PMID- 25270527
OWN - NLM
STAT- MEDLINE
DCOM- 20160119
LR  - 20231213
IS  - 1365-2559 (Electronic)
IS  - 0309-0167 (Linking)
VI  - 66
IP  - 6
DP  - 2015 May
TI  - Down-regulation of connexin43 and connexin32 in keratocystic odontogenic tumours: 
      potential association with clinical features.
PG  - 798-807
LID - 10.1111/his.12569 [doi]
AB  - AIMS: The objective of this study was to explore the potential involvement of 
      connexin43 (Cx43) and connexin32 (Cx32), two vital members of the connexin 
      families, in the pathogenesis of keratocystic odontogenic tumours (KCOT). METHODS 
      AND RESULTS: The expression levels of Cx43 and Cx32 in human KCOT and normal oral 
      mucosa (OM) tissues were measured using immunohistochemistry and real-time 
      quantitative polymerase chain reaction (qPCR). The relationship between Cx43 and 
      Cx32 expression and markers of proliferation [proliferating cell nuclear antigen 
      (PCNA), cyclin D1], anti-apoptosis [B cell lymphoma 2 (Bcl-2)] and autophagy 
      [light chain 3 (LC3), Sequestosome 1 p62 (p62)] was then investigated in the KCOT 
      samples. The results showed that Cx43 and Cx32 expression was down-regulated 
      significantly in KCOT samples relative to OM samples. Meanwhile, the expression 
      levels of Cx43 and Cx32 were correlated negatively with the expression levels of 
      PCNA, cyclin D1, Bcl-2, LC3 and p62, as confirmed further by double-labelling 
      immunofluorescence analyses. CONCLUSIONS: This study reveals for the first time 
      that Cx43 and Cx32 are down-regulated in KCOT and suggests an association with 
      growth regulation, anti-apoptosis and autophagy in KCOT.
CI  - (c) 2014 John Wiley & Sons Ltd.
FAU - Zhong, Wen-Qun
AU  - Zhong WQ
AD  - State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) 
      and Key Laboratory of Oral Biomedicine Ministry of Education, School and Hospital 
      of Stomatology, Wuhan University, Wuhan, China.
FAU - Chen, Gang
AU  - Chen G
FAU - Zhang, Wei
AU  - Zhang W
FAU - Xiong, Xue-Peng
AU  - Xiong XP
FAU - Ren, Jian-Gang
AU  - Ren JG
FAU - Zhao, Yi
AU  - Zhao Y
FAU - Liu, Bing
AU  - Liu B
FAU - Zhao, Yi-Fang
AU  - Zhao YF
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150128
PL  - England
TA  - Histopathology
JT  - Histopathology
JID - 7704136
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (Connexin 43)
RN  - 0 (Connexins)
RN  - 0 (GJA1 protein, human)
SB  - IM
MH  - Apoptosis/physiology
MH  - Autophagy/physiology
MH  - Biomarkers, Tumor/analysis/*biosynthesis
MH  - Cluster Analysis
MH  - Connexin 43/analysis/*biosynthesis
MH  - Connexins/analysis/*biosynthesis
MH  - Down-Regulation
MH  - Humans
MH  - Immunohistochemistry
MH  - Odontogenic Cysts/metabolism/*pathology
MH  - Odontogenic Tumors/metabolism/*pathology
MH  - Real-Time Polymerase Chain Reaction
MH  - Gap Junction beta-1 Protein
OTO - NOTNLM
OT  - apoptosis
OT  - autophagy
OT  - connexin
OT  - keratocystic odontogenic tumour
OT  - proliferation
EDAT- 2014/10/02 06:00
MHDA- 2016/01/20 06:00
CRDT- 2014/10/02 06:00
PHST- 2014/06/10 00:00 [received]
PHST- 2014/09/26 00:00 [accepted]
PHST- 2014/10/02 06:00 [entrez]
PHST- 2014/10/02 06:00 [pubmed]
PHST- 2016/01/20 06:00 [medline]
AID - 10.1111/his.12569 [doi]
PST - ppublish
SO  - Histopathology. 2015 May;66(6):798-807. doi: 10.1111/his.12569. Epub 2015 Jan 28.