PMID- 25286178
OWN - NLM
STAT- MEDLINE
DCOM- 20180207
LR  - 20201209
IS  - 1879-0984 (Electronic)
IS  - 0166-0934 (Linking)
VI  - 210
DP  - 2014 Dec 15
TI  - A simple centrifugation method for improving the detection of Ostreid 
      herpesvirus-1 (OsHV-1) in natural seawater samples with an assessment of the 
      potential for particulate attachment.
PG  - 59-66
LID - S0166-0934(14)00378-4 [pii]
LID - 10.1016/j.jviromet.2014.09.023 [doi]
AB  - Ostreid herpesvirus-1 (OsHV-1) is responsible for massive mortality events in 
      commercially farmed Pacific oysters (Crassostrea gigas) in Australia, New 
      Zealand, Europe and the USA. Economic losses have been severe in many countries 
      since 2008, associated with a strain known as OsHV-1mu-var. Despite intensive 
      studies of the virus itself, there is almost no information on its detection in 
      natural seawater, how it is spread over wide geographic distance in water or on 
      how it is transmitted from oyster to oyster via seawater. The aim of the current 
      work was to (1) assess and compare several centrifugation methods in order to 
      detect OsHV-1 in natural seawater samples using real-time quantitative PCR, in 
      such a way that large numbers of samples could be processed efficiently and (2) 
      assess the potential for particulate attachment of OsHV-1 using filtration. 
      Compared to testing unprocessed seawater samples, centrifugation of seawater at 
      1000xg for 20 min with testing of the pellet improved OsHV-1 detection rates by 
      two fold. Results suggest that OsHV-1 may be attached to particles large enough 
      to be pelleted at low g-force, as well as in the form of small particles, free 
      virus or free viral DNA. Filtration of seawater using low protein binding filters 
      could not be used to assess OsHV-1 particle attachment, due to interactions 
      between particles, free virus or free viral DNA and the membranes.
CI  - Copyright (c) 2014 Elsevier B.V. All rights reserved.
FAU - Evans, O
AU  - Evans O
AD  - University of Sydney, Faculty of Veterinary Science, 425 Werombi Road, Camden, 
      New South Wales 2570, Australia.
FAU - Paul-Pont, I
AU  - Paul-Pont I
AD  - University of Sydney, Faculty of Veterinary Science, 425 Werombi Road, Camden, 
      New South Wales 2570, Australia.
FAU - Hick, P
AU  - Hick P
AD  - University of Sydney, Faculty of Veterinary Science, 425 Werombi Road, Camden, 
      New South Wales 2570, Australia.
FAU - Whittington, R J
AU  - Whittington RJ
AD  - University of Sydney, Faculty of Veterinary Science, 425 Werombi Road, Camden, 
      New South Wales 2570, Australia. Electronic address: 
      richard.whittington@sydney.edu.au.
LA  - eng
PT  - Comparative Study
PT  - Journal Article
DEP - 20141005
PL  - Netherlands
TA  - J Virol Methods
JT  - Journal of virological methods
JID - 8005839
RN  - 0 (DNA, Viral)
RN  - Ostreid herpesvirus 1
SB  - IM
MH  - Animals
MH  - Centrifugation/methods/*veterinary
MH  - Crassostrea/*virology
MH  - DNA Viruses/genetics/*isolation & purification
MH  - DNA, Viral/genetics
MH  - Real-Time Polymerase Chain Reaction/veterinary
MH  - Seawater/virology
OTO - NOTNLM
OT  - Centrifugation
OT  - Detection
OT  - Filtration
OT  - OsHV-1
OT  - Oyster
OT  - Seawater
EDAT- 2014/10/07 06:00
MHDA- 2014/10/07 06:01
CRDT- 2014/10/07 06:00
PHST- 2014/08/25 00:00 [received]
PHST- 2014/09/20 00:00 [revised]
PHST- 2014/09/24 00:00 [accepted]
PHST- 2014/10/07 06:00 [entrez]
PHST- 2014/10/07 06:00 [pubmed]
PHST- 2014/10/07 06:01 [medline]
AID - S0166-0934(14)00378-4 [pii]
AID - 10.1016/j.jviromet.2014.09.023 [doi]
PST - ppublish
SO  - J Virol Methods. 2014 Dec 15;210:59-66. doi: 10.1016/j.jviromet.2014.09.023. Epub 
      2014 Oct 5.