PMID- 25274952
OWN - NLM
STAT- MEDLINE
DCOM- 20160105
LR  - 20161018
IS  - 1437-4331 (Electronic)
IS  - 1434-6621 (Linking)
VI  - 53
IP  - 5
DP  - 2015 Apr
TI  - Diagnostic performance study of an antigen microarray for the detection of 
      antiphospholipid antibodies in human serum.
PG  - 801-8
LID - 10.1515/cclm-2014-0569 [doi]
AB  - BACKGROUND: The parallelization of clinically relevant antigens in a microarray 
      format is of growing importance due to the ability to measure multiple 
      antigen-antibody interactions. With the development of a microarray for the 
      detection of antiphospholipid antibodies we focussed on one important autoimmune 
      disease that is still diagnostically challenging. Reasons are the heterogeneity 
      of the autoantibodies and the unspecific clinical symptoms. METHODS: For the 
      covalent immobilization of antigenic structures, glass transducers were coated 
      with 11-aminoundecyltrimethoxysilane (11-AUTMS). In total 35 antiphospholipid 
      syndrome (APS) patients, six patients with lupus erythematosus and 24 healthy 
      controls were investigated on a microarray format using polarized imaging 
      reflectometric interference spectroscopy. RESULTS: The novel surface modification 
      based on the short derivative 11-AUTMS resulted in a selective biosensor allowing 
      a clear differentiation of patient and control samples. It combined proteinogenic 
      as well as phospholipid-derived antigens, namely beta2-glycoprotein I (beta2-GPI), 
      prothrombin, cardiolipin (CL) and a beta2-GPI/CL complex. With optimized 
      regeneration conditions, up to 20 consecutive measurements could be performed on 
      one chip. Sensitivity was determined to be 0.800-0.929, specificity was between 
      0.733 and 0.969, depending on the respective antigen. CONCLUSIONS: Multiplexed 
      determination of serological parameters has a great potential. We have shown that 
      our biosensor is capable of detecting four different APS relevant antibodies in 
      parallel exhibiting a sensitivity and specificity comparable to existing ELISA 
      methods.
FAU - Schindler, Aline R
AU  - Schindler AR
FAU - Bleher, Oliver
AU  - Bleher O
FAU - Thaler, Markus A
AU  - Thaler MA
FAU - Kocot, Carmen J
AU  - Kocot CJ
FAU - Steigerwald, Udo
AU  - Steigerwald U
FAU - Proll, Gunther
AU  - Proll G
FAU - Gauglitz, Gunter
AU  - Gauglitz G
FAU - Luppa, Peter B
AU  - Luppa PB
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Clin Chem Lab Med
JT  - Clinical chemistry and laboratory medicine
JID - 9806306
RN  - 0 (Antibodies, Antiphospholipid)
RN  - 0 (Antigens)
RN  - 0 (Immobilized Proteins)
RN  - 0 (beta 2-Glycoprotein I)
SB  - IM
MH  - Antibodies, Antiphospholipid/*analysis/immunology
MH  - Antigens/chemistry/*immunology
MH  - Antiphospholipid Syndrome/*diagnosis
MH  - Glass/chemistry
MH  - Humans
MH  - Immobilized Proteins/chemistry/immunology
MH  - Microarray Analysis/*methods
MH  - beta 2-Glycoprotein I/chemistry/immunology
EDAT- 2014/10/03 06:00
MHDA- 2016/01/06 06:00
CRDT- 2014/10/03 06:00
PHST- 2014/05/28 00:00 [received]
PHST- 2014/08/25 00:00 [accepted]
PHST- 2014/10/03 06:00 [entrez]
PHST- 2014/10/03 06:00 [pubmed]
PHST- 2016/01/06 06:00 [medline]
AID - /j/cclm.ahead-of-print/cclm-2014-0569/cclm-2014-0569.xml [pii]
AID - 10.1515/cclm-2014-0569 [doi]
PST - ppublish
SO  - Clin Chem Lab Med. 2015 Apr;53(5):801-8. doi: 10.1515/cclm-2014-0569.