PMID- 25280773
OWN - NLM
STAT- MEDLINE
DCOM- 20170207
LR  - 20201209
IS  - 1879-3169 (Electronic)
IS  - 0378-4274 (Linking)
VI  - 232
IP  - 1
DP  - 2015 Jan 5
TI  - Hyaluronan regulates chemical allergen-induced IL-18 production in human 
      keratinocytes.
PG  - 89-97
LID - S0378-4274(14)01360-5 [pii]
LID - 10.1016/j.toxlet.2014.09.026 [doi]
AB  - Interleukin-18 (IL-18) has been shown to play a key proximal role in the 
      induction of allergic contact dermatitis. Low molecular weight hyaluronan 
      (LMWHA), an endogenous molecule and a member of the so-called damage associated 
      molecular patterns (DAMPs), has been suggested to elicit immune-stimulatory 
      effects. The purpose of this study was to examine the role of hyaluronan (HA) 
      degradation in IL-18 production in human keratinocytes following stimulation with 
      the contact sensitizers 2,4-dinitrochlorobenzene (DNCB) and PPD. IL-18 production 
      in the human keratinocyte cell line NCTC2544 was measured by ELISA, whereas 
      changes in HA metabolism were determined by Real-time PCR and immunofluorescence. 
      Both contact allergens were able to enhance hyaluronidase (HYAL) 1 and 2 
      expression inducing HA degradation. Modulation of HA production, by HYAL or 
      aristolochic acid pre-treatment, resulted in a significant reduction of contact 
      allergen-induced IL-18 production. Oxidative stress appears to be the initial 
      step in KC activation, as all the sequels of events can be blocked using 
      antioxidants. This is the first indication that LMWHA can act as a DAMP in 
      keratinocytes. In conclusion LMWHA fragments are important mediators in the 
      process of contact sensitisation leading to IL-18 dependent responses.
CI  - Copyright (c) 2014 Elsevier Ireland Ltd. All rights reserved.
FAU - Nikitovic, Dragana
AU  - Nikitovic D
AD  - Laboratory of Anatomy-Histology-Embryology, School of Medicine, University of 
      Crete, Heraklion, Greece. Electronic address: dnikitovic@med.uoc.gr.
FAU - Berdiaki, Aikaterini
AU  - Berdiaki A
AD  - Laboratory of Anatomy-Histology-Embryology, School of Medicine, University of 
      Crete, Heraklion, Greece.
FAU - Galbiati, Valentina
AU  - Galbiati V
AD  - Laboratory of Toxicology, DiSFeB, Universita degli Studi di Milano, Italy.
FAU - Kavasi, Rafaela-Maria
AU  - Kavasi RM
AD  - Laboratory of Anatomy-Histology-Embryology, School of Medicine, University of 
      Crete, Heraklion, Greece.
FAU - Papale, Angela
AU  - Papale A
AD  - Laboratory of Toxicology, DiSFeB, Universita degli Studi di Milano, Italy.
FAU - Tsatsakis, Aristidis
AU  - Tsatsakis A
AD  - Department of Forensic Sciences and Toxicology, University of Crete, Heraklion, 
      Greece.
FAU - Tzanakakis, George N
AU  - Tzanakakis GN
AD  - Laboratory of Anatomy-Histology-Embryology, School of Medicine, University of 
      Crete, Heraklion, Greece.
FAU - Corsini, Emanuela
AU  - Corsini E
AD  - Laboratory of Toxicology, DiSFeB, Universita degli Studi di Milano, Italy.
LA  - eng
PT  - Journal Article
DEP - 20141001
PL  - Netherlands
TA  - Toxicol Lett
JT  - Toxicology letters
JID - 7709027
RN  - 0 (Antioxidants)
RN  - 0 (Aristolochic Acids)
RN  - 0 (CD44 protein, human)
RN  - 0 (Cell Adhesion Molecules)
RN  - 0 (Dinitrochlorobenzene)
RN  - 0 (GPI-Linked Proteins)
RN  - 0 (Hyaluronan Receptors)
RN  - 0 (Interleukin-18)
RN  - 0 (Phenylenediamines)
RN  - 0 (Reactive Oxygen Species)
RN  - 9004-61-9 (Hyaluronic Acid)
RN  - 94218WFP5T (aristolochic acid I)
RN  - EC 3.2.1.25 (Hyal2 protein, human)
RN  - EC 3.2.1.35 (Hyaluronoglucosaminidase)
RN  - U770QIT64J (4-phenylenediamine)
SB  - IM
MH  - Antioxidants/pharmacology
MH  - Aristolochic Acids/pharmacology
MH  - Cell Adhesion Molecules/metabolism
MH  - Cell Line
MH  - Dermatitis, Allergic Contact/*etiology/immunology/metabolism
MH  - Dinitrochlorobenzene/*toxicity
MH  - GPI-Linked Proteins/metabolism
MH  - Humans
MH  - Hyaluronan Receptors/metabolism
MH  - Hyaluronic Acid/immunology/*metabolism
MH  - Hyaluronoglucosaminidase/metabolism
MH  - Interleukin-18/immunology/*metabolism
MH  - Keratinocytes/*drug effects/immunology/metabolism
MH  - Oxidative Stress/drug effects
MH  - Phenylenediamines/*toxicity
MH  - Reactive Oxygen Species/metabolism
MH  - Signal Transduction/drug effects
MH  - Up-Regulation
OTO - NOTNLM
OT  - Hyaluronan
OT  - Interleukin 18
OT  - Keratinocytes
OT  - Low molecular weight hyaluronan
OT  - Reactive oxygen species
EDAT- 2014/10/05 06:00
MHDA- 2017/02/09 06:00
CRDT- 2014/10/05 06:00
PHST- 2014/07/31 00:00 [received]
PHST- 2014/09/26 00:00 [revised]
PHST- 2014/09/29 00:00 [accepted]
PHST- 2014/10/05 06:00 [entrez]
PHST- 2014/10/05 06:00 [pubmed]
PHST- 2017/02/09 06:00 [medline]
AID - S0378-4274(14)01360-5 [pii]
AID - 10.1016/j.toxlet.2014.09.026 [doi]
PST - ppublish
SO  - Toxicol Lett. 2015 Jan 5;232(1):89-97. doi: 10.1016/j.toxlet.2014.09.026. Epub 
      2014 Oct 1.