PMID- 25284972
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20211021
IS  - 1081-3829 (Print)
IS  - 1081-3829 (Linking)
VI  - 18
IP  - 8
DP  - 2012
TI  - Interleukin-17 Induces Expression of Chemokines and Cytokines in Prostatic 
      Epithelial Cells but Does Not Stimulate Cell Growth In Vitro.
PG  - 629-644
AB  - BACKGROUND: Interleukin-17 (IL-17A) expression is increased in prostate cancer. 
      This study investigated the expression of IL-17A receptor C (IL-17RC) in 
      prostatic intraepithelial neoplasia (PIN) lesions and the effects of IL-17A on 
      prostatic epithelial cells in in-vitro studies. METHODS: IL-17RC expression in 
      human and rodent prostate tissues was detected by immunohistochemistry. 
      Quantitative real-time reverse-transcription polymerase chain reaction (qRT-PCR) 
      and Western blot analyses were used to determine mRNA and protein expression in 
      human and mouse prostatic epithelial cell lines. RESULTS: IL-17RC protein was 
      increased in human and rodent PIN lesions, compared to the normal human and 
      rodent prostatic epithelium. IL-17A treatment activated the Nuclear Factor-kappaB 
      (NF-kappaB) and/or Extracellular signal-Regulated Kinase (ERK) pathways in human PIN 
      and LNCaP cell lines as well as mouse prostate cancer cell line TRAMP-C1. IL-17A 
      treatment did not affect cell growth of the cell lines studied. However, IL-17A 
      induced expression of CXCL1, CXCL2, CCL2, CCL5, and IL-6 in human and mouse 
      prostatic epithelial cell lines. When the full-length IL-17RC was over-expressed 
      in human PIN and LNCaP cell lines, activation of NF-kappaB and/or ERK pathways and 
      expression of CXCL1, CXCL2, and CCL5 chemokines were significantly enhanced upon 
      IL-17A treatment. CONCLUSION: These findings suggest that the prostatic 
      epithelial cells in PIN lesions may respond to IL-17A stimuli with augmented 
      synthesis of chemokines, due to increased IL-17RC expression.
FAU - You, Zongbing
AU  - You Z
AD  - Department of Structural & Cellular Biology, Department of Orthopaedic Surgery, 
      Tulane Cancer Center, Louisiana Cancer Research Consortium, Tulane Center for 
      Stem Cell Research and Regenerative Medicine, Tulane Center for Aging, Tulane 
      University School of Medicine, New Orleans, Louisiana 70112.
FAU - Ge, Dongxia
AU  - Ge D
AD  - Department of Structural & Cellular Biology, Department of Orthopaedic Surgery, 
      Tulane Cancer Center, Louisiana Cancer Research Consortium, Tulane Center for 
      Stem Cell Research and Regenerative Medicine, Tulane Center for Aging, Tulane 
      University School of Medicine, New Orleans, Louisiana 70112.
FAU - Liu, Sen
AU  - Liu S
AD  - Department of Structural & Cellular Biology, Department of Orthopaedic Surgery, 
      Tulane Cancer Center, Louisiana Cancer Research Consortium, Tulane Center for 
      Stem Cell Research and Regenerative Medicine, Tulane Center for Aging, Tulane 
      University School of Medicine, New Orleans, Louisiana 70112.
FAU - Zhang, Qiuyang
AU  - Zhang Q
AD  - Department of Structural & Cellular Biology, Department of Orthopaedic Surgery, 
      Tulane Cancer Center, Louisiana Cancer Research Consortium, Tulane Center for 
      Stem Cell Research and Regenerative Medicine, Tulane Center for Aging, Tulane 
      University School of Medicine, New Orleans, Louisiana 70112.
FAU - Borowsky, Alexander D
AU  - Borowsky AD
AD  - Department of Pathology & Laboratory Medicine and Center for Comparative 
      Medicine, University of California Davis, Davis, California 95616.
FAU - Melamed, Jonathan
AU  - Melamed J
AD  - Department of Pathology, New York University School of Medicine, New York, New 
      York 10016.
LA  - eng
GR  - P20 GM103518/GM/NIGMS NIH HHS/United States
GR  - P20 RR020152/RR/NCRR NIH HHS/United States
GR  - P30 CA093373/CA/NCI NIH HHS/United States
PT  - Journal Article
PL  - United States
TA  - Int J Med Biol Front
JT  - International journal of medical and biological frontiers
JID - 9704965
PMC - PMC4180499
MID - NIHMS464287
OTO - NOTNLM
OT  - IL-17
OT  - PIN
OT  - prostate cancer
COIS- Conflicts of Interest The authors have no conflicts of interest in publication of 
      this chapter.
EDAT- 2012/01/01 00:00
MHDA- 2012/01/01 00:01
PMCR- 2014/10/01
CRDT- 2014/10/07 06:00
PHST- 2014/10/07 06:00 [entrez]
PHST- 2012/01/01 00:00 [pubmed]
PHST- 2012/01/01 00:01 [medline]
PHST- 2014/10/01 00:00 [pmc-release]
PST - ppublish
SO  - Int J Med Biol Front. 2012;18(8):629-644.