PMID- 25284549
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20150212
LR  - 20220317
IS  - 2247-2452 (Print)
IS  - 2247-2452 (Linking)
VI  - 13
IP  - 3
DP  - 2014 Sep
TI  - Analysis of carious dentine using FTIR and ToF-SIMS.
PG  - 735-44
AB  - Apart from the Maillard reaction, other processes, such as esterification, take 
      place in carious tissue. The aim of the present study was to analyse sound and 
      carious dentine in terms of ester groups and their reaction with hydrazine 
      derivate using Fourier Transform Infrared Spectroscopy (FTIR) and Time-of-Flight 
      Secondary Ion Mass Spectrometry (ToF-SIMS). Carious and sound dentine from human 
      premolars were excavated in three series (Experimental Parts I-III) and separated 
      into inner and outer layers of carious dentine. The excavated tooth material was 
      analysed with FTIR (Part I). Carious and sound dentine were also exposed to 
      different chemical treatments and analysed with FTIR-Attenuated Total Reflectance 
      (FTIR-ATR; Part II) and ToF-SIMS (Part III). The FTIR absorption spectra showed 
      that the carious tissue contained ester groups, not detected in sound dentine. 
      The results also indicated a higher occurrence of ester groups in the inner 
      dental caries layer than in the outer ones. Potential binding to these ester 
      groups by hydrazine derivative was observed after different chemical treatments 
      with both FTIR-ATR and ToF-SIMS. The results of the present study revealed ester 
      groups unique to the carious dentine which, after reaction with hydrazine 
      derivative, form a covalent bond not found in sound dentine. The staining of 
      carious unique groups would be clinically helpful in detection and prevention 
      unnecessary removal of sound dentine.
FAU - Almhojd, Ulrica S
AU  - Almhojd US
AD  - Department of Cariology, Institute of Odontology, The Sahlgrenska Academy, 
      University of Gothenburg, Gothenburg, Sweden; Tel: +46-31-7863266; e-mail: 
      ulrica.almhojd@odontologi.gu.se.
FAU - Noren, Jorgen G
AU  - Noren JG
FAU - Arvidsson, Anna
AU  - Arvidsson A
FAU - Nilsson, Ake
AU  - Nilsson A
FAU - Lingstrom, Peter
AU  - Lingstrom P
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Oral Health Dent Manag
JT  - Oral health and dental management
JID - 101568098
EDAT- 2014/10/07 06:00
MHDA- 2014/10/07 06:01
CRDT- 2014/10/07 06:00
PHST- 2014/10/07 06:00 [entrez]
PHST- 2014/10/07 06:00 [pubmed]
PHST- 2014/10/07 06:01 [medline]
PST - ppublish
SO  - Oral Health Dent Manag. 2014 Sep;13(3):735-44.