PMID- 25270759
OWN - NLM
STAT- MEDLINE
DCOM- 20151102
LR  - 20240321
IS  - 1471-2229 (Electronic)
IS  - 1471-2229 (Linking)
VI  - 14
DP  - 2014 Oct 1
TI  - Phosphite-induced changes of the transcriptome and secretome in Solanum tuberosum 
      leading to resistance against Phytophthora infestans.
PG  - 254
LID - 10.1186/s12870-014-0254-y [doi]
LID - 254
AB  - BACKGROUND: Potato late blight caused by the oomycete pathogen Phytophthora 
      infestans can lead to immense yield loss. We investigated the transcriptome of 
      Solanum tubersoum (cv. Desiree) and characterized the secretome by quantitative 
      proteomics after foliar application of the protective agent phosphite. We also 
      studied the distribution of phosphite in planta after application and tested 
      transgenic potato lines with impaired in salicylic and jasmonic acid signaling. 
      RESULTS: Phosphite had a rapid and transient effect on the transcriptome, with a 
      clear response 3 h after treatment. Strikingly this effect lasted less than 24 h, 
      whereas protection was observed throughout all time points tested. In contrast, 
      67 secretome proteins predominantly associated with cell-wall processes and 
      defense changed in abundance at 48 h after treatment. Transcripts associated with 
      defense, wounding, and oxidative stress constituted the core of the phosphite 
      response. We also observed changes in primary metabolism and cell wall-related 
      processes. These changes were shown not to be due to phosphate depletion or 
      acidification caused by phosphite treatment. Of the phosphite-regulated 
      transcripts 40% also changed with beta-aminobutyric acid (BABA) as an elicitor, 
      while the defence gene PR1 was only up-regulated by BABA. Although phosphite was 
      shown to be distributed in planta to parts not directly exposed to phosphite, no 
      protection in leaves without direct foliar application was observed. Furthermore, 
      the analysis of transgenic potato lines indicated that the phosphite-mediated 
      resistance was independent of the plant hormones salicylic and jasmonic acid. 
      CONCLUSIONS: Our study suggests that a rapid phosphite-triggered response is 
      important to confer long-lasting resistance against P. infestans and gives 
      molecular understanding of its successful field applications.
FAU - Burra, Dharani Dhar
AU  - Burra DD
FAU - Berkowitz, Oliver
AU  - Berkowitz O
FAU - Hedley, Pete E
AU  - Hedley PE
FAU - Morris, Jenny
AU  - Morris J
FAU - Resjo, Svante
AU  - Resjo S
FAU - Levander, Fredrik
AU  - Levander F
FAU - Liljeroth, Erland
AU  - Liljeroth E
FAU - Andreasson, Erik
AU  - Andreasson E
FAU - Alexandersson, Erik
AU  - Alexandersson E
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20141001
PL  - England
TA  - BMC Plant Biol
JT  - BMC plant biology
JID - 100967807
RN  - 0 (Aminobutyrates)
RN  - 0 (Phosphites)
RN  - 0 (Plant Proteins)
RN  - 4282SA5CTS (3-aminobutyric acid)
SB  - IM
MH  - Aminobutyrates/pharmacology
MH  - Gene Expression Regulation, Plant/*drug effects
MH  - Gene Ontology
MH  - Phosphites/analysis/*pharmacology
MH  - Phytophthora infestans/*physiology
MH  - Plant Diseases/*immunology
MH  - Plant Immunity
MH  - Plant Leaves/drug effects/genetics/immunology
MH  - Plant Proteins/genetics/metabolism
MH  - Plants, Genetically Modified
MH  - Solanum tuberosum/*drug effects/genetics/immunology
MH  - *Transcriptome
PMC - PMC4192290
EDAT- 2014/10/02 06:00
MHDA- 2015/11/03 06:00
PMCR- 2014/10/01
CRDT- 2014/10/02 06:00
PHST- 2014/06/03 00:00 [received]
PHST- 2014/09/20 00:00 [accepted]
PHST- 2014/10/02 06:00 [entrez]
PHST- 2014/10/02 06:00 [pubmed]
PHST- 2015/11/03 06:00 [medline]
PHST- 2014/10/01 00:00 [pmc-release]
AID - s12870-014-0254-y [pii]
AID - 254 [pii]
AID - 10.1186/s12870-014-0254-y [doi]
PST - epublish
SO  - BMC Plant Biol. 2014 Oct 1;14:254. doi: 10.1186/s12870-014-0254-y.