PMID- 25280681
OWN - NLM
STAT- MEDLINE
DCOM- 20150616
LR  - 20141004
IS  - 1873-0191 (Electronic)
IS  - 0928-4931 (Linking)
VI  - 44
DP  - 2014 Nov
TI  - Molecularly imprinted solid-phase extraction of glutathione from urine samples.
PG  - 69-75
LID - S0928-4931(14)00490-1 [pii]
LID - 10.1016/j.msec.2014.08.005 [doi]
AB  - Molecularly imprinted polymer (MIP) particles for glutathione were synthesized 
      through iniferter-controlled living radical precipitation polymerization (IRPP) 
      under ultraviolet radiation at ambient temperature. Static adsorption, 
      solid-phase extraction, and high-performance liquid chromatography were carried 
      out to evaluate the adsorption properties and selective recognition 
      characteristics of the polymers for glutathione and its structural analogs. The 
      obtained IRPP-MIP particles exhibited a regularly spherical shape, rapid binding 
      kinetics, high imprinting factor, and high selectivity compared with the MIP 
      particles prepared using traditional free-radical precipitation polymerization. 
      The selective separation and enrichment of glutathione from the mixture of 
      glycyl-glycine and glutathione disulfide could be achieved on the IRPP-MIP 
      cartridge. The recoveries of glutathione, glycyl-glycine, and glutathione 
      disulfide were 95.6% +/- 3.65%, 29.5% +/- 1.26%, and 49.9% +/- 1.71%, respectively. The 
      detection limit (S/N=3) of glutathione was 0.5 mg.L(-1). The relative standard 
      deviations (RSDs) for 10 replicate detections of 50 mg.L(-1) of glutathione were 
      5.76%, and the linear range of the calibration curve was 0.5 mg.L(-1) to 200 
      mg.L(-1) under optimized conditions. The proposed approach was successfully 
      applied to determine glutathione in spiked human urine samples with recoveries of 
      90.24% to 96.20% and RSDs of 0.48% to 5.67%.
CI  - Copyright (c) 2014 Elsevier B.V. All rights reserved.
FAU - Song, Renyuan
AU  - Song R
AD  - School of Natural and Applied Science, Northwestern Polytechnical University, 
      Xi'an 710072, PR China. Electronic address: songrenyuan0726@163.com.
FAU - Hu, Xiaoling
AU  - Hu X
AD  - School of Natural and Applied Science, Northwestern Polytechnical University, 
      Xi'an 710072, PR China.
FAU - Guan, Ping
AU  - Guan P
AD  - School of Natural and Applied Science, Northwestern Polytechnical University, 
      Xi'an 710072, PR China.
FAU - Li, Ji
AU  - Li J
AD  - School of Natural and Applied Science, Northwestern Polytechnical University, 
      Xi'an 710072, PR China.
FAU - Zhao, Na
AU  - Zhao N
AD  - School of Natural and Applied Science, Northwestern Polytechnical University, 
      Xi'an 710072, PR China.
FAU - Wang, Qiaoli
AU  - Wang Q
AD  - School of Natural and Applied Science, Northwestern Polytechnical University, 
      Xi'an 710072, PR China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140807
PL  - Netherlands
TA  - Mater Sci Eng C Mater Biol Appl
JT  - Materials science & engineering. C, Materials for biological applications
JID - 101484109
RN  - 0 (Polymers)
RN  - GAN16C9B8O (Glutathione)
SB  - IM
MH  - Adsorption
MH  - Chromatography, High Pressure Liquid
MH  - Glutathione/*urine
MH  - Humans
MH  - Hydrogen-Ion Concentration
MH  - Molecular Imprinting
MH  - Molecular Structure
MH  - Polymerization
MH  - Polymers/chemistry
MH  - *Solid Phase Extraction
MH  - Ultraviolet Rays
OTO - NOTNLM
OT  - Glutathione
OT  - Living radical polymerization
OT  - Molecularly imprinted polymer
OT  - Selectivity extraction
OT  - Solid-phase extraction
EDAT- 2014/10/05 06:00
MHDA- 2015/06/17 06:00
CRDT- 2014/10/05 06:00
PHST- 2013/12/05 00:00 [received]
PHST- 2014/06/10 00:00 [revised]
PHST- 2014/08/01 00:00 [accepted]
PHST- 2014/10/05 06:00 [entrez]
PHST- 2014/10/05 06:00 [pubmed]
PHST- 2015/06/17 06:00 [medline]
AID - S0928-4931(14)00490-1 [pii]
AID - 10.1016/j.msec.2014.08.005 [doi]
PST - ppublish
SO  - Mater Sci Eng C Mater Biol Appl. 2014 Nov;44:69-75. doi: 
      10.1016/j.msec.2014.08.005. Epub 2014 Aug 7.