PMID- 25269265
OWN - NLM
STAT- MEDLINE
DCOM- 20150901
LR  - 20190923
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 32
IP  - 6
DP  - 2014 Jun
TI  - [Determination of six progesterones in milk by on-line cleanup liquid 
      chromatography-tandem mass spectrometry].
PG  - 647-52
AB  - A new method using TurboFlow on-line cleanup liquid chromatography combined with 
      tandem mass spectrometry (MS/MS) has been developed for simultaneous 
      determination of six progesterones including 19-norethindrone, 
      17alpha-hydroxyprogesterone, medroxyprogesterone, megestrol acetate, progesterone 
      and melengestrol acetate in milk. Samples were extracted by acetonitrile. The 
      analytes in extract were on-line purified directly on Cyclone-P purification 
      column where the sample matrices were washed away. Subsequently, the analytes 
      which were eluted from the extraction column onto Phenyl-Hexyl column were 
      separated with a gradient elution, and detected with electrospray ionization mass 
      spectrometry in the positive scan mode using multiple reaction monitoring (MRM). 
      The isotope internal standards were employed for quantification. As a result, the 
      linearities were satisfactory with the correlation coefficients of > 0.999 at 
      concentrations ranging from 0.1 microg/L to 50 microg/L. Based on the repeated 
      analysis of a known blank sample, the limit of quantification (LOQ) is 0.5 
      microg/kg. Average recoveries of the analytes fortified at three levels (1, 5 and 
      25 microg/kg) ranged from 90.8% to 107.5%, and the relative standard deviations 
      (RSDs) ranged from 6.3% to 11.8%. This proposed method is simple, rapid, 
      sensitive and highly selective, and can be applied to simultaneous identification 
      an quantification of the six progesterones in milk.
FAU - Duan, Yongsheng
AU  - Duan Y
FAU - Wang, Bingling
AU  - Wang B
FAU - Ai, Lianfeng
AU  - Ai L
FAU - Guo, Chunhai
AU  - Guo C
FAU - Ge, Shihui
AU  - Ge S
FAU - Zhang, Jingwen
AU  - Zhang J
FAU - Xu, Niusheng
AU  - Xu N
LA  - chi
PT  - Journal Article
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 4G7DS2Q64Y (Progesterone)
SB  - IM
MH  - Animals
MH  - Chromatography, Liquid
MH  - Milk/*chemistry
MH  - Progesterone/*analysis
MH  - Spectrometry, Mass, Electrospray Ionization
MH  - Tandem Mass Spectrometry
EDAT- 2014/10/02 06:00
MHDA- 2015/09/02 06:00
CRDT- 2014/10/02 06:00
PHST- 2014/10/02 06:00 [entrez]
PHST- 2014/10/02 06:00 [pubmed]
PHST- 2015/09/02 06:00 [medline]
AID - 10.3724/sp.j.1123.2014.01044 [doi]
PST - ppublish
SO  - Se Pu. 2014 Jun;32(6):647-52. doi: 10.3724/sp.j.1123.2014.01044.