PMID- 25301672
OWN - NLM
STAT- MEDLINE
DCOM- 20150818
LR  - 20221207
IS  - 1865-3774 (Electronic)
IS  - 0925-5710 (Linking)
VI  - 100
IP  - 6
DP  - 2014 Dec
TI  - CpG Oligonucleotide and Interleukin 2 stimulation enables higher cytogenetic 
      abnormality detection rates than 12-o-tetradecanolyphorbol-13-acetate in Asian 
      patients with B-cell chronic lymphocytic leukemia (B-CLL).
PG  - 545-53
LID - 10.1007/s12185-014-1681-0 [doi]
AB  - The present study was designed to compare abnormality detection rates using DSP30 
      + IL2 and 12-O-Tetradecanoylphorbol-13-acetate (TPA) in Asian patients with 
      B-CLL. Hematological specimens from 47 patients (29 newly diagnosed, 18 relapsed) 
      were established as 72 h-DSP30 + IL2 and TPA cultures. Standard methods were 
      employed to identify clonal aberrations by conventional cytogenetics (CC). The 
      B-CLL fluorescence in situ hybridization (FISH) panel comprised ATM, CEP12, 
      D13S25, and TP53 probes. DSP30 + IL2 cultures had a higher chromosomal 
      abnormality detection rate (67 %) compared to TPA (44 %, p < 0.001). The mean 
      number of analyzable metaphases and abnormal metaphases per slide was also higher 
      (p < 0.005, p < 0.001, respectively). Culture success rate, percentage of complex 
      karyotype, and percentage of non-clonal abnormal cell were not significantly 
      different (p > 0.05). Thirteen cases with abnormalities were found exclusively in 
      DSP30 + IL2 cultures compared to one found solely in TPA cultures. DSP30 + IL2 
      cultures were comparable to the FISH panel in detecting 11q-, +12 and 17p- but 
      not 13q-. It also has a predilection for 11q- bearing leukemic cells compared to 
      TPA. FISH had a higher abnormality detection rate (84.1 %) compared to CC (66.0 
      %) with borderline significance (p = 0.051), albeit limited by its coverage. In 
      conclusion, DSP30 + IL2 showed a higher abnormality detection rate. However, FISH 
      is indispensable to circumvent low mitotic indices and detect subtle 
      abnormalities.
FAU - Liaw, Fiona Pui San
AU  - Liaw FP
AD  - Cytogenetics Laboratory, Department of Pathology, Singapore General Hospital, 
      Academia, Level 9, Diagnostics Tower, 20 College Road, Singapore, 169856, 
      Singapore, fionaliaw@hotmail.com.
FAU - Lau, Lai Ching
AU  - Lau LC
FAU - Lim, Alvin Soon Tiong
AU  - Lim AS
FAU - Lim, Tse Hui
AU  - Lim TH
FAU - Lee, Geok Yee
AU  - Lee GY
FAU - Tien, Sim Leng
AU  - Tien SL
LA  - eng
PT  - Journal Article
DEP - 20141010
PL  - Japan
TA  - Int J Hematol
JT  - International journal of hematology
JID - 9111627
RN  - 0 (CPG-oligonucleotide)
RN  - 0 (Interleukin-2)
RN  - 0 (Oligodeoxyribonucleotides)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
SB  - IM
MH  - Aged
MH  - *Asian People
MH  - *Chromosome Aberrations
MH  - Cytogenetics/methods/standards
MH  - Female
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence/methods/standards
MH  - Interleukin-2/pharmacology
MH  - Leukemia, Lymphocytic, Chronic, B-Cell/*diagnosis/*genetics
MH  - Male
MH  - Middle Aged
MH  - Oligodeoxyribonucleotides/pharmacology
MH  - Sensitivity and Specificity
MH  - Tetradecanoylphorbol Acetate/pharmacology
EDAT- 2014/10/11 06:00
MHDA- 2015/08/19 06:00
CRDT- 2014/10/11 06:00
PHST- 2014/05/21 00:00 [received]
PHST- 2014/09/21 00:00 [accepted]
PHST- 2014/09/19 00:00 [revised]
PHST- 2014/10/11 06:00 [entrez]
PHST- 2014/10/11 06:00 [pubmed]
PHST- 2015/08/19 06:00 [medline]
AID - 10.1007/s12185-014-1681-0 [doi]
PST - ppublish
SO  - Int J Hematol. 2014 Dec;100(6):545-53. doi: 10.1007/s12185-014-1681-0. Epub 2014 
      Oct 10.