PMID- 25302342
OWN - NLM
STAT- MEDLINE
DCOM- 20151022
LR  - 20141028
IS  - 1364-5528 (Electronic)
IS  - 0003-2654 (Linking)
VI  - 139
IP  - 23
DP  - 2014 Dec 7
TI  - An accurate and transferable protocol for reproducible quantification of organic 
      pollutants in human serum using direct isotope dilution mass spectrometry.
PG  - 6223-31
LID - 10.1039/c4an00851k [doi]
AB  - A robust method has been developed for easy transfer between analytical 
      laboratories to obtain highly accurate and reproducible quantification of 
      persistent organic pollutants (POPs) in micro-volumes of serum. This method is 
      suited for analysts researching the impact of environmental exposure on human 
      health. When performed by highly trained analysts, existing methods can produce 
      high quality data; however, complex sample preparation steps often cannot be 
      consistently replicated by laboratories, leading to variance in extraction 
      recovery and quantitation. By combining stir-bar sorptive extraction (SBSE) with 
      direct isotope dilution (D-ID) mass spectrometry quantification, a new analytical 
      method was developed. The D-ID quantification significantly improved accuracy, 
      corrected sample-to-sample irreproducibility, and reduced sample preparation 
      time. Independent production of statistically identical data then confirmed 
      transfer of the validated operating protocol to an off-site laboratory with 
      different instrument models. SBSE performance was compared with industry-accepted 
      extraction techniques. D-ID quantification was compared with peer-reviewed 
      relative isotopic response factor (RF) quantification methods. Holding other 
      variables constant, D-ID improved accuracy by 250% and precision by 300% compared 
      with RF; SBSE improved accuracy by 37% compared to industry-accepted extraction 
      methods. Limits of quantification of the analytes ranged from 60 pg g(-1) to 1 mug 
      g(-1). Protocol transfer exhibited <7% mean between-laboratory error and <2% mean 
      within-laboratory RSD. These results indicate that a transferable method has been 
      developed for academic, government, commercial, and clinical laboratories seeking 
      to maximize throughput and improve quantitative validity. This validated method 
      was applied in a recent clinical study to assess non-communicable disease in 
      children in Pennsylvania, USA.
FAU - Boggess, Andrew J
AU  - Boggess AJ
AD  - Department of Chemistry and Biochemistry, Duquesne University, USA. 
      kingston@duq.edu.
FAU - Rahman, G M Mizanur
AU  - Rahman GM
FAU - Pamukcu, Matt
AU  - Pamukcu M
FAU - Faber, Scott
AU  - Faber S
FAU - Kingston, H M Skip
AU  - Kingston HM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Analyst
JT  - The Analyst
JID - 0372652
RN  - 0 (Environmental Pollutants)
RN  - 0 (Organic Chemicals)
SB  - IM
MH  - Blood Chemical Analysis/*methods
MH  - Environmental Pollutants/*blood/chemistry
MH  - Humans
MH  - Mass Spectrometry/*methods
MH  - Organic Chemicals/*chemistry
MH  - Reproducibility of Results
EDAT- 2014/10/11 06:00
MHDA- 2015/10/23 06:00
CRDT- 2014/10/11 06:00
PHST- 2014/10/11 06:00 [entrez]
PHST- 2014/10/11 06:00 [pubmed]
PHST- 2015/10/23 06:00 [medline]
AID - 10.1039/c4an00851k [doi]
PST - ppublish
SO  - Analyst. 2014 Dec 7;139(23):6223-31. doi: 10.1039/c4an00851k.