PMID- 25290573
OWN - NLM
STAT- MEDLINE
DCOM- 20160921
LR  - 20151215
IS  - 2042-3306 (Electronic)
IS  - 0425-1644 (Linking)
VI  - 48
IP  - 1
DP  - 2016 Jan
TI  - A commercially available immunoglobulin E-based test for food allergy gives 
      inconsistent results in healthy ponies.
PG  - 109-13
LID - 10.1111/evj.12369 [doi]
AB  - REASONS FOR PERFORMING STUDY: Commercial immunoglobulin E (IgE)-based tests are 
      available for diagnosis of food allergies and are commonly used in equine 
      practice. However, these tests have been proven unreliable as a screening method 
      in man and other species, but not critically evaluated in equids. Therefore, a 
      commercially available IgE-based test for horses was evaluated. OBJECTIVES: To 
      evaluate the consistency of the results obtained with a commercially available 
      IgE-based test for food allergy diagnosis in ponies (Phase I) and to subject 
      ponies to a provocation trial with the presumed allergens (Phase II). STUDY 
      DESIGN: Allergen screening followed by experimental food provocation trials in 
      healthy ponies. METHODS: Blood samples of 17 healthy Shetland ponies were taken 
      at 2 different time points, sent blinded to a commercial laboratory for screening 
      of common food allergens and the results were evaluated for consistency (Phase 
      I). Ponies that were positive for food allergens were consecutively challenged 
      orally with each allergen separately for 14 days (Phase II). A washout period of 
      one week was applied in ponies with multiple positive results. Clinical 
      parameters and serum amyloid A were monitored during the provocation trial. 
      RESULTS: Only 7/17 ponies were negative on the IgE-based test at the 2 time 
      points, 3 had positive results twice but only one tested positive twice for the 
      same food allergen. No abnormalities were noted during the provocation trials. 
      CONCLUSIONS: This study demonstrated that this IgE-based test is not a reliable 
      screening tool for food allergy in healthy equids.
CI  - (c) 2015 EVJ Ltd.
FAU - Dupont, S
AU  - Dupont S
AD  - Laboratory of Animal Nutrition, Ghent University, Belgium.
FAU - De Spiegeleer, A
AU  - De Spiegeleer A
AD  - Laboratory of Animal Nutrition, Ghent University, Belgium.
FAU - Liu, D J X
AU  - Liu DJ
AD  - Laboratory of Animal Nutrition, Ghent University, Belgium.
FAU - Lefere, L
AU  - Lefere L
AD  - Department of Large Animal Internal Medicine, Ghent University, Belgium.
FAU - van Doorn, D A
AU  - van Doorn DA
AD  - Cavalor Equine Nutrition Research, Drongen, Belgium.
AD  - Department of Nutrition, Faculty of Veterinary Medicine, Utrecht, The 
      Netherlands.
FAU - Hesta, M
AU  - Hesta M
AD  - Laboratory of Animal Nutrition, Ghent University, Belgium.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150128
PL  - United States
TA  - Equine Vet J
JT  - Equine veterinary journal
JID - 0173320
RN  - 0 (Allergens)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
CIN - Equine Vet J. 2016 Jan;48(1):13-4. PMID: 26663407
MH  - Allergens/*immunology
MH  - Animal Feed
MH  - Animals
MH  - Food Hypersensitivity/diagnosis/*veterinary
MH  - Horse Diseases/*diagnosis
MH  - Horses
MH  - *Immunoassay
MH  - Immunoglobulin E/*immunology
MH  - Male
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
OTO - NOTNLM
OT  - food allergy
OT  - food provocation test
OT  - horse
OT  - immunoglobulin E
OT  - nutrition
OT  - pony
EDAT- 2014/10/08 06:00
MHDA- 2016/09/23 06:00
CRDT- 2014/10/08 06:00
PHST- 2014/02/18 00:00 [received]
PHST- 2014/09/01 00:00 [accepted]
PHST- 2014/10/08 06:00 [entrez]
PHST- 2014/10/08 06:00 [pubmed]
PHST- 2016/09/23 06:00 [medline]
AID - 10.1111/evj.12369 [doi]
PST - ppublish
SO  - Equine Vet J. 2016 Jan;48(1):109-13. doi: 10.1111/evj.12369. Epub 2015 Jan 28.