PMID- 25280726
OWN - NLM
STAT- MEDLINE
DCOM- 20150616
LR  - 20141004
IS  - 1873-0191 (Electronic)
IS  - 0928-4931 (Linking)
VI  - 44
DP  - 2014 Nov
TI  - Enhancement of skin wound healing with decellularized scaffolds loaded with 
      hyaluronic acid and epidermal growth factor.
PG  - 440-8
LID - S0928-4931(14)00446-9 [pii]
LID - 10.1016/j.msec.2014.07.039 [doi]
AB  - Current therapy for skin wound healing still relies on skin transplantation. Many 
      studies were done to try to find out ways to replace skin transplantation, but 
      there is still no effective alternative therapy. In this study, decellularized 
      scaffolds were prepared from pig peritoneum by a series of physical and chemical 
      treatments, and scaffolds loaded with hyaluronic acid (HA) and epidermal growth 
      factor (EGF) were tested for their effect on wound healing. MTT assay showed that 
      EGF increased NIH3T3 cell viability and confirmed that EGF used in this study was 
      biologically active in vitro. Scanning electron microscope (SEM) showed that HA 
      stably attached to scaffolds even after soaking in PBS for 48 h. ELISA assay 
      showed that HA increased the adsorption of EGF to scaffolds and sustained the 
      release of EGF from scaffolds. Animal study showed that the wounds covered with 
      scaffolds containing HA and EGF recovered best among all 4 groups and had wound 
      healing rates of 49.86%, 70.94% and 87.41% respectively for days 10, 15 and 20 
      post-surgery compared to scaffolds alone with wound healing rates of 29.26%, 
      42.80% and 70.14%. In addition, the wounds covered with scaffolds containing EGF 
      alone were smaller than no EGF scaffolds on days 10, 15 and 20 post-surgery. 
      Hematoxylin-Eosin (HE) staining confirmed these results by showing that on days 
      10, 15 and 20 post-surgery, the thicker epidermis and dermis layers were observed 
      in the wounds covered with scaffolds containing HA and EGF than scaffolds alone. 
      In addition, the thicker epidermis and dermis layers were also observed in the 
      wounds covered with scaffolds containing EGF than scaffolds alone. Skin 
      appendages were observed on day 20 only in the wound covered with scaffolds 
      containing HA and EGF. These results demonstrate that the scaffolds containing HA 
      and EGF can enhance wound healing.
CI  - Copyright (c) 2014 Elsevier B.V. All rights reserved.
FAU - Su, Zhongchun
AU  - Su Z
AD  - Institute of Biomedicine, National Engineering Research Center of Genetic 
      Medicine, Key Lab for Genetic Medicine of Guangdong Province, Jinan University, 
      Guangzhou 510632, China.
FAU - Ma, Huan
AU  - Ma H
AD  - Institute of Biomedicine, National Engineering Research Center of Genetic 
      Medicine, Key Lab for Genetic Medicine of Guangdong Province, Jinan University, 
      Guangzhou 510632, China.
FAU - Wu, Zhengzheng
AU  - Wu Z
AD  - Institute of Biomedicine, National Engineering Research Center of Genetic 
      Medicine, Key Lab for Genetic Medicine of Guangdong Province, Jinan University, 
      Guangzhou 510632, China.
FAU - Zeng, Huilan
AU  - Zeng H
AD  - Department of Hematology, The First Affiliated Hospital, Jinan University, 
      Guangzhou 510632, China.
FAU - Li, Zhizhong
AU  - Li Z
AD  - Department of Bone, The First Affiliated Hospital, Jinan University, Guangzhou 
      510632, China.
FAU - Wang, Yuechun
AU  - Wang Y
AD  - Department of Physiology, School of Medicine, Jinan University, Guangzhou 510632, 
      China.
FAU - Liu, Gexiu
AU  - Liu G
AD  - Department of Physiology, School of Medicine, Jinan University, Guangzhou 510632, 
      China.
FAU - Xu, Bin
AU  - Xu B
AD  - Grandhope Biotech Co., Ltd., Building D, #408, Guangzhou International Business 
      Incubator, Guangzhou Science Park, Guangzhou 510663, Guangdong, China.
FAU - Lin, Yongliang
AU  - Lin Y
AD  - Grandhope Biotech Co., Ltd., Building D, #408, Guangzhou International Business 
      Incubator, Guangzhou Science Park, Guangzhou 510663, Guangdong, China.
FAU - Zhang, Peng
AU  - Zhang P
AD  - Grandhope Biotech Co., Ltd., Building D, #408, Guangzhou International Business 
      Incubator, Guangzhou Science Park, Guangzhou 510663, Guangdong, China.
FAU - Wei, Xing
AU  - Wei X
AD  - Institute of Biomedicine, National Engineering Research Center of Genetic 
      Medicine, Key Lab for Genetic Medicine of Guangdong Province, Jinan University, 
      Guangzhou 510632, China. Electronic address: wei70@hotmail.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140719
PL  - Netherlands
TA  - Mater Sci Eng C Mater Biol Appl
JT  - Materials science & engineering. C, Materials for biological applications
JID - 101484109
RN  - 62229-50-9 (Epidermal Growth Factor)
RN  - 9004-61-9 (Hyaluronic Acid)
SB  - IM
MH  - Animals
MH  - Cell Survival/drug effects
MH  - Epidermal Growth Factor/chemistry/*pharmacology
MH  - Hyaluronic Acid/chemistry/*pharmacology
MH  - Mice
MH  - Microscopy, Electron, Scanning
MH  - NIH 3T3 Cells
MH  - Skin/*drug effects/injuries
MH  - Skin Transplantation
MH  - Swine
MH  - Tissue Scaffolds/*chemistry
MH  - Wound Healing/*drug effects
OTO - NOTNLM
OT  - Decellularized scaffolds
OT  - Growth factors
OT  - Skin repair
EDAT- 2014/10/05 06:00
MHDA- 2015/06/17 06:00
CRDT- 2014/10/05 06:00
PHST- 2014/02/17 00:00 [received]
PHST- 2014/05/31 00:00 [revised]
PHST- 2014/07/13 00:00 [accepted]
PHST- 2014/10/05 06:00 [entrez]
PHST- 2014/10/05 06:00 [pubmed]
PHST- 2015/06/17 06:00 [medline]
AID - S0928-4931(14)00446-9 [pii]
AID - 10.1016/j.msec.2014.07.039 [doi]
PST - ppublish
SO  - Mater Sci Eng C Mater Biol Appl. 2014 Nov;44:440-8. doi: 
      10.1016/j.msec.2014.07.039. Epub 2014 Jul 19.