PMID- 25285506
OWN - NLM
STAT- MEDLINE
DCOM- 20150611
LR  - 20230307
IS  - 1944-9097 (Electronic)
IS  - 0362-028X (Linking)
VI  - 77
IP  - 10
DP  - 2014 Oct
TI  - Lateral-flow assay for rapid quantitative detection of clorprenaline residue in 
      swine urine.
PG  - 1824-9
LID - 10.4315/0362-028X.JFP-14-103 [doi]
AB  - Clorprenaline (CLP), a beta2-adrenergic agonist, was first found in veterinary drugs 
      for cold treatment in China in 2013. It is a potential new lean meat-boosting 
      feed additive because it can promote animal muscular mass growth and decrease fat 
      accumulation. A competitive colloidal gold-based lateral flow immunoassay system 
      with a portable strip reader was successfully developed for rapid quantitative 
      detection of CLP residue in swine urine. The detection system was optimized so 
      that the detection can be completed within 9 min with a limit of detection of 
      0.15 mug . liter(-1). The assay exhibited good linear range from 3.0 to 20.0 mug . 
      liter(-1), with reliable correlation of 0.9970 and with no obvious cross-reaction 
      with five other beta2-agonist compounds. Twenty spiked swine urine samples were 
      tested by lateral flow immunoassay and liquid chromatography-tandem mass 
      spectrometry to confirm the accuracy of the system. Results show good correlation 
      between the two methods. This method is rapid, sensitive, specific, and 
      convenient. It can be applied in the field for on-site detection of CLP in urine 
      samples.
FAU - Peng, Tao
AU  - Peng T
AD  - State Key Laboratory of Food Science and Technology, Nanchang University, 235 
      Nanjing East Road, Nanchang 330047, People's Republic of China.
FAU - Zhang, Fu S
AU  - Zhang FS
AD  - Jiangxi Agricultural Product Quality Safety and Inspection Center, 151 Wenjiao 
      Road, Nanchang 330077, People's Republic of China.
FAU - Yang, Wan C
AU  - Yang WC
AD  - Jiangxi Zodolabs Bioengineering Co., Ltd., 235 Nanjing East Road, Nanchang 
      330047, People's Republic of China.
FAU - Li, Dong X
AU  - Li DX
AD  - Jiangxi Agricultural Product Quality Safety and Inspection Center, 151 Wenjiao 
      Road, Nanchang 330077, People's Republic of China.
FAU - Chen, Yuan
AU  - Chen Y
AD  - Jiangxi Zodolabs Bioengineering Co., Ltd., 235 Nanjing East Road, Nanchang 
      330047, People's Republic of China.
FAU - Xiong, Yong H
AU  - Xiong YH
AD  - State Key Laboratory of Food Science and Technology, Nanchang University, 235 
      Nanjing East Road, Nanchang 330047, People's Republic of China.
FAU - Wei, Hua
AU  - Wei H
AD  - State Key Laboratory of Food Science and Technology, Nanchang University, 235 
      Nanjing East Road, Nanchang 330047, People's Republic of China.
FAU - Lai, Wei H
AU  - Lai WH
AD  - State Key Laboratory of Food Science and Technology, Nanchang University, 235 
      Nanjing East Road, Nanchang 330047, People's Republic of China. 
      talktolaiwh@163.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Food Prot
JT  - Journal of food protection
JID - 7703944
RN  - 0 (Gold Colloid)
RN  - 3W4327W76O (clorprenaline)
RN  - L628TT009W (Isoproterenol)
SB  - IM
MH  - Animals
MH  - Calibration
MH  - China
MH  - Chromatography, Affinity
MH  - Chromatography, Liquid
MH  - Cross Reactions
MH  - Drug Residues/*analysis
MH  - Gold Colloid/chemistry
MH  - Isoproterenol/*analogs & derivatives/urine
MH  - Meat
MH  - Reproducibility of Results
MH  - Swine
MH  - Tandem Mass Spectrometry
MH  - Urinalysis/*methods/*veterinary
EDAT- 2014/10/07 06:00
MHDA- 2015/06/13 06:00
CRDT- 2014/10/07 06:00
PHST- 2014/10/07 06:00 [entrez]
PHST- 2014/10/07 06:00 [pubmed]
PHST- 2015/06/13 06:00 [medline]
AID - S0362-028X(23)05083-4 [pii]
AID - 10.4315/0362-028X.JFP-14-103 [doi]
PST - ppublish
SO  - J Food Prot. 2014 Oct;77(10):1824-9. doi: 10.4315/0362-028X.JFP-14-103.