PMID- 25276110
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20141003
LR  - 20211021
IS  - 1931-7573 (Print)
IS  - 1556-276X (Electronic)
IS  - 1556-276X (Linking)
VI  - 9
IP  - 1
DP  - 2014
TI  - AHAPS-functionalized silica nanoparticles do not modulate allergic contact 
      dermatitis in mice.
PG  - 524
LID - 10.1186/1556-276X-9-524 [doi]
AB  - Allergic contact dermatitis (ACD) is a common skin disease in people and may 
      become a potential site of exposure to nanoparticles (NP). Silica nanoparticles 
      (SiO2-NP) possess a promising potential for various medical and non-medical 
      applications, including normal and diseased skin as target organs. However, it 
      has been shown that negatively charged SiO2-NP may act as proinflammatory 
      adjuvant in allergic diseases. The effect of topical SiO2-NP exposure on 
      preexisting ACD has not been studied to date although this reflects a common in 
      vivo situation. Of particular interest are the potential effects of positively 
      charged N-(6-aminohexyl)-aminopropyltrimethoxysilane (AHAPS)-functionalized 
      SiO2-NP which are promising candidates for delivery systems, including gene 
      delivery into the skin. Here, the effects of such AHAPS-functionalized SiO2-NP 
      (55 +/- 6 nm in diameter) were studied in an oxazolone-induced ACD model in SKH1 
      mice and compared to ACD mice treated with vehicle only. The clinical course of 
      the disease was assessed by monitoring of the transepidermal water loss (TEWL) 
      and the erythema. In histologic and morphometric analyses, the distribution of 
      particles, the degree of inflammation, epidermal thickness, and the inflammatory 
      infiltrate were characterized and quantified by standard and special histological 
      stains as well as immunohistochemistry for CD3+ lymphocytes. To assess possible 
      systemic effects, serum immunoglobulin E (IgE) was determined by enzyme-linked 
      immunosorbent assay. Following administration of AHAPS-SiO2-NP for five 
      consecutive days, no effects were observed in all clinical, histologic, 
      morphometric, and molecular parameters investigated. In conclusion, positively 
      charged AHAPS-SiO2-NP seem not to affect the course of ACD during exposure for 
      5 days.
FAU - Ostrowski, Anja
AU  - Ostrowski A
AD  - Institute of Veterinary Pathology, Freie Universitat Berlin, 
      Robert-von-Ostertag-Str. 15, 14163 Berlin, Germany.
FAU - Nordmeyer, Daniel
AU  - Nordmeyer D
AD  - Institute of Chemistry and Biochemistry - Physical and Theoretical Chemistry, 
      Freie Universitat Berlin, Takustr. 3, 14195 Berlin, Germany.
FAU - Mundhenk, Lars
AU  - Mundhenk L
AD  - Institute of Veterinary Pathology, Freie Universitat Berlin, 
      Robert-von-Ostertag-Str. 15, 14163 Berlin, Germany.
FAU - Fluhr, Joachim W
AU  - Fluhr JW
AD  - Department of Dermatology, Venerology and Allergology, Charite - 
      Universitatsmedizin Berlin, Chariteplatz 1, 10117 Berlin, Germany.
FAU - Lademann, Jurgen
AU  - Lademann J
AD  - Department of Dermatology, Venerology and Allergology, Charite - 
      Universitatsmedizin Berlin, Chariteplatz 1, 10117 Berlin, Germany.
FAU - Graf, Christina
AU  - Graf C
AD  - Institute of Chemistry and Biochemistry - Physical and Theoretical Chemistry, 
      Freie Universitat Berlin, Takustr. 3, 14195 Berlin, Germany.
FAU - Ruhl, Eckart
AU  - Ruhl E
AD  - Institute of Chemistry and Biochemistry - Physical and Theoretical Chemistry, 
      Freie Universitat Berlin, Takustr. 3, 14195 Berlin, Germany.
FAU - Gruber, Achim D
AU  - Gruber AD
AD  - Institute of Veterinary Pathology, Freie Universitat Berlin, 
      Robert-von-Ostertag-Str. 15, 14163 Berlin, Germany.
LA  - eng
PT  - Journal Article
DEP - 20140924
PL  - United States
TA  - Nanoscale Res Lett
JT  - Nanoscale research letters
JID - 101279750
PMC - PMC4177380
OTO - NOTNLM
OT  - Allergic contact dermatitis
OT  - Mouse model
OT  - Oxazolone
OT  - Silica nanoparticles
OT  - Toxicopathology
EDAT- 2014/10/03 06:00
MHDA- 2014/10/03 06:01
PMCR- 2014/09/24
CRDT- 2014/10/03 06:00
PHST- 2014/07/03 00:00 [received]
PHST- 2014/09/16 00:00 [accepted]
PHST- 2014/10/03 06:00 [entrez]
PHST- 2014/10/03 06:00 [pubmed]
PHST- 2014/10/03 06:01 [medline]
PHST- 2014/09/24 00:00 [pmc-release]
AID - 1556-276X-9-524 [pii]
AID - 10.1186/1556-276X-9-524 [doi]
PST - epublish
SO  - Nanoscale Res Lett. 2014 Sep 24;9(1):524. doi: 10.1186/1556-276X-9-524. 
      eCollection 2014.