PMID- 25301281
OWN - NLM
STAT- MEDLINE
DCOM- 20150608
LR  - 20211021
IS  - 1873-2542 (Electronic)
IS  - 0378-1135 (Print)
IS  - 0378-1135 (Linking)
VI  - 174
IP  - 1-2
DP  - 2014 Nov 7
TI  - Lung pathogenicity of European genotype 3 strain porcine reproductive and 
      respiratory syndrome virus (PRRSV) differs from that of subtype 1 strains.
PG  - 127-38
LID - S0378-1135(14)00433-7 [pii]
LID - 10.1016/j.vetmic.2014.09.010 [doi]
AB  - Porcine reproductive and respiratory syndrome (PRRS) is difficult to control due 
      to a high mutation rate of the PRRS virus (PRRSV) and the emergence of virulent 
      strains. The objective of this study was to analyse early and late pathological 
      responses in the respiratory tract after infection with the European PRRSV 
      subtype 3 strain Lena in comparison to two European PRRSV subtype 1 strains: 
      Belgium A and Lelystad-Ter Huurne (LV). For each virus strain, groups of twelve 
      pigs were inoculated, and four pigs per group were euthanized at days 3, 7 and 35 
      post-infection (p.i.) for consecutive examination. Infection with strain Lena 
      resulted in a more severe disease than with the subtype 1 strains, an 
      inflammatory response within the first week of infection with expression of IL-1alpha 
      in the lung and lymph node, and an influx of neutrophils and monocytes in 
      bronchoalveolar lavage fluid (BALF). Infection with strain Belgium A or LV 
      resulted in mild or no pathology within the first week of infection, but 
      inflammatory cell influx in the lung interstititium was increased at the end of 
      the experiment at day 35 p.i. At five weeks p.i., all strains induced a higher 
      percentage of cytotoxic T cells and higher levels of IFN-gamma producing cells in 
      BALF. This might have contributed to clearance of virus. In general, subtype 3 
      strain Lena induced a stronger early inflammatory response which led to more 
      severe clinical disease and pathology. On the other hand, this may have supported 
      an enhanced or faster clearance of virus in tissues, compared to subtype 1 
      strains.
CI  - Copyright (c) 2014 Elsevier B.V. All rights reserved.
FAU - Weesendorp, Eefke
AU  - Weesendorp E
AD  - Department of Infection Biology, Central Veterinary Institute, Part of Wageningen 
      UR, PO Box 65, 8200 AB Lelystad, The Netherlands. Electronic address: 
      eefke.weesendorp@wur.nl.
FAU - Rebel, Johanna M J
AU  - Rebel JM
AD  - Department of Infection Biology, Central Veterinary Institute, Part of Wageningen 
      UR, PO Box 65, 8200 AB Lelystad, The Netherlands.
FAU - Popma-De Graaf, Ditta J
AU  - Popma-De Graaf DJ
AD  - Department of Infection Biology, Central Veterinary Institute, Part of Wageningen 
      UR, PO Box 65, 8200 AB Lelystad, The Netherlands.
FAU - Fijten, Helmi P D
AU  - Fijten HP
AD  - Department of Infection Biology, Central Veterinary Institute, Part of Wageningen 
      UR, PO Box 65, 8200 AB Lelystad, The Netherlands.
FAU - Stockhofe-Zurwieden, Norbert
AU  - Stockhofe-Zurwieden N
AD  - Department of Infection Biology, Central Veterinary Institute, Part of Wageningen 
      UR, PO Box 65, 8200 AB Lelystad, The Netherlands.
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140922
PL  - Netherlands
TA  - Vet Microbiol
JT  - Veterinary microbiology
JID - 7705469
SB  - IM
MH  - Animals
MH  - Belgium
MH  - Body Temperature
MH  - Bronchoalveolar Lavage Fluid/virology
MH  - Flow Cytometry/veterinary
MH  - Genotype
MH  - Immunohistochemistry/veterinary
MH  - Immunophenotyping/veterinary
MH  - Inflammation/*pathology
MH  - Lung/pathology/*virology
MH  - Monocytes/immunology
MH  - Porcine Reproductive and Respiratory Syndrome/*immunology/*pathology
MH  - Porcine respiratory and reproductive syndrome virus/genetics/*pathogenicity
MH  - Reverse Transcriptase Polymerase Chain Reaction/veterinary
MH  - Species Specificity
MH  - Statistics, Nonparametric
MH  - Swine
MH  - Viremia/veterinary
MH  - Virulence
PMC - PMC7117439
OTO - NOTNLM
OT  - Genetic subtypes
OT  - Immune response
OT  - Pathogenesis
OT  - Porcine reproductive and respiratory syndrome virus
EDAT- 2014/10/11 06:00
MHDA- 2015/06/09 06:00
PMCR- 2014/09/22
CRDT- 2014/10/11 06:00
PHST- 2014/07/29 00:00 [received]
PHST- 2014/09/08 00:00 [revised]
PHST- 2014/09/10 00:00 [accepted]
PHST- 2014/10/11 06:00 [entrez]
PHST- 2014/10/11 06:00 [pubmed]
PHST- 2015/06/09 06:00 [medline]
PHST- 2014/09/22 00:00 [pmc-release]
AID - S0378-1135(14)00433-7 [pii]
AID - 10.1016/j.vetmic.2014.09.010 [doi]
PST - ppublish
SO  - Vet Microbiol. 2014 Nov 7;174(1-2):127-38. doi: 10.1016/j.vetmic.2014.09.010. 
      Epub 2014 Sep 22.