PMID- 25298750
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20141009
LR  - 20211021
IS  - 1475-2867 (Print)
IS  - 1475-2867 (Electronic)
IS  - 1475-2867 (Linking)
VI  - 14
IP  - 1
DP  - 2014
TI  - Human umbilical cord mesenchymal stem cells promote carcinoma growth and lymph 
      node metastasis when co-injected with esophageal carcinoma cells in nude mice.
PG  - 93
LID - 10.1186/s12935-014-0093-9 [doi]
LID - 93
AB  - BACKGROUND: Human umbilical cord blood derived-mesenchymal stem cells (hUCMSCs) 
      offer an attractive alternative to bone marrow-derived MSCs (BMMSCs) for 
      cell-based therapy as it is a less invasive source of biological material. 
      However, limited studies have been conducted with hUCMSCs as compared to BMMSCs. 
      The present study was conducted to evaluate the effects of hUCMSCs in esophageal 
      carcinoma (EC). METHODS: hUCMSCs together with EC cells were transplanted 
      subcutaneously into BALB/c nude mice to observe the effects of hUCMSCs on tumor 
      establishment. hUCMSCs injected through the caudal vein to the mice with 
      pre-established EC to observe the effects of hUCMSCs on tumor outgrowth. In order 
      to elucidate the underlying mechanisms, we also performed in vitro experiments 
      including directly co-culture, transwell assay, proliferation assay and western 
      blotting analysis. RESULTS: hUCMSCs promoted EC formation in nude mice. In the in 
      vivo model of pre-established EC, intravenously injected hUCMSCs potently 
      promoted tumor growth. When in vitro co-cultured with hUCMSCs, EC cells 
      proliferation increased. After co-cultured with hUCMSCs through transwell system, 
      EC cells showed increased proliferation. Through transwell assay, we also 
      observed that EC cells recruited MSCs, and MSCs promoted EC cells migration and 
      invasion. Western blotting data showed that the expressions of proliferation 
      related proteins Bcl-2, survivin and metastasis related proteins MMP-2 and MMP-9 
      were up-regulated in the EC cells transwell co-cultured with hUCMSCs. 
      CONCLUSIONS: Our results indicated that hUCMSCs could favor tumor growth in vivo 
      and in vitro. Thus, the exploitation of hUCMSCs in new therapeutic strategies 
      should be cautious under the malignant conditions.
FAU - Yang, Xiaoya
AU  - Yang X
AD  - Blood Transfusion Department, The Bethune International Peace Hospital, 
      Shijiazhuang, 050082 Hebei P R. China.
FAU - Li, Zhu
AU  - Li Z
AD  - Blood Transfusion Department, The Bethune International Peace Hospital, 
      Shijiazhuang, 050082 Hebei P R. China.
FAU - Ma, Yintu
AU  - Ma Y
AD  - Blood Transfusion Department, The Bethune International Peace Hospital, 
      Shijiazhuang, 050082 Hebei P R. China.
FAU - Gao, Jun
AU  - Gao J
AD  - Blood Transfusion Department, The Bethune International Peace Hospital, 
      Shijiazhuang, 050082 Hebei P R. China.
FAU - Liu, Surui
AU  - Liu S
AD  - Blood Transfusion Department, The Bethune International Peace Hospital, 
      Shijiazhuang, 050082 Hebei P R. China.
FAU - Gao, Yuhua
AU  - Gao Y
AD  - Blood Transfusion Department, The Bethune International Peace Hospital, 
      Shijiazhuang, 050082 Hebei P R. China.
FAU - Wang, Gengyin
AU  - Wang G
AD  - Blood Transfusion Department, The Bethune International Peace Hospital, 
      Shijiazhuang, 050082 Hebei P R. China.
LA  - eng
PT  - Journal Article
DEP - 20140924
PL  - England
TA  - Cancer Cell Int
JT  - Cancer cell international
JID - 101139795
PMC - PMC4189553
OTO - NOTNLM
OT  - Esophageal carcinoma
OT  - Mesenchymal stem cells
OT  - Metastasis
OT  - Tumor growth
OT  - Umbilical cord
EDAT- 2014/10/10 06:00
MHDA- 2014/10/10 06:01
PMCR- 2014/09/24
CRDT- 2014/10/10 06:00
PHST- 2014/01/23 00:00 [received]
PHST- 2014/09/05 00:00 [accepted]
PHST- 2014/10/10 06:00 [entrez]
PHST- 2014/10/10 06:00 [pubmed]
PHST- 2014/10/10 06:01 [medline]
PHST- 2014/09/24 00:00 [pmc-release]
AID - 93 [pii]
AID - 10.1186/s12935-014-0093-9 [doi]
PST - epublish
SO  - Cancer Cell Int. 2014 Sep 24;14(1):93. doi: 10.1186/s12935-014-0093-9. 
      eCollection 2014.