PMID- 2529042
OWN - NLM
STAT- MEDLINE
DCOM- 19891115
LR  - 20190720
IS  - 0008-8749 (Print)
IS  - 0008-8749 (Linking)
VI  - 123
IP  - 2
DP  - 1989 Oct 15
TI  - Murine T helper cell clones secrete granulocyte-macrophage colony-stimulating 
      factor (GmCSF) by both interleukin-2-dependent and interleukin-2-independent 
      pathways.
PG  - 445-55
AB  - Granulocyte-macrophage colony-stimulating factor (GmCSF) is a lymphokine secreted 
      by class II major histocompatibility complex (MHC)-restricted T cells after 
      lectin or antigen stimulation. To investigate the relationship between 
      interleukin-2 (IL-2) and GmCSF production, we utilized long-term cultures of 
      porcine myelin basic protein (PMBP)-specific T helper cell clones that were 
      maintained with IL-2 in the absence of antigen or irradiated antigen-presenting 
      cells (APC). We have found that supernatants of these T cell clones contained 
      GmCSF activity after IL-2 stimulation. Inhibition of cell proliferation by 
      irradiation failed to stop GmCSF production. When these clones were stimulated 
      with PMBP and irradiated APC in the presence of anti-IL-2 receptor antibody, the 
      T cell supernatants still contained GmCSF activity. These results indicate that 
      (1) GmCSF production by T helper clones after IL-2 stimulation is independent of 
      cell proliferation and (2) antigen/MHC-stimulated GmCSF production by T cell 
      clones can occur by an IL-2-independent pathway.
FAU - Wong, R L
AU  - Wong RL
AD  - Department of Medicine, University of Connecticut Health Center, Farmington 
      06032.
FAU - Lingenheld, E G
AU  - Lingenheld EG
FAU - Fitzgerald, L
AU  - Fitzgerald L
FAU - Clark, R B
AU  - Clark RB
LA  - eng
GR  - AM-20621/AM/NIADDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Cell Immunol
JT  - Cellular immunology
JID - 1246405
RN  - 0 (Antigens)
RN  - 0 (Colony-Stimulating Factors)
RN  - 0 (Growth Substances)
RN  - 0 (Interleukin-2)
RN  - 0 (Receptors, Interleukin-2)
RN  - 83869-56-1 (Granulocyte-Macrophage Colony-Stimulating Factor)
SB  - IM
MH  - Animals
MH  - Antigens/immunology
MH  - Clone Cells
MH  - Colony-Stimulating Factors/*metabolism
MH  - Granulocyte-Macrophage Colony-Stimulating Factor
MH  - Growth Substances/*metabolism
MH  - Interleukin-2/*physiology
MH  - Lymphocyte Activation
MH  - Mice
MH  - Receptors, Interleukin-2/immunology
MH  - T-Lymphocytes, Helper-Inducer/*physiology
EDAT- 1989/10/15 00:00
MHDA- 1989/10/15 00:01
CRDT- 1989/10/15 00:00
PHST- 1989/10/15 00:00 [pubmed]
PHST- 1989/10/15 00:01 [medline]
PHST- 1989/10/15 00:00 [entrez]
AID - 0008-8749(89)90304-3 [pii]
AID - 10.1016/0008-8749(89)90304-3 [doi]
PST - ppublish
SO  - Cell Immunol. 1989 Oct 15;123(2):445-55. doi: 10.1016/0008-8749(89)90304-3.