PMID- 25289028
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20211021
IS  - 1792-0981 (Print)
IS  - 1792-1015 (Electronic)
IS  - 1792-0981 (Linking)
VI  - 8
IP  - 5
DP  - 2014 Nov
TI  - Roles of the co-culture of human umbilical cord Wharton's jelly-derived 
      mesenchymal stem cells with rat pancreatic cells in the treatment of rats with 
      diabetes mellitus.
PG  - 1389-1396
AB  - The aim of the present study was to investigate the roles of the co-culture of 
      human umbilical cord Wharton's jelly-derived mesenchymal stem cells (hUC-MSCs) 
      with rat pancreatic cells in the treatment of rats with diabetes mellitus. 
      hUC-MSCs were isolated and passaged, followed by Transwell co-culture with rat 
      pancreatic cells. The induced islet-like cell clusters were transplanted into the 
      renal capsule in rats with streptozotocin (STZ)-induced diabetes mellitus. The 
      effects of co-culture on blood glucose levels in rats were observed. The isolated 
      hUC-MSCs expressed the specific surface markers, including cluster of 
      differentiation 44 (CD44) (91.4%), CD29 (91.3%) and CD105 (99.2%). Following 
      co-culture with hUC-MSCs for 7 and 10 days, the rat pancreatic cells were 
      strongly stained by pancreatic and duodenal homeobox-1 and human insulin. The 
      insulin and C-peptide concentrations were increased significantly compared to the 
      pure culture group. One week following the transplantation of induced islet-like 
      cells into the renal capsule, the blood glucose level of rats in the STZ 
      experimental group was significantly lower than that of the STZ control group. 
      There were notable 5-bromo-2'-deoxyuridine-positive nuclei and insulin-positive 
      cytoplasm in the renal capsule following cell transplantation. Therefore, 
      co-culture of hUC-MSCs with rat pancreatic cells can lower the blood glucose 
      levels in rats with diabetes mellitus.
FAU - Wang, Guangyu
AU  - Wang G
AD  - Ulcerous Vascular Surgical Department, Beijing TCM Hospital Affiliated to Capital 
      Medical University, Beijing 100010, P.R. China.
FAU - Li, Yong
AU  - Li Y
AD  - Ulcerous Vascular Surgical Department, Beijing TCM Hospital Affiliated to Capital 
      Medical University, Beijing 100010, P.R. China.
FAU - Wang, Yu
AU  - Wang Y
AD  - Ulcerous Vascular Surgical Department, Beijing TCM Hospital Affiliated to Capital 
      Medical University, Beijing 100010, P.R. China.
FAU - Dong, Yu
AU  - Dong Y
AD  - Ulcerous Vascular Surgical Department, Beijing TCM Hospital Affiliated to Capital 
      Medical University, Beijing 100010, P.R. China.
FAU - Wang, Fu-Sheng
AU  - Wang FS
AD  - Ulcerous Vascular Surgical Department, Beijing TCM Hospital Affiliated to Capital 
      Medical University, Beijing 100010, P.R. China.
FAU - Ding, Yi
AU  - Ding Y
AD  - Ulcerous Vascular Surgical Department, Beijing TCM Hospital Affiliated to Capital 
      Medical University, Beijing 100010, P.R. China.
FAU - Kang, Yudong
AU  - Kang Y
AD  - Ulcerous Vascular Surgical Department, Beijing TCM Hospital Affiliated to Capital 
      Medical University, Beijing 100010, P.R. China.
FAU - Xu, Xuying
AU  - Xu X
AD  - Ulcerous Vascular Surgical Department, Beijing TCM Hospital Affiliated to Capital 
      Medical University, Beijing 100010, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20140922
PL  - Greece
TA  - Exp Ther Med
JT  - Experimental and therapeutic medicine
JID - 101531947
PMC - PMC4186331
OTO - NOTNLM
OT  - Wharton's jelly
OT  - diabetes mellitus
OT  - mesenchymal stem cells
OT  - transplantation
OT  - umbilical cord
EDAT- 2014/10/08 06:00
MHDA- 2014/10/08 06:01
PMCR- 2014/09/22
CRDT- 2014/10/08 06:00
PHST- 2014/02/26 00:00 [received]
PHST- 2014/08/11 00:00 [accepted]
PHST- 2014/10/08 06:00 [entrez]
PHST- 2014/10/08 06:00 [pubmed]
PHST- 2014/10/08 06:01 [medline]
PHST- 2014/09/22 00:00 [pmc-release]
AID - etm-08-05-1389 [pii]
AID - 10.3892/etm.2014.1985 [doi]
PST - ppublish
SO  - Exp Ther Med. 2014 Nov;8(5):1389-1396. doi: 10.3892/etm.2014.1985. Epub 2014 Sep 
      22.