PMID- 25280731
OWN - NLM
STAT- MEDLINE
DCOM- 20151130
LR  - 20181202
IS  - 1573-6776 (Electronic)
IS  - 0141-5492 (Linking)
VI  - 37
IP  - 2
DP  - 2015 Feb
TI  - Comparison of chloromethyl-dialkylcarbocyanine and green fluorescent protein for 
      labeling human umbilical mesenchymal stem cells.
PG  - 437-47
LID - 10.1007/s10529-014-1692-1 [doi]
AB  - To investigate the effectiveness of chloromethyl-dialkylcarbocyanine (CM-Dil) and 
      green florescent protein (GFP) for tracking transplanted stem human umbilical 
      cord-derived mesenchymal stem cells (hUC-MSCs), hUC-MSCs were labeled with CM-Dil 
      or GFP and transplanted into guinea pigs with nasal mucosa radiation injury. In 
      vitro and in vivo labeling efficiency was investigated by flow cytometry and 
      fluorescence microscopy. Proliferation and multi-lineage differentiation 
      potential of labeled hUC-MSCs were assessed via cell quantification and specific 
      staining. In vitro CM-Dil and GFP labeling efficiency was 95 +/- 12.2 and 90 +/- 8 % 
      at first passage, respectively. Labeled hUC-MSCs were detected by fluorescence 
      microscopy 10 days (CM-Dil) or 20 days (GFP) after transplantation. Neither type 
      of cell labeling affected the multi-differentiation potential but GFP labeling 
      inhibited hUC-MSC proliferation due to virus toxicity. hUC-MSCs can be labeled 
      with either CM-Dil or GFP with high efficiency without impacting 
      multi-differentiation potential.
FAU - Ji, Fang
AU  - Ji F
AD  - Department of Neurology, First Affiliated Hospital of College of Medicine, 
      Zhejiang University, Hangzhou, China, jifang0712@163.com.
FAU - Duan, Hong-Gang
AU  - Duan HG
FAU - Zheng, Chun-Quan
AU  - Zheng CQ
FAU - Li, Jing
AU  - Li J
LA  - eng
PT  - Journal Article
DEP - 20141004
PL  - Netherlands
TA  - Biotechnol Lett
JT  - Biotechnology letters
JID - 8008051
RN  - 0 (Carbocyanines)
RN  - 147336-22-9 (Green Fluorescent Proteins)
SB  - IM
MH  - Animals
MH  - Carbocyanines/*chemistry/metabolism
MH  - Cell Differentiation
MH  - Cell Tracking/*methods
MH  - Green Fluorescent Proteins/*chemistry/genetics/metabolism
MH  - Guinea Pigs
MH  - Humans
MH  - Male
MH  - Mesenchymal Stem Cell Transplantation
MH  - Mesenchymal Stem Cells/chemistry/*cytology/metabolism
MH  - Phenotype
MH  - Staining and Labeling
MH  - Umbilical Cord/cytology
EDAT- 2014/10/05 06:00
MHDA- 2015/12/15 06:00
CRDT- 2014/10/05 06:00
PHST- 2014/07/28 00:00 [received]
PHST- 2014/09/25 00:00 [accepted]
PHST- 2014/10/05 06:00 [entrez]
PHST- 2014/10/05 06:00 [pubmed]
PHST- 2015/12/15 06:00 [medline]
AID - 10.1007/s10529-014-1692-1 [doi]
PST - ppublish
SO  - Biotechnol Lett. 2015 Feb;37(2):437-47. doi: 10.1007/s10529-014-1692-1. Epub 2014 
      Oct 4.