PMID- 25293755
OWN - NLM
STAT- MEDLINE
DCOM- 20150728
LR  - 20220331
IS  - 1532-298X (Electronic)
IS  - 1040-4651 (Print)
IS  - 1040-4651 (Linking)
VI  - 26
IP  - 10
DP  - 2014 Oct
TI  - Arabidopsis lipins, PDAT1 acyltransferase, and SDP1 triacylglycerol lipase 
      synergistically direct fatty acids toward beta-oxidation, thereby maintaining 
      membrane lipid homeostasis.
PG  - 4119-34
LID - 10.1105/tpc.114.130377 [doi]
AB  - Triacylglycerol (TAG) metabolism is a key aspect of intracellular lipid 
      homeostasis in yeast and mammals, but its role in vegetative tissues of plants 
      remains poorly defined. We previously reported that PHOSPHOLIPID:DIACYLGLYCEROL 
      ACYLTRANSFERASE1 (PDAT1) is crucial for diverting fatty acids (FAs) from membrane 
      lipid synthesis to TAG and thereby protecting against FA-induced cell death in 
      leaves. Here, we show that overexpression of PDAT1 enhances the turnover of FAs 
      in leaf lipids. Using the trigalactosyldiacylglycerol1-1 (tgd1-1) mutant, which 
      displays substantially enhanced PDAT1-mediated TAG synthesis, we demonstrate that 
      disruption of SUGAR-DEPENDENT1 (SDP1) TAG lipase or PEROXISOMAL TRANSPORTER1 
      (PXA1) severely decreases FA turnover, leading to increases in leaf TAG 
      accumulation, to 9% of dry weight, and in total leaf lipid, by 3-fold. The 
      membrane lipid composition of tgd1-1 sdp1-4 and tgd1-1 pxa1-2 double mutants is 
      altered, and their growth and development are compromised. We also show that two 
      Arabidopsis thaliana lipin homologs provide most of the diacylglycerol for TAG 
      synthesis and that loss of their functions markedly reduces TAG content, but with 
      only minor impact on eukaryotic galactolipid synthesis. Collectively, these 
      results show that Arabidopsis lipins, along with PDAT1 and SDP1, function 
      synergistically in directing FAs toward peroxisomal beta-oxidation via TAG 
      intermediates, thereby maintaining membrane lipid homeostasis in leaves.
CI  - (c) 2014 American Society of Plant Biologists. All rights reserved.
FAU - Fan, Jilian
AU  - Fan J
AD  - Bioscience Department, Brookhaven National Laboratory, Upton, New York 11973.
FAU - Yan, Chengshi
AU  - Yan C
AD  - Bioscience Department, Brookhaven National Laboratory, Upton, New York 11973.
FAU - Roston, Rebecca
AU  - Roston R
AD  - Department of Biochemistry and Molecular Biology, Michigan State University, East 
      Lansing, Michigan 48824.
FAU - Shanklin, John
AU  - Shanklin J
AD  - Bioscience Department, Brookhaven National Laboratory, Upton, New York 11973.
FAU - Xu, Changcheng
AU  - Xu C
AUID- ORCID: 0000-0002-0179-9462
AD  - Bioscience Department, Brookhaven National Laboratory, Upton, New York 11973 
      cxu@bnl.gov.
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20141007
PL  - England
TA  - Plant Cell
JT  - The Plant cell
JID - 9208688
RN  - 0 (ATP-Binding Cassette Transporters)
RN  - 0 (Arabidopsis Proteins)
RN  - 0 (Fatty Acids)
RN  - 0 (Membrane Lipids)
RN  - 0 (Membrane Transport Proteins)
RN  - 0 (TGD1 protein, Arabidopsis)
RN  - 0 (Triglycerides)
RN  - EC 2.3.- (Acyltransferases)
RN  - EC 2.3.1.- (PDAT1 protein, Arabidopsis)
RN  - EC 3.1.1.- (Carboxylic Ester Hydrolases)
RN  - EC 3.1.1.3 (Lipase)
RN  - EC 3.1.1.3 (SDP1 protein, Arabidopsis)
RN  - EC 3.2.1.21 (SFR2 protein, Arabidopsis)
RN  - EC 3.2.1.21 (beta-Glucosidase)
RN  - EC 3.6.1.- (Adenosine Triphosphatases)
RN  - EC 3.6.1.- (At4g39850 protein, Arabidopsis)
SB  - IM
CIN - Plant Cell. 2014 Oct;26(10):3827. PMID: 25351489
MH  - ATP-Binding Cassette Transporters/genetics/metabolism
MH  - Acyltransferases/genetics/*metabolism
MH  - Adenosine Triphosphatases
MH  - Arabidopsis/genetics/metabolism
MH  - Arabidopsis Proteins/genetics/*metabolism
MH  - Carboxylic Ester Hydrolases/genetics/*metabolism
MH  - Fatty Acids/*metabolism
MH  - Gene Expression Regulation, Plant
MH  - *Homeostasis
MH  - Lipase/genetics/metabolism
MH  - Lipid Droplets/metabolism/ultrastructure
MH  - Membrane Lipids/*metabolism
MH  - Membrane Transport Proteins/genetics/metabolism
MH  - Microscopy, Electron, Transmission
MH  - Microscopy, Fluorescence
MH  - Models, Biological
MH  - Mutation
MH  - Oxidation-Reduction
MH  - Peroxisomes/metabolism
MH  - Plant Leaves/genetics/metabolism/ultrastructure
MH  - Plants, Genetically Modified
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Triglycerides/metabolism
MH  - beta-Glucosidase/genetics/metabolism
PMC - PMC4247580
EDAT- 2014/10/09 06:00
MHDA- 2015/07/29 06:00
PMCR- 2015/10/01
CRDT- 2014/10/09 06:00
PHST- 2014/10/09 06:00 [entrez]
PHST- 2014/10/09 06:00 [pubmed]
PHST- 2015/07/29 06:00 [medline]
PHST- 2015/10/01 00:00 [pmc-release]
AID - tpc.114.130377 [pii]
AID - 130377 [pii]
AID - 10.1105/tpc.114.130377 [doi]
PST - ppublish
SO  - Plant Cell. 2014 Oct;26(10):4119-34. doi: 10.1105/tpc.114.130377. Epub 2014 Oct 
      7.