PMID- 25289355 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20141007 LR - 20211021 IS - 2169-7574 (Print) IS - 2169-7574 (Electronic) IS - 2169-7574 (Linking) VI - 2 IP - 6 DP - 2014 Jun TI - A New Economic Method for Preparing Platelet-rich Plasma. PG - e162 LID - 10.1097/GOX.0000000000000109 [doi] LID - e162 AB - BACKGROUND: Although platelet-rich plasma (PRP) is nowadays a common method in various medical fields, including cosmetic surgery or dermatology, the expensiveness of the kit for processing is still a hurdle. METHODS: A new unique economic method for preparing PRP was reported. The method consists in a simple modification of a disposable 5-mL syringe that allows insertion into a common centrifuge and positioning of the syringe on the centrifuge so the PRP separates next to the tip of the syringe. Platelet-derived growth factor BB in PRP was measured under anticoagulant dextrose solution A (ACD-A) or heparin as anticoagulant and with or without prostaglandin E1 (PGE1) as a platelet aggregation suppressant. RESULTS: The new method successfully created PRP with high platelet-derived growth factor BB in all conditions, and the highest value was obtained by using ACD-A and PGE1. CONCLUSIONS: The new method is useful, and the use of ACD-A and PGE1 is the most recommended. FAU - Fukaya, Mototsugu AU - Fukaya M AD - Tsurumai Kouen Clinic, Nagoya, Japan. FAU - Ito, Asako AU - Ito A AD - Tsurumai Kouen Clinic, Nagoya, Japan. LA - eng PT - Journal Article DEP - 20140709 PL - United States TA - Plast Reconstr Surg Glob Open JT - Plastic and reconstructive surgery. Global open JID - 101622231 PMC - PMC4174234 EDAT- 2014/10/08 06:00 MHDA- 2014/10/08 06:01 PMCR- 2014/07/09 CRDT- 2014/10/08 06:00 PHST- 2013/12/26 00:00 [received] PHST- 2014/04/07 00:00 [accepted] PHST- 2014/10/08 06:00 [entrez] PHST- 2014/10/08 06:00 [pubmed] PHST- 2014/10/08 06:01 [medline] PHST- 2014/07/09 00:00 [pmc-release] AID - 10.1097/GOX.0000000000000109 [doi] PST - epublish SO - Plast Reconstr Surg Glob Open. 2014 Jul 9;2(6):e162. doi: 10.1097/GOX.0000000000000109. eCollection 2014 Jun.