was 
      carried out by LC-MS/MS with positive electrospray ionization under multiple 
      reaction monitoring (MRM) mode using external standard method. The limits of 
      quantification (S/N > 10) of gossypol in edible vegetable oil was 1 mg/kg. The 
      recoveries were from 87.4% to 100% at the spiked levels of 1, 2, 200 mg/kg of 
      gossypol in edible vegetable oil with the relative standard deviations (RSDs) 
      between 3.9% and 12.2%. The method, with high sensitivity, good precision and 
      high recovery, was suitable for the confirmation and quantification of gossypol 
      residue in edible vegetable oil.
FAU - Zhang, Wenhua
AU  - Zhang W
FAU - Huang, Chaoqun
AU  - Huang C
FAU - Xie, Wen
AU  - Xie W
FAU - Shen, Li
AU  - Shen L
LA  - chi
PT  - Journal Article
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 0 (Plant Oils)
RN  - KAV15B369O (Gossypol)
SB  - IM
MH  - Chromatography, High Pressure Liquid
MH  - Gossypol/*analysis
MH  - Plant Oils/*analysis
MH  - Tandem Mass Spectrometry
MH  - *Vegetables
EDAT- 2014/10/02 06:00
MHDA- 2015/09/02 06:00
CRDT- 2014/10/02 06:00
PHST- 2014/10/02 06:00 [entrez]
PHST- 2014/10/02 06:00 [pubmed]
PHST- 2015/09/02 06:00 [medline]
AID - 10.3724/sp.j.1123.2014.02029 [doi]
PST - ppublish
SO  - Se Pu. 2014 Jun;32(6):582-5. doi: 10.3724/sp.j.1123.2014.02029.