PMID- 25295749 OWN - NLM STAT- MEDLINE DCOM- 20150619 LR - 20141009 IS - 1532-2335 (Electronic) IS - 1525-7770 (Linking) VI - 33 IP - 11 DP - 2014 TI - DNA binding studies of hematoxylin-Dy(small sha, Cyrillic) complex by spectrometry using acridine orange as a probe. PG - 730-45 LID - 10.1080/15257770.2014.931589 [doi] AB - The interaction of a hematoxylin(HE)-Dy(capital SHA, Cyrillic) complex with herring sperm DNA(hsDNA) was studied using acridine orange(AO) as a probe by UV-vis absorption, circular dichroism(CD), fluorescence spectroscopy and viscosity measurements. From the results of the probe experiment, we found that the HE-Dy(capital SHA, Cyrillic) complex could compete with AO for intercalating into hsDNA. The binding constants of the HE-Dy(capital SHA, Cyrillic) complex to hsDNA was obtained by the double reciprocal method and indicated that the affinity between hsDNA and the complex is weaker than that between hsDNA and classical intercalators. The thermodynamic parameters(DeltaH degrees , DeltaG degrees , DeltaS degrees ) were calculated from the UV-vis absorption data measured at two different temperatures. Further experimental results suggested that there exist groove binding and partial intercalation binding between hsDNA and HE-Dy(capital SHA, Cyrillic) complex. FAU - Xiong, Xiaoli AU - Xiong X AD - a College of Chemistry and Materials Science , Sichuan Normal University , Chengdu , P.R.China. FAU - Huang, Jianhang AU - Huang J FAU - Wang, Xingming AU - Wang X LA - eng PT - Journal Article PL - United States TA - Nucleosides Nucleotides Nucleic Acids JT - Nucleosides, nucleotides & nucleic acids JID - 100892832 RN - 0 (Coordination Complexes) RN - 0 (Intercalating Agents) RN - 1D4N45714Q (Dysprosium) RN - 9007-49-2 (DNA) RN - F30N4O6XVV (Acridine Orange) RN - YKM8PY2Z55 (Hematoxylin) SB - IM MH - Acridine Orange MH - Animals MH - Circular Dichroism MH - Coordination Complexes/chemistry MH - DNA/*chemistry MH - Dysprosium/*chemistry MH - Fishes MH - Hematoxylin/*chemistry MH - Hydrophobic and Hydrophilic Interactions MH - Intercalating Agents/chemistry MH - Male MH - Spectrometry, Fluorescence MH - Spectrum Analysis MH - Spermatozoa/chemistry MH - Thermodynamics MH - Viscosity OTO - NOTNLM OT - DNA OT - Dy(III) OT - acridine orange OT - hematoxylin OT - spectrometry EDAT- 2014/10/09 06:00 MHDA- 2015/06/20 06:00 CRDT- 2014/10/09 06:00 PHST- 2014/10/09 06:00 [entrez] PHST- 2014/10/09 06:00 [pubmed] PHST- 2015/06/20 06:00 [medline] AID - 10.1080/15257770.2014.931589 [doi] PST - ppublish SO - Nucleosides Nucleotides Nucleic Acids. 2014;33(11):730-45. doi: 10.1080/15257770.2014.931589.