PMID- 25299114
OWN - NLM
STAT- MEDLINE
DCOM- 20150625
LR  - 20220330
IS  - 1676-5680 (Electronic)
IS  - 1676-5680 (Linking)
VI  - 13
IP  - 3
DP  - 2014 Sep 29
TI  - Study of human acellular amniotic membrane loading bone marrow mesenchymal stem 
      cells in repair of articular cartilage defect in rabbits.
PG  - 7992-8001
LID - 10.4238/2014.September.29.12 [doi]
AB  - The aim of this study was to investigate the repair effect of human acellular 
      amniotic membrane (HAAM) loading bone marrow mesenchymal stem cells (BMSCs) on 
      articular cartilage defect in rabbits. Rabbit BMSCs were isolated and cultured, 
      and they were then inoculated on HAAM to prepare the complex of HAAM and BMSCs. 
      Twenty-four rabbits were randomly divided into groups A and B, with 12 animals in 
      each group. The left and right sides were used as the experimental and control 
      sides, respectively. The models of bilateral articular cartilage defect were 
      established. The defect areas on the experimental side in groups A and B were 
      implanted with the complex of HAAM and BMSCs and HAAM alone, respectively. The 
      control sides of the two groups were not implanted with any material. In the 8th 
      and 12th week after surgery, gross observation, histological examination and 
      cartilage defect scoring were performed. In the 8th and 12th postoperative week, 
      gross observation and histological observation showed that dense cartilage-like 
      cells appeared in group A but not in group B, indicating preferable cartilage 
      repair. The cartilage defect score on the experimental side in group A was 5.31 +/- 
      0.68 in the 8th week and 3.23 +/- 0.52 in the 12th week, and that in group A was 
      significantly lower than in group B (P < 0.05). HAAM loading BMSCs has a good 
      repair effect on articular cartilage defect under an in vitro environment.
FAU - Liu, P-F
AU  - Liu PF
AD  - Department of Orthopaedics, Affiliated Zhongshan Hospital of Dalian University, 
      Dalian, China.
FAU - Guo, L
AU  - Guo L
AD  - Department of Orthopaedics, Affiliated Zhongshan Hospital of Dalian University, 
      Dalian, China linguodoc@163.com.
FAU - Zhao, D-W
AU  - Zhao DW
AD  - Department of Orthopaedics, Affiliated Zhongshan Hospital of Dalian University, 
      Dalian, China.
FAU - Zhang, Z-J
AU  - Zhang ZJ
AD  - Department of Orthopaedics, Affiliated Zhongshan Hospital of Dalian University, 
      Dalian, China.
FAU - Kang, K
AU  - Kang K
AD  - Department of Orthopaedics, Affiliated Zhongshan Hospital of Dalian University, 
      Dalian, China.
FAU - Zhu, R-P
AU  - Zhu RP
AD  - Department of Orthopaedics, Affiliated Zhongshan Hospital of Dalian University, 
      Dalian, China.
FAU - Yuan, X-L
AU  - Yuan XL
AD  - Department of Orthopaedics, Affiliated Zhongshan Hospital of Dalian University, 
      Dalian, China.
LA  - eng
PT  - Journal Article
DEP - 20140929
PL  - Brazil
TA  - Genet Mol Res
JT  - Genetics and molecular research : GMR
JID - 101169387
SB  - IM
MH  - *Amnion
MH  - Animals
MH  - Bone Marrow Cells/*cytology
MH  - Cartilage, Articular/*pathology
MH  - Humans
MH  - Knee Joint/*pathology
MH  - Mesenchymal Stem Cells/*cytology
MH  - Rabbits
EDAT- 2014/10/10 06:00
MHDA- 2015/06/26 06:00
CRDT- 2014/10/10 06:00
PHST- 2014/10/10 06:00 [entrez]
PHST- 2014/10/10 06:00 [pubmed]
PHST- 2015/06/26 06:00 [medline]
AID - gmr3967 [pii]
AID - 10.4238/2014.September.29.12 [doi]
PST - epublish
SO  - Genet Mol Res. 2014 Sep 29;13(3):7992-8001. doi: 10.4238/2014.September.29.12.