PMID- 25289050
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20211021
IS  - 1792-0981 (Print)
IS  - 1792-1015 (Electronic)
IS  - 1792-0981 (Linking)
VI  - 8
IP  - 5
DP  - 2014 Nov
TI  - Karyotype stability of human umbilical cord-derived mesenchymal stem cells during 
      in vitro culture.
PG  - 1508-1512
AB  - The aim of this study was to investigate whether the chromosomes of human 
      umbilical cord-derived mesenchymal stem cells (hUCMSCs) change following in vitro 
      culture for several generations. In the present study, umbilical cords from two 
      healthy infants following cesarean delivery were collected aseptically and 
      hUCMSCs were isolated by digestion with collagenase and trypsin, and then 
      cultured in vitro. hUCMSCs with fibroblastic morphology were presented from the 
      human umbilical cord tissue after 7 days of adherent culture. When cultured for 6 
      passages in vitro, the hUCMSCs maintained a stable spindle-shaped morphology. 
      Cells reached the logarithmic growth phase after 3-4 days of culture. In 
      addition, CD13, CD29, CD44, CD90 and CD105 were highly expressed in generations 
      P3-P6. The expression of CD31, CD34, CD45 and HLA-DR was negative. Furthermore, 
      karyotype analysis revealed a normal diploid karyotype with 46 chromosomes and no 
      abnormal changes were found in chromosome structure. These findings suggest that 
      when cultured for 6 passages in vitro, hUCMSCs maintain a stable immunophenotype 
      and chromosome structure, which provides an experimental basis for the safety of 
      hUCMSC cytotherapy.
FAU - Ruan, Zhong-Bao
AU  - Ruan ZB
AD  - Department of Cardiology, Taizhou People's Hospital, Taizhou, Jiangsu 225300, 
      P.R. China.
FAU - Zhu, Li
AU  - Zhu L
AD  - Department of Cardiology, Taizhou People's Hospital, Taizhou, Jiangsu 225300, 
      P.R. China.
FAU - Yin, Yi-Gang
AU  - Yin YG
AD  - Department of Cardiology, Taizhou People's Hospital, Taizhou, Jiangsu 225300, 
      P.R. China.
FAU - Chen, Ge-Cai
AU  - Chen GC
AD  - Department of Cardiology, Taizhou People's Hospital, Taizhou, Jiangsu 225300, 
      P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20140918
PL  - Greece
TA  - Exp Ther Med
JT  - Experimental and therapeutic medicine
JID - 101531947
PMC - PMC4186357
OTO - NOTNLM
OT  - human umbilical cord-derived mesenchymal stem cells
OT  - karyotyping
OT  - serial passage
EDAT- 2014/10/08 06:00
MHDA- 2014/10/08 06:01
PMCR- 2014/09/18
CRDT- 2014/10/08 06:00
PHST- 2014/02/22 00:00 [received]
PHST- 2014/07/23 00:00 [accepted]
PHST- 2014/10/08 06:00 [entrez]
PHST- 2014/10/08 06:00 [pubmed]
PHST- 2014/10/08 06:01 [medline]
PHST- 2014/09/18 00:00 [pmc-release]
AID - etm-08-05-1508 [pii]
AID - 10.3892/etm.2014.1977 [doi]
PST - ppublish
SO  - Exp Ther Med. 2014 Nov;8(5):1508-1512. doi: 10.3892/etm.2014.1977. Epub 2014 Sep 
      18.