PMID- 25279238
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20141003
LR  - 20211021
IS  - 2093-4777 (Print)
IS  - 2093-6931 (Electronic)
IS  - 2093-4777 (Linking)
VI  - 18
IP  - 3
DP  - 2014 Sep
TI  - Inhibitory Effects of Isoquinoline Alkaloid Berberine on Ischemia-Induced 
      Apoptosis via Activation of Phosphoinositide 3-Kinase/Protein Kinase B Signaling 
      Pathway.
PG  - 115-25
LID - 10.5213/inj.2014.18.3.115 [doi]
AB  - PURPOSE: Berberine is a type of isoquinoline alkaloid that has been used to treat 
      various diseases. A neuroprotective effect of berberine against cerebral ischemia 
      has been reported; however, the effects of berberine on apoptosis in relation to 
      reactive astrogliosis and microglia activation under ischemic conditions have not 
      yet been fully evaluated. In the present study, we investigated the effects of 
      berberine on global ischemia-induced apoptosis, and focused on the 
      phosphoinositide 3-kinase (PI3K)/protein kinase B (Akt) signaling pathway in the 
      hippocampus using gerbils. METHODS: Gerbils received berberine orally once a day 
      for 14 consecutive days, starting one day after surgery. In this study, a 
      step-down avoidance task was used to assess short-term memory. Furthermore, we 
      employed the terminal deoxynucleotidyl transferase-mediated dUTP nick end 
      labeling (TUNEL) assay to evaluate DNA fragmentation, immunohistochemistry to 
      investigate glial fibriallary acidic protein, CD11b, and caspase-3, and western 
      blot to assess PI3K, Akt, Bax, Bcl-2, and cytochrome c. RESULTS: Our results 
      revealed that berberine treatment alleviated ischemia-induced short-term memory 
      impairment. Treatment with berbeine also attenuated ischemia-induced apoptosis 
      and inhibited reactive astrogliosis and microglia activation. Furthermore, 
      berberine enhanced phospho-PI3K and phospho-Akt expression in the hippocampus of 
      ischemic gerbils. CONCLUSIONS: Berberine exerted a neuroprotective effect against 
      ischemic insult by inhibiting neuronal apoptosis via activation of the PI3K/Akt 
      signaling pathway. The antiapoptotic effect of berberine was achieved through 
      inhibition of reactive astrogliosis and microglia activation. Berberine may 
      therefore serve as a therapeutic agent for stroke-induced neurourological 
      problems.
FAU - Kim, Mia
AU  - Kim M
AD  - Department of Cardiovascular and Neurologic Diseases (Stroke Center), College of 
      Oriental Medicine, Kyung Hee University, Seoul, Korea.
FAU - Shin, Mal Soon
AU  - Shin MS
AD  - Department of Physiology, Kyung Hee University School of Medicine, Seoul, Korea.
FAU - Lee, Jae Min
AU  - Lee JM
AD  - Department of Physiology, Kyung Hee University School of Medicine, Seoul, Korea.
FAU - Cho, Han Sam
AU  - Cho HS
AD  - Department of Physiology, Kyung Hee University School of Medicine, Seoul, Korea.
FAU - Kim, Chang Ju
AU  - Kim CJ
AD  - Department of Physiology, Kyung Hee University School of Medicine, Seoul, Korea.
FAU - Kim, Young Joon
AU  - Kim YJ
AD  - Department of Biochemistry and Molecular Biology, Kyung Hee University School of 
      Medicine, Seoul, Korea.
FAU - Choi, Hey Ran
AU  - Choi HR
AD  - Department of Biochemistry and Molecular Biology, Kyung Hee University School of 
      Medicine, Seoul, Korea.
FAU - Jeon, Jung Won
AU  - Jeon JW
AD  - Department of Internal Medicine, Kyung Hee University Hospital at Gangdong, Kyung 
      Hee University School of Medicine, Seoul, Korea.
LA  - eng
PT  - Journal Article
DEP - 20140924
PL  - Korea (South)
TA  - Int Neurourol J
JT  - International neurourology journal
JID - 101534513
PMC - PMC4180161
OTO - NOTNLM
OT  - Apoptosis
OT  - Berberine
OT  - Brain ischemia
OT  - Phosphatidylinositol 3-kinases
OT  - Short-term memory
COIS- The authors report no potential conflict of interest relevant to this article.
EDAT- 2014/10/04 06:00
MHDA- 2014/10/04 06:01
PMCR- 2014/09/01
CRDT- 2014/10/04 06:00
PHST- 2014/09/09 00:00 [received]
PHST- 2014/09/17 00:00 [accepted]
PHST- 2014/10/04 06:00 [entrez]
PHST- 2014/10/04 06:00 [pubmed]
PHST- 2014/10/04 06:01 [medline]
PHST- 2014/09/01 00:00 [pmc-release]
AID - 10.5213/inj.2014.18.3.115 [doi]
PST - ppublish
SO  - Int Neurourol J. 2014 Sep;18(3):115-25. doi: 10.5213/inj.2014.18.3.115. Epub 2014 
      Sep 24.