PMID- 25298580
OWN - NLM
STAT- MEDLINE
DCOM- 20150611
LR  - 20211021
IS  - 1998-3751 (Electronic)
IS  - 0253-7613 (Print)
IS  - 0253-7613 (Linking)
VI  - 46
IP  - 5
DP  - 2014 Sep-Oct
TI  - Effects of tongxinluo on the neointima formation and expression of inflammatory 
      cytokines in rats after carotid artery balloon injury.
PG  - 510-4
LID - 10.4103/0253-7613.140582 [doi]
AB  - OBJECTIVE: Tongxinluo (TXL) is a traditional Chinese medicine (TCM). It is used 
      to treat coronary heart disease and atherosclerosis. We investigated the effects 
      of TXL on the neointima formation and expression of inflammatory cytokines in 
      rats after carotid artery balloon injury. MATERIALS AND METHODS: Male 
      Sprague-Dawley rats were randomly divided into four groups: sham operation group 
      (Sham, n = 15), balloon injury group treated with vehicle (Control, n = 15), TXL 
      low-dose group treated with TXL of 0.5 g/kg/d (TXL-L, n = 15), and TXL high-dose 
      group treated with TXL of 1.0 g/kg/d (TXL-H, n = 15). TXL was given by gavage 
      daily. 14 days after injury', the levels of serum nitric oxide (NO), endothelin-1 
      (ET-1), monocyte chemoattractant protein-1 (MCP-1), and soluble intercellular 
      adhesion molecule-1 (sICAM-1) were evaluated. The morphology of carotid artery 
      tissue was observed with hematoxylin-eosin staining. Expressions of MCP-1 and 
      ICAM-1 in the artery were detected by real-time polymerase chain reaction 
      (RT-PCR) and western blotting. RESULTS: 14 days after injury, a significant 
      increase in concentrations of serum ET-1, MCP-1, and sICAM-1 (P < 0.05), as well 
      as a significant decrease in NO serum level were observed in rats subjected to 
      artery injury compared to the sham rats (P < 0.05). TXL significantly decreased 
      ET-1, MCP-1 and sICAM-1 serum levels (P < 0.05), whereas significantly increased 
      NO serum level compared with the control (P < 0.05). TXL significantly reduced 
      the neointimal thickening at day14 after injury (P < 0.05). In addition, TXL 
      significantly reduced mRNA and protein expressions of ICAM-1 and MCP-1 in injured 
      artery (P < 0.05). CONCLUSIONS: This study demonstrates that TXL is effective in 
      improving endothelial function, attenuating neointimal formation of artery after 
      balloon injury, and reducing expression of inflammatory cytokine MCP-1 and 
      ICAM-1. It may be a useful agent for protecting the artery against injury.
FAU - Yao, En-Hui
AU  - Yao EH
AD  - Department of Cardiology, Union Hospital of Fujian Medical University, Fujian 
      Institute of Coronary Artery Disease, Fuzhou, China.
FAU - Wang, Hua-Jun
AU  - Wang HJ
AD  - Fujian Hypertension Research Institute, the First Affiliated Hospital of Fujian 
      Medical University, Fuzhou, China.
FAU - Xu, Chang-Sheng
AU  - Xu CS
AD  - Fujian Hypertension Research Institute, the First Affiliated Hospital of Fujian 
      Medical University, Fuzhou, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - India
TA  - Indian J Pharmacol
JT  - Indian journal of pharmacology
JID - 7902477
RN  - 0 (Ccl2 protein, rat)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Cytokines)
RN  - 0 (Drugs, Chinese Herbal)
RN  - 0 (Inflammation Mediators)
RN  - 0 (RNA, Messenger)
RN  - 0 (tongxinluo)
RN  - 126547-89-5 (Intercellular Adhesion Molecule-1)
SB  - IM
MH  - Animals
MH  - Blotting, Western
MH  - Carotid Artery Injuries/*drug therapy/pathology
MH  - Chemokine CCL2/blood/drug effects/genetics
MH  - Cytokines/drug effects/genetics/metabolism
MH  - Disease Models, Animal
MH  - Dose-Response Relationship, Drug
MH  - Drugs, Chinese Herbal/administration & dosage/*pharmacology
MH  - Endothelium, Vascular/drug effects/pathology
MH  - Gene Expression Regulation/drug effects
MH  - Inflammation Mediators/metabolism
MH  - Intercellular Adhesion Molecule-1/blood/drug effects/genetics
MH  - Male
MH  - Neointima/*prevention & control
MH  - RNA, Messenger
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Real-Time Polymerase Chain Reaction
PMC - PMC4175887
OTO - NOTNLM
OT  - Carotid artery injury
OT  - inflammatory cytokine
OT  - neointima formation
OT  - tongxinluo
COIS- Conflict of Interest: No
EDAT- 2014/10/10 06:00
MHDA- 2015/06/13 06:00
PMCR- 2014/09/01
CRDT- 2014/10/10 06:00
PHST- 2013/12/15 00:00 [received]
PHST- 2014/03/15 00:00 [revised]
PHST- 2014/07/26 00:00 [accepted]
PHST- 2014/10/10 06:00 [entrez]
PHST- 2014/10/10 06:00 [pubmed]
PHST- 2015/06/13 06:00 [medline]
PHST- 2014/09/01 00:00 [pmc-release]
AID - IJPharm-46-510 [pii]
AID - 10.4103/0253-7613.140582 [doi]
PST - ppublish
SO  - Indian J Pharmacol. 2014 Sep-Oct;46(5):510-4. doi: 10.4103/0253-7613.140582.