PMID- 25286938
OWN - NLM
STAT- MEDLINE
DCOM- 20150615
LR  - 20211021
IS  - 2053-230X (Electronic)
IS  - 2053-230X (Linking)
VI  - 70
IP  - Pt 10
DP  - 2014 Oct
TI  - Purification, crystallization and preliminary X-ray crystallographic analysis of 
      human IL-18 and its extracellular complexes.
PG  - 1351-6
LID - 10.1107/S2053230X14016926 [doi]
AB  - Interleukin-18 (IL-18), a pro-inflammatory cytokine belonging to the 
      interleukin-1 (IL-1) family, is involved in the pathogenesis of 
      autoimmune/autoinflammatory and allergic diseases such as juvenile idiopathic 
      arthritis and bronchial asthma. IL-18 forms a signalling complex with the IL-18 
      receptor alpha (IL-18Ralpha) and beta (IL-18Rbeta) chains; however, the detailed activation 
      mechanism remains unclear. Here, the IL-18-IL-18Ralpha binary and 
      IL-18-IL-18Ralpha-IL-18Rbeta ternary complexes were purified and crystallized as well as 
      IL-18 alone. An X-ray diffraction data set for IL-18 was collected to 2.33 A 
      resolution from a crystal belonging to space group P21, with unit-cell parameters 
      a = 68.15, b = 79.51, c = 73.46 A, beta = 100.97 degrees . Crystals of both the IL-18 binary 
      and ternary complexes belonging to the orthorhombic space groups P21212 and 
      P212121, respectively, diffracted to 3.10 A resolution. Unit-cell parameters were 
      determined as a = 135.49, b = 174.81, c = 183.40 A for the binary complex and a = 
      72.56, b = 111.56, c = 134.57 A for the ternary complex.
FAU - Kimura, Takeshi
AU  - Kimura T
AD  - Department of Pediatrics, Graduate School of Medicine, Gifu University, Yanagido 
      1-1, Gifu 501-1194, Japan.
FAU - Tsutsumi, Naotaka
AU  - Tsutsumi N
AD  - Department of Molecular Engineering, Graduate School of Engineering, Kyoto 
      University, Katsura, Nishikyo-ku, Kyoto 615-8510, Japan.
FAU - Arita, Kyohei
AU  - Arita K
AD  - Graduate School of Medical Life Science, Yokohama City University, 1-7-29 
      Suehiro-cho,Tsurumi-ku, Yokohama, Japan.
FAU - Ariyoshi, Mariko
AU  - Ariyoshi M
AD  - Department of Molecular Engineering, Graduate School of Engineering, Kyoto 
      University, Katsura, Nishikyo-ku, Kyoto 615-8510, Japan.
FAU - Ohnishi, Hidenori
AU  - Ohnishi H
AD  - Department of Pediatrics, Graduate School of Medicine, Gifu University, Yanagido 
      1-1, Gifu 501-1194, Japan.
FAU - Kondo, Naomi
AU  - Kondo N
AD  - Department of Pediatrics, Graduate School of Medicine, Gifu University, Yanagido 
      1-1, Gifu 501-1194, Japan.
FAU - Shirakawa, Masahiro
AU  - Shirakawa M
AD  - Department of Molecular Engineering, Graduate School of Engineering, Kyoto 
      University, Katsura, Nishikyo-ku, Kyoto 615-8510, Japan.
FAU - Kato, Zenichiro
AU  - Kato Z
AD  - Department of Pediatrics, Graduate School of Medicine, Gifu University, Yanagido 
      1-1, Gifu 501-1194, Japan.
FAU - Tochio, Hidehito
AU  - Tochio H
AD  - Department of Molecular Engineering, Graduate School of Engineering, Kyoto 
      University, Katsura, Nishikyo-ku, Kyoto 615-8510, Japan.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140925
PL  - United States
TA  - Acta Crystallogr F Struct Biol Commun
JT  - Acta crystallographica. Section F, Structural biology communications
JID - 101620319
RN  - 0 (IL18 protein, human)
RN  - 0 (Interleukin-18)
RN  - 0 (Receptors, Interleukin-18)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Chromatography, Gel
MH  - Crystallization
MH  - Crystallography, X-Ray
MH  - Humans
MH  - Interleukin-18/*chemistry/isolation & purification
MH  - Molecular Sequence Data
MH  - Protein Binding
MH  - Receptors, Interleukin-18/*chemistry/isolation & purification
MH  - Sf9 Cells
MH  - Spodoptera
PMC - PMC4188078
OTO - NOTNLM
OT  - IL-1 superfamily
OT  - allergy
OT  - autoimmunity
OT  - immunity
OT  - inflammation
OT  - interleukin-18
OT  - interleukin-18 receptor alpha
OT  - interleukin-18 receptor beta
EDAT- 2014/10/08 06:00
MHDA- 2015/06/16 06:00
PMCR- 2016/10/01
CRDT- 2014/10/08 06:00
PHST- 2014/06/10 00:00 [received]
PHST- 2014/07/22 00:00 [accepted]
PHST- 2014/10/08 06:00 [entrez]
PHST- 2014/10/08 06:00 [pubmed]
PHST- 2015/06/16 06:00 [medline]
PHST- 2016/10/01 00:00 [pmc-release]
AID - S2053230X14016926 [pii]
AID - rl5077 [pii]
AID - 10.1107/S2053230X14016926 [doi]
PST - ppublish
SO  - Acta Crystallogr F Struct Biol Commun. 2014 Oct;70(Pt 10):1351-6. doi: 
      10.1107/S2053230X14016926. Epub 2014 Sep 25.