PMID- 25298860
OWN - NLM
STAT- MEDLINE
DCOM- 20150610
LR  - 20211021
IS  - 1942-0994 (Electronic)
IS  - 1942-0900 (Print)
IS  - 1942-0994 (Linking)
VI  - 2014
DP  - 2014
TI  - Dual stimulus-dependent effect of Oenothera paradoxa extract on the respiratory 
      burst in human leukocytes: suppressing for Escherichia coli and phorbol myristate 
      acetate and stimulating for formyl-methionyl-leucyl-phenylalanine.
PG  - 764367
LID - 10.1155/2014/764367 [doi]
LID - 764367
AB  - Although a growing body of evidence suggests that plant polyphenols can modulate 
      human immune responses, their simultaneous action on monocyte and neutrophil 
      oxidative burst is currently poorly understood. Based on the hypothesis that 
      various polyphenols contained in plant extracts might affect the oxidative burst 
      of phagocytes, we evaluated the effects of ethanolic O. paradoxa extract 
      polyphenols on monocyte and neutrophil oxidative burst in vitro activated by 
      different stimuli, including opsonized bacteria E. coli, phorbol 12-myristate 
      13-acetate (PMA), and formyl-methionyl-leucyl-phenylalanine (fMLP). Samples were 
      analyzed by the dihydrorhodamine flow cytometry assay. Our results showed that 
      the extract repressed significantly and dose-dependently reactive oxygen species 
      production in both cell types stimulated with E. coli and PMA (P < 0.05) and its 
      inhibitory efficiency was stimulus- and cell-type-dependent. Interestingly, there 
      was significant stimulatory effect of the extract on bursting phagocytes induced 
      by fMLP (P < 0.05). Additionally, several flavonoids and phenolic compounds as 
      well as penta-galloyl-beta-(D)-glucose (PGG), the representative of hydrolyzable 
      tannins, were identified in the 60% extract by high-performance liquid 
      chromatography (HPLC) coupled to electrospray ionization in negative ion mode. In 
      summary, the ethanolic O. paradoxa extract, rich in flavonoids and phenolic 
      compounds, exhibits dual stimulus-dependent effect on the respiratory burst in 
      human leukocytes; hence, it might affect immune responses in humans.
FAU - Burzynska-Pedziwiatr, Izabela
AU  - Burzynska-Pedziwiatr I
AD  - Department of Structural Biology, Faculty of Biomedical Sciences and Postgraduate 
      Education, Medical University of Lodz, Zeligowskiego 7/9, 90-752 Lodz, Poland.
FAU - Bukowiecka-Matusiak, Malgorzata
AU  - Bukowiecka-Matusiak M
AD  - Department of Structural Biology, Faculty of Biomedical Sciences and Postgraduate 
      Education, Medical University of Lodz, Zeligowskiego 7/9, 90-752 Lodz, Poland.
FAU - Wojcik, Marzena
AU  - Wojcik M
AD  - Department of Structural Biology, Faculty of Biomedical Sciences and Postgraduate 
      Education, Medical University of Lodz, Zeligowskiego 7/9, 90-752 Lodz, Poland.
FAU - Machala, Waldemar
AU  - Machala W
AD  - Department of Anesthesiology and Intensive Therapy, Medical University of Lodz, 
      Zeromskiego 113, 90-710 Lodz, Poland.
FAU - Bienkiewicz, Malgorzata
AU  - Bienkiewicz M
AD  - Department of Quality Control and Radiological Protection, Medical University of 
      Lodz, Czechoslowacka 8/10, 92-216 Lodz, Poland.
FAU - Spolnik, Grzegorz
AU  - Spolnik G
AD  - Institute of Organic Chemistry, Polish Academy of Sciences, Kasprzaka 44/52, 
      01-224 Warsaw, Poland.
FAU - Danikiewicz, Witold
AU  - Danikiewicz W
AD  - Institute of Organic Chemistry, Polish Academy of Sciences, Kasprzaka 44/52, 
      01-224 Warsaw, Poland.
FAU - Wozniak, Lucyna Alicja
AU  - Wozniak LA
AD  - Department of Structural Biology, Faculty of Biomedical Sciences and Postgraduate 
      Education, Medical University of Lodz, Zeligowskiego 7/9, 90-752 Lodz, Poland.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140914
PL  - United States
TA  - Oxid Med Cell Longev
JT  - Oxidative medicine and cellular longevity
JID - 101479826
RN  - 0 (Plant Extracts)
RN  - 0 (Polyphenols)
RN  - 0 (Reactive Oxygen Species)
RN  - 59880-97-6 (N-Formylmethionine Leucyl-Phenylalanine)
RN  - 59881-08-2 (methionyl-leucyl-phenylalanine)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
SB  - IM
MH  - Chromatography, High Pressure Liquid
MH  - Drug Synergism
MH  - Escherichia coli/*physiology
MH  - Humans
MH  - Leukocytes/*drug effects/metabolism
MH  - N-Formylmethionine Leucyl-Phenylalanine/*analogs & derivatives/pharmacology
MH  - Oenothera/*chemistry
MH  - Plant Extracts/chemistry/*pharmacology
MH  - Polyphenols/chemistry/pharmacology
MH  - Reactive Oxygen Species/metabolism
MH  - Respiratory Burst/*drug effects
MH  - Seeds/chemistry
MH  - Tandem Mass Spectrometry
MH  - Tetradecanoylphorbol Acetate/*pharmacology
PMC - PMC4178919
EDAT- 2014/10/10 06:00
MHDA- 2015/06/11 06:00
PMCR- 2014/09/14
CRDT- 2014/10/10 06:00
PHST- 2014/05/06 00:00 [received]
PHST- 2014/08/15 00:00 [accepted]
PHST- 2014/10/10 06:00 [entrez]
PHST- 2014/10/10 06:00 [pubmed]
PHST- 2015/06/11 06:00 [medline]
PHST- 2014/09/14 00:00 [pmc-release]
AID - 10.1155/2014/764367 [doi]
PST - ppublish
SO  - Oxid Med Cell Longev. 2014;2014:764367. doi: 10.1155/2014/764367. Epub 2014 Sep 
      14.