PMID- 25269256
OWN - NLM
STAT- MEDLINE
DCOM- 20150901
LR  - 20190923
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 32
IP  - 6
DP  - 2014 Jun
TI  - [Qualitative and quantitative analysis of amygdalin and its metabolite prunasin 
      in plasma by ultra-high performance liquid chromatography-tandem quadrupole time 
      of flight mass spectrometry and ultra-high performance liquid 
      chromatography-tandem triple quadrupole mass spectrometry].
PG  - 591-9
AB  - A method was developed for the determination of amygdalin and its metabolite 
      prunasin in rat plasma after intragastric administration of Maxing shigan 
      decoction. The analytes were identified by ultra-high performance liquid 
      chromatography-tandem quadrupole time of flight mass spectrometry and 
      quantitatively determined by ultra-high performance liquid chromatography-tandem 
      triple quadrupole mass spectrometry. After purified by liquid-liquid extraction, 
      the qualitative analysis of amygdalin and prunasin in the plasma sample was 
      performed on a Shim-pack XR-ODS III HPLC column (75 mm x 2.0 mm, 1.6 microm), 
      using acetonitrile-0.1% (v/v) formic acid aqueous solution. The detection was 
      performed on a Triple TOF 5600 quadrupole time of flight mass spectrometer. The 
      quantitative analysis of amygdalin and prunasin in the plasma sample was 
      performed by separation on an Agilent C18 HPLC column (50 mm x 2.1 mm, 1.7 
      microm), using acetonitrile-0.1% (v/v) formic acid aqueous solution. The 
      detection was performed on an AB Q-TRAP 4500 triple quadrupole mass spectrometer 
      utilizing electrospray ionization (ESI) interface operated in negative ion mode 
      and multiple-reaction monitoring (MRM) mode. The qualitative analysis results 
      showed that amygdalin and its metabolite prunasin were detected in the plasma 
      sample. The quantitative analysis results showed that the linear range of 
      amygdalin was 1.05-4 200 ng/mL with the correlation coefficient of 0.999 0 and 
      the linear range of prunasin was 1.25-2 490 ng/mL with the correlation 
      coefficient of 0.997 0. The method had a good precision with the relative 
      standard deviations (RSDs) lower than 9.20% and the overall recoveries varied 
      from 82.33% to 95.25%. The limits of detection (LODs) of amygdalin and prunasin 
      were 0.50 ng/mL. With good reproducibility, the method is simple, fast and 
      effective for the qualitative and quantitative analysis of the amygdalin and 
      prunasin in plasma sample of rats which were administered by Maxing shigan 
      decoction.
FAU - Gao, Meng
AU  - Gao M
FAU - Wang, Yuesheng
AU  - Wang Y
FAU - Wei, Huizhen
AU  - Wei H
FAU - Ouyang, Hui
AU  - Ouyang H
FAU - He, Mingzhen
AU  - He M
FAU - Zeng, Lianqing
AU  - Zeng L
FAU - Shen, Fengyun
AU  - Shen F
FAU - Guo, Qiang
AU  - Guo Q
FAU - Rao, Yi
AU  - Rao Y
LA  - chi
PT  - Journal Article
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 0 (Nitriles)
RN  - 14W4BPM5FB (prunasin)
RN  - 214UUQ9N0H (Amygdalin)
SB  - IM
MH  - Amygdalin/*blood
MH  - Animals
MH  - Chromatography, High Pressure Liquid
MH  - Limit of Detection
MH  - Liquid-Liquid Extraction
MH  - Mass Spectrometry
MH  - Nitriles/*blood
MH  - Plasma/chemistry
MH  - Rats
MH  - Reproducibility of Results
EDAT- 2014/10/02 06:00
MHDA- 2015/09/02 06:00
CRDT- 2014/10/02 06:00
PHST- 2014/10/02 06:00 [entrez]
PHST- 2014/10/02 06:00 [pubmed]
PHST- 2015/09/02 06:00 [medline]
AID - 10.3724/sp.j.1123.2014.01021 [doi]
PST - ppublish
SO  - Se Pu. 2014 Jun;32(6):591-9. doi: 10.3724/sp.j.1123.2014.01021.