PMID- 25269903 OWN - NLM STAT- MEDLINE DCOM- 20150409 LR - 20191210 IS - 2542-5641 (Electronic) IS - 0366-6999 (Linking) VI - 127 IP - 19 DP - 2014 TI - Neuroprotection via maintenance or increase of antioxidants and neurotrophic factors in ischemic gerbil hippocampus treated with tanshinone I. PG - 3396-405 AB - BACKGROUND: Danshen (Radix Salvia miltiorrhizae) has been used as a traditional medicine in Asia for treatment of various microcirculatory disturbance related diseases. Tanshinones are mainly hydrophobic active components, which have been isolated from Danshen and show various biological functions. In this study, we observed the neuroprotective effect of tanshinone I (TsI) against ischemic damage in the gerbil hippocampal CA1 region (CA1) after transient cerebral ischemia and examined its neuroprotective mechanism. METHODS: The gerbils were divided into vehicle-treated-sham-group, vehicle-treated-ischemia-group, TsI-treated-sham-group, and TsI-treated-ischemia-group. TsI was administrated intraperitoneally three times (once a day for three days) before ischemia-reperfusion. The neuroprotective effect of TsI was examined using H&E staining, neuronal nuclei (NeuN) immunohistochemistry and Fluoro-Jade B staining. To investigate the neuroprotective mechanism of TsI after ischemia-reperfusion, immunohistochemical (IHC) and Western blotting analyses for Cu, Zn-superoxide dismutase (SOD1), Mn-superoxide dismutase (SOD2), brain-derived neurotrophic factor (BDNF) and insulin-like growth factor-I (IGF-I) were performed. RESULTS: Treatment with TsI protected pyramidal neurons from ischemia-induced neuronal death in the CA1 after ischemia-reperfusion. In addition, treatment with TsI maintained the levels of SOD1 and SOD2 as determined by IHC and Western blotting in the CA1 after ischemia-reperfusion compared with the vehicle-ischemia-group. In addition, treatment with TsI increased the levels of BDNF and IGF-I determined by IHC and Western blotting in the TsI-treated-sham-group compared with the vehicle-treated-sham-group, and their levels were maintained in the stratum pyramidale of the ischemic CA1 in the TsI-treated-ischemia-group. CONCLUSION: Treatment with TsI protects pyramidal neurons of the CA1 from ischemic damage induced by transient cerebral ischemia via the maintenance of antioxidants and the increase of neurotrophic factors. FAU - Park, Joon Ha AU - Park JH AD - Department of Neurobiology, School of Medicine, Kangwon National University, Chuncheon 200-701, South Korea. FAU - Park, Ok Kyu AU - Park OK AD - Division of Analytical Bio-Imaging, Chuncheon Center, Korea Basic Science Institute, Chuncheon 200-701, South Korea. FAU - Yan, Bingchun AU - Yan B AD - Department of Integrative Traditional & Western Medicine, Medical College, Yangzhou University, Yangzhou, Jiangsu 225001, China. FAU - Ahn, Ji Hyeon AU - Ahn JH AD - Department of Neurobiology, School of Medicine, Kangwon National University, Chuncheon 200-701, South Korea. FAU - Kim, In Hye AU - Kim IH AD - Department of Neurobiology, School of Medicine, Kangwon National University, Chuncheon 200-701, South Korea. FAU - Lee, Jae-Chul AU - Lee JC AD - Department of Neurobiology, School of Medicine, Kangwon National University, Chuncheon 200-701, South Korea. FAU - Kwon, Seung-Hae AU - Kwon SH AD - Division of Analytical Bio-Imaging, Chuncheon Center, Korea Basic Science Institute, Chuncheon 200-701, South Korea. FAU - Yoo, Ki-Yeon AU - Yoo KY AD - Department of Oral Anatomy, College of Dentistry, Gangneung-Wonju National University, Gangneung 210-702, South Korea. FAU - Lee, Choong Hyun AU - Lee CH AD - Department of Pharmacy, College of Pharmacy, Dankook University, Cheonan 330-714, South Korea. FAU - Hwang, In Koo AU - Hwang IK AD - Department of Anatomy and Cell Biology, College of Veterinary Medicine, and Research Institute for Veterinary Science, Seoul National University, Seoul 151-742, South Korea. FAU - Choi, Jung Hoon AU - Choi JH AD - Department of Anatomy, College of Veterinary Medicine, Kangwon National University, Chuncheon 200-701, South Korea. FAU - Won, Moo-Ho AU - Won MH AD - Department of Neurobiology, School of Medicine, Kangwon National University, Chuncheon 200-701, South Korea. Email: mhwon@kangwon.ac.kr. FAU - Kim, Jong-Dai AU - Kim JD AD - Division of Food Science and Biotechnology, College of Agriculture and Life Sciences, Kangwon National University, Chuncheon 200-701, South Korea. Email: jongdai@cc.kangwon.ac.kr. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - China TA - Chin Med J (Engl) JT - Chinese medical journal JID - 7513795 RN - 0 (Abietanes) RN - 0 (Antioxidants) RN - 0 (Brain-Derived Neurotrophic Factor) RN - 0 (Nerve Growth Factors) RN - 03UUH3J385 (tanshinone) RN - 67763-96-6 (Insulin-Like Growth Factor I) RN - EC 1.15.1.1 (Superoxide Dismutase) RN - EC 1.15.1.1 (Superoxide Dismutase-1) RN - EC 1.15.1.1 (superoxide dismutase 2) SB - IM MH - Abietanes/*therapeutic use MH - Animals MH - Antioxidants/*metabolism MH - Blotting, Western MH - Brain Ischemia/*drug therapy/*metabolism MH - Brain-Derived Neurotrophic Factor/metabolism MH - Gerbillinae MH - Hippocampus/*metabolism MH - Immunohistochemistry MH - Insulin-Like Growth Factor I/metabolism MH - Male MH - Nerve Growth Factors/*metabolism MH - Superoxide Dismutase/metabolism MH - Superoxide Dismutase-1 EDAT- 2014/10/02 06:00 MHDA- 2015/04/10 06:00 CRDT- 2014/10/02 06:00 PHST- 2014/10/02 06:00 [entrez] PHST- 2014/10/02 06:00 [pubmed] PHST- 2015/04/10 06:00 [medline] PST - ppublish SO - Chin Med J (Engl). 2014;127(19):3396-405.