PMID- 25290620
OWN - NLM
STAT- MEDLINE
DCOM- 20150217
LR  - 20201222
IS  - 1090-2163 (Electronic)
IS  - 0008-8749 (Linking)
VI  - 292
IP  - 1-2
DP  - 2014 Nov-Dec
TI  - Pancreatic cancer derived exosomes regulate the expression of TLR4 in dendritic 
      cells via miR-203.
PG  - 65-9
LID - S0008-8749(14)00147-6 [pii]
LID - 10.1016/j.cellimm.2014.09.004 [doi]
AB  - MicroRNAs (miRNAs) are aberrant in many human tumors which can be transferred to 
      immune cells by tumor-derived exosomes. Dendritic cells (DCs) play an important 
      role in activation of immune response. However, the effect of tumor-derived 
      exosomes on toll-like receptor (TLR) in DCs remains unclear. We investigated the 
      influence of pancreatic cancer derived exosomes on TLR4, and downstream cytokines 
      via miR-203. Our results showed that miR-203 expressed in panc-1 cells and 
      exosomes, and upregulated in exosomes-treated DCs. TLR4 decreased after treatment 
      of exosomes and miR-203 mimics, while increased in exosomes-treated DCs by 
      miR-203 inhibitors. But the mRNA level of TLR4 was not significantly different 
      between DCs and exosomes-treated DCs. Tumor necrosis factor-alpha (TNF-alpha) and 
      interleukin-12 (IL-12) also decreased under treatment of exosomes and miR-203 
      mimics, both of which increased in exosomes-treated DCs by miR-203 inhibitors. 
      Collectively, pancreatic cancer derived exosomes downregulate TLR4 and downstream 
      cytokines in DCs via miR-203.
CI  - Copyright (c) 2014 Elsevier Inc. All rights reserved.
FAU - Zhou, Min
AU  - Zhou M
AD  - Department of General Surgery, The Second Affiliated Hospital, School of 
      Medicine, Zhejiang University, Hangzhou 310009, China.
FAU - Chen, Jionghuang
AU  - Chen J
AD  - Department of General Surgery, The Second Affiliated Hospital, School of 
      Medicine, Zhejiang University, Hangzhou 310009, China.
FAU - Zhou, Liangjing
AU  - Zhou L
AD  - Department of General Surgery, The Second Affiliated Hospital, School of 
      Medicine, Zhejiang University, Hangzhou 310009, China.
FAU - Chen, Wenchao
AU  - Chen W
AD  - Department of General Surgery, The Second Affiliated Hospital, School of 
      Medicine, Zhejiang University, Hangzhou 310009, China.
FAU - Ding, Guoping
AU  - Ding G
AD  - Department of General Surgery, The Second Affiliated Hospital, School of 
      Medicine, Zhejiang University, Hangzhou 310009, China.
FAU - Cao, Liping
AU  - Cao L
AD  - Department of General Surgery, The Second Affiliated Hospital, School of 
      Medicine, Zhejiang University, Hangzhou 310009, China. Electronic address: 
      zjulipingcao@163.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140928
PL  - Netherlands
TA  - Cell Immunol
JT  - Cellular immunology
JID - 1246405
RN  - 0 (MIRN203 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (TLR4 protein, human)
RN  - 0 (Toll-Like Receptor 4)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 187348-17-0 (Interleukin-12)
SB  - IM
MH  - Cell Line, Tumor
MH  - Dendritic Cells/*immunology
MH  - Exosomes/*immunology
MH  - Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - Interleukin-12/biosynthesis/immunology
MH  - MicroRNAs/genetics/*immunology
MH  - Pancreatic Neoplasms/genetics/*immunology
MH  - Toll-Like Receptor 4/genetics/*immunology
MH  - Tumor Necrosis Factor-alpha/biosynthesis/immunology
OTO - NOTNLM
OT  - Dendritic cells
OT  - Exosomes
OT  - MicroRNAs
OT  - Pancreatic cancer
OT  - Toll-like receptor
EDAT- 2014/10/08 06:00
MHDA- 2015/02/18 06:00
CRDT- 2014/10/08 06:00
PHST- 2014/07/05 00:00 [received]
PHST- 2014/09/19 00:00 [revised]
PHST- 2014/09/22 00:00 [accepted]
PHST- 2014/10/08 06:00 [entrez]
PHST- 2014/10/08 06:00 [pubmed]
PHST- 2015/02/18 06:00 [medline]
AID - S0008-8749(14)00147-6 [pii]
AID - 10.1016/j.cellimm.2014.09.004 [doi]
PST - ppublish
SO  - Cell Immunol. 2014 Nov-Dec;292(1-2):65-9. doi: 10.1016/j.cellimm.2014.09.004. 
      Epub 2014 Sep 28.