PMID- 25280105
OWN - NLM
STAT- MEDLINE
DCOM- 20141229
LR  - 20141004
IS  - 1097-4164 (Electronic)
IS  - 1097-2765 (Linking)
VI  - 56
IP  - 1
DP  - 2014 Oct 2
TI  - MicroRNAs trigger dissociation of eIF4AI and eIF4AII from target mRNAs in humans.
PG  - 79-89
LID - S1097-2765(14)00712-6 [pii]
LID - 10.1016/j.molcel.2014.09.005 [doi]
AB  - In animals, key functions of microRNA-induced silencing complex (miRISC) are 
      translational repression and deadenylation followed by mRNA decay. While miRISC 
      represses translation initiation, it is poorly understood how miRISC exerts this 
      function. Here we assessed the effect of miRISC on synergistic recruitment of 
      translation initiation factors to target mRNAs by using direct biochemical 
      assays. We show that miRISC promotes eIF4AI and eIF4AII release from target mRNAs 
      prior to dissociation of eIF4E and eIF4G in a deadenylation-independent manner. 
      Strikingly, miRISC-induced release of eIF4AI and eIF4AII from target mRNAs and 
      miRISC-induced inhibition of cap-dependent translation can both be counteracted 
      by the RNA-binding protein HuD via a direct interaction of HuD with eIF4A. 
      Furthermore, the pharmacological eIF4A inhibitor silvestrol, which locks eIF4A on 
      mRNAs, conferred resistance to miRNA-mediated translational repression. In 
      summary, we propose that both eIF4AI and eIF4AII are functionally important 
      targets in miRISC-mediated translation control.
CI  - Copyright (c) 2014 Elsevier Inc. All rights reserved.
FAU - Fukao, Akira
AU  - Fukao A
AD  - Laboratory of Hygienic Chemistry, Graduate School of Pharmaceutical Sciences, 
      Nagoya City University, 3-1 Tanabe-dori, Mizuho-ku, Nagoya 467-8603, Japan.
FAU - Mishima, Yuichiro
AU  - Mishima Y
AD  - Institute of Molecular and Cellular Biosciences, Department of Medical Genome 
      Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-0032, Japan.
FAU - Takizawa, Naoki
AU  - Takizawa N
AD  - Institute of Microbial Chemistry, Laboratory of Basic Biology, 3-14-23 Kamiosaki, 
      Shinagawa-ku, Tokyo 141-0021, Japan.
FAU - Oka, Shigenori
AU  - Oka S
AD  - Pharma Medical Division, Life & Healthcare Products Department, Nagase & Co., 
      Ltd., 2-2-3 Murotani, Nishi-ku, Kobe, Hyogo 651-2241, Japan.
FAU - Imataka, Hiroaki
AU  - Imataka H
AD  - Department of Materials Science and Chemistry, Graduate School of Engineering, 
      University of Hyogo, Himeji 671-2280, Japan.
FAU - Pelletier, Jerry
AU  - Pelletier J
AD  - Department of Biochemistry, Department of Oncology, and The Rosalind and Morris 
      Goodman Cancer Research Center and McGill Cancer Centre, McGill University, 
      Montreal, QC H3G 1Y6, Canada.
FAU - Sonenberg, Nahum
AU  - Sonenberg N
AD  - Department of Biochemistry, Department of Oncology, and The Rosalind and Morris 
      Goodman Cancer Research Center and McGill Cancer Centre, McGill University, 
      Montreal, QC H3G 1Y6, Canada.
FAU - Thoma, Christian
AU  - Thoma C
AD  - Department of Medicine II, University Hospital of Freiburg, Hugstetterstr. 55, 
      79106 Freiburg, Germany.
FAU - Fujiwara, Toshinobu
AU  - Fujiwara T
AD  - Laboratory of Hygienic Chemistry, Graduate School of Pharmaceutical Sciences, 
      Nagoya City University, 3-1 Tanabe-dori, Mizuho-ku, Nagoya 467-8603, Japan. 
      Electronic address: tosinobu@phar.nagoya-cu.ac.jp.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Mol Cell
JT  - Molecular cell
JID - 9802571
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA-Induced Silencing Complex)
RN  - 0 (Triterpenes)
RN  - 0 (silvestrol)
RN  - EC 2.7.7.- (Eukaryotic Initiation Factor-4A)
SB  - IM
MH  - Eukaryotic Initiation Factor-4A/antagonists & inhibitors/genetics/*metabolism
MH  - HEK293 Cells
MH  - Humans
MH  - MicroRNAs/*physiology
MH  - *Models, Genetic
MH  - RNA, Messenger/*metabolism
MH  - RNA-Induced Silencing Complex/physiology
MH  - Transcription Initiation, Genetic
MH  - Triterpenes/pharmacology
EDAT- 2014/10/04 06:00
MHDA- 2014/12/30 06:00
CRDT- 2014/10/04 06:00
PHST- 2014/01/31 00:00 [received]
PHST- 2014/07/18 00:00 [revised]
PHST- 2014/08/28 00:00 [accepted]
PHST- 2014/10/04 06:00 [entrez]
PHST- 2014/10/04 06:00 [pubmed]
PHST- 2014/12/30 06:00 [medline]
AID - S1097-2765(14)00712-6 [pii]
AID - 10.1016/j.molcel.2014.09.005 [doi]
PST - ppublish
SO  - Mol Cell. 2014 Oct 2;56(1):79-89. doi: 10.1016/j.molcel.2014.09.005.