PMID- 25292445
OWN - NLM
STAT- MEDLINE
DCOM- 20150803
LR  - 20141118
IS  - 1439-0531 (Electronic)
IS  - 0936-6768 (Linking)
VI  - 49
IP  - 6
DP  - 2014 Dec
TI  - Effect of oestrus synchronization with PGF2alpha/eCG/hCG on luteal P4 synthesis in 
      early pregnant gilts.
PG  - 1034-42
LID - 10.1111/rda.12433 [doi]
AB  - Administration of hormones to synchronize oestrus is a useful tool in animal 
      breeding. However, exogenous ovarian stimulation may be detrimental to 
      reproductive function. This study was aimed to examine whether an oestrus 
      synchronization with PGF2alpha/eCG/hCG could affect luteal P4 synthesis in early 
      pregnant gilts. Corpora lutea (CLs) were collected on days 9, 12 and 16 of 
      pregnancy from gilts with natural (n = 16) and synchronized (n = 18) oestrus and 
      analysed for (i) the expre-ssion of steroidogenic acute regulatory protein 
      (StAR), cytochrome P450 family 11 subfamily A polypeptide (CYP11A1), and 
      3beta-hydroxysteroid dehydrogenase (3betaHSD); (ii) the concentration of P4 in the 
      luteal tissue and blood; and (iii) the expression of luteinizing hormone 
      receptors (LHR) and oestrogen receptors (ERalpha and ERbeta). Additionally, the effect 
      of LH on P4 secretion from CL slices collected from synchronized and naturally 
      ovulated animals has been studied in vitro. PGF2alpha /eCG/hCG administration 
      increased mRNA expression of StAR, CYP11A1, 3betaHSD, and LHR on day 9 and CYP11A1 
      and LHR on day 12 of pregnancy compared with the control group (p < 0.05). 
      CYP11A1, 3betaHSD, LHR, ERalpha and ERbeta proteins were not affected by synchronization; 
      only StAR protein increased in hormonally treated animals (p = 0.017). The 
      concentration of P4 in luteal tissue was greater on day 9 (p < 0.01), but lower 
      on day 16 (p < 0.05) in gilts with hormonally induced oestrus compared with 
      control animals. Blood serum levels of P4 were lower in synchronized than control 
      gilts (p < 0.001). Synchronization did not affect LH-stimulated P4 secretion from 
      luteal slices; however, greater basal concentration of P4 in incubation medium 
      was detected for CLs collected from synchronized than control gilts (p < 0.05). 
      In conclusion, synchronization of oestrus with PGF2alpha/eCG/hCG protocol in gilts 
      did not impair the expression of luteal P4 synthesis system, although decreased 
      P4 concentration in the blood.
CI  - (c) 2014 Blackwell Verlag GmbH.
FAU - Szymanska, M
AU  - Szymanska M
AD  - Institute of Animal Reproduction and Food Research of the Polish Academy of 
      Sciences, Olsztyn, Poland.
FAU - Morawska-Pucinska, E
AU  - Morawska-Pucinska E
FAU - Krawczynski, K
AU  - Krawczynski K
FAU - Kiewisz, J
AU  - Kiewisz J
FAU - Ziecik, A J
AU  - Ziecik AJ
FAU - Blitek, A
AU  - Blitek A
LA  - eng
PT  - Journal Article
PT  - Randomized Controlled Trial
PT  - Research Support, Non-U.S. Gov't
DEP - 20141008
PL  - Germany
TA  - Reprod Domest Anim
JT  - Reproduction in domestic animals = Zuchthygiene
JID - 9015668
RN  - 0 (Chorionic Gonadotropin)
RN  - 0 (Estrogen Receptor alpha)
RN  - 0 (Estrogen Receptor beta)
RN  - 0 (Phosphoproteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Estrogen)
RN  - 0 (Receptors, LH)
RN  - 0 (steroidogenic acute regulatory protein)
RN  - 4G7DS2Q64Y (Progesterone)
RN  - B7IN85G1HY (Dinoprost)
RN  - EC 1.1.- (Hydroxysteroid Dehydrogenases)
RN  - EC 1.14.15.6 (Cholesterol Side-Chain Cleavage Enzyme)
SB  - IM
MH  - Animals
MH  - Cholesterol Side-Chain Cleavage Enzyme/genetics/metabolism
MH  - Chorionic Gonadotropin/administration & dosage/*pharmacology
MH  - Dinoprost/administration & dosage/*pharmacology
MH  - Estrogen Receptor alpha/genetics/metabolism
MH  - Estrogen Receptor beta/genetics/metabolism
MH  - Estrus Synchronization/*methods
MH  - Female
MH  - Gene Expression Regulation/drug effects
MH  - Hydroxysteroid Dehydrogenases/genetics/metabolism
MH  - Phosphoproteins/blood/genetics/metabolism
MH  - Pregnancy
MH  - Progesterone/metabolism
MH  - RNA, Messenger/genetics/metabolism
MH  - Real-Time Polymerase Chain Reaction
MH  - Receptors, Estrogen/genetics/metabolism
MH  - Receptors, LH/genetics/metabolism
MH  - Swine/*physiology
EDAT- 2014/10/09 06:00
MHDA- 2015/08/04 06:00
CRDT- 2014/10/09 06:00
PHST- 2014/04/07 00:00 [received]
PHST- 2014/08/31 00:00 [accepted]
PHST- 2014/10/09 06:00 [entrez]
PHST- 2014/10/09 06:00 [pubmed]
PHST- 2015/08/04 06:00 [medline]
AID - 10.1111/rda.12433 [doi]
PST - ppublish
SO  - Reprod Domest Anim. 2014 Dec;49(6):1034-42. doi: 10.1111/rda.12433. Epub 2014 Oct 
      8.