PMID- 25303285
OWN - NLM
STAT- MEDLINE
DCOM- 20150629
LR  - 20211021
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 9
IP  - 10
DP  - 2014
TI  - Persistence of asthmatic response after ammonium persulfate-induced occupational 
      asthma in mice.
PG  - e109000
LID - 10.1371/journal.pone.0109000 [doi]
LID - e109000
AB  - INTRODUCTION: Since persulfate salts are an important cause of occupational 
      asthma (OA), we aimed to study the persistence of respiratory symptoms after a 
      single exposure to ammonium persulfate (AP) in AP-sensitized mice. MATERIAL AND 
      METHODS: BALB/c mice received dermal applications of AP or dimethylsulfoxide 
      (DMSO) on days 1 and 8. On day 15, they received a single nasal instillation of 
      AP or saline. Airway hyperresponsiveness (AHR) was assessed using methacholine 
      provocation, while pulmonary inflammation was evaluated in bronchoalveolar lavage 
      (BAL), and total serum immunoglobulin E (IgE), IgG1 and IgG2a were measured in 
      blood at 1, 4, 8, 24 hours and 4, 8, 15 days after the single exposure to the 
      causal agent. Histological studies of lungs were assessed. RESULTS: AP-treated 
      mice showed a sustained increase in AHR, lasting up to 4 days after the 
      challenge. There was a significant increase in the percentage of neutrophils 8 
      hours after the challenge, which persisted for 24 hours in AP-treated mice. The 
      extent of airway inflammation was also seen in the histological analysis of the 
      lungs from challenged mice. Slight increases in total serum IgE 4 days after the 
      challenge were found, while IgG gradually increased further 4 to 15 days after 
      the AP challenge in AP-sensitized mice. CONCLUSIONS: In AP-sensitized mice, an 
      Ig-independent response is induced after AP challenge. AHR appears immediately, 
      but airway neutrophil inflammation appears later. This response decreases in 
      time; at early stages only respiratory and inflammatory responses decrease, but 
      later on immunological response decreases as well.
FAU - Olle-Monge, Marta
AU  - Olle-Monge M
AD  - Servicio de Neumologia, Hospital Universitario Vall d'Hebron, Barcelona, Spain; 
      CIBER Enfermedades Respiratorias (CibeRes), Barcelona, Spain; Departament de 
      Medicina, Universitat Autonoma de Barcelona, Barcelona, Spain.
FAU - Munoz, Xavier
AU  - Munoz X
AD  - Servicio de Neumologia, Hospital Universitario Vall d'Hebron, Barcelona, Spain; 
      CIBER Enfermedades Respiratorias (CibeRes), Barcelona, Spain; Department of Cell 
      Biology, Physiology and Immunology, Universitat Autonoma de Barcelona, Barcelona, 
      Spain.
FAU - Vanoirbeek, Jeroen A J
AU  - Vanoirbeek JA
AD  - Centre for Environment and Health, KU Leuven, Leuven, Belgium.
FAU - Gomez-Olles, Susana
AU  - Gomez-Olles S
AD  - Servicio de Neumologia, Hospital Universitario Vall d'Hebron, Barcelona, Spain; 
      CIBER Enfermedades Respiratorias (CibeRes), Barcelona, Spain.
FAU - Morell, Ferran
AU  - Morell F
AD  - Servicio de Neumologia, Hospital Universitario Vall d'Hebron, Barcelona, Spain; 
      CIBER Enfermedades Respiratorias (CibeRes), Barcelona, Spain.
FAU - Cruz, Maria-Jesus
AU  - Cruz MJ
AD  - Servicio de Neumologia, Hospital Universitario Vall d'Hebron, Barcelona, Spain; 
      CIBER Enfermedades Respiratorias (CibeRes), Barcelona, Spain.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20141010
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Immunoglobulin G)
RN  - 22QF6L357F (ammonium peroxydisulfate)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - SU46BAM238 (Ammonium Sulfate)
SB  - IM
MH  - *Ammonium Sulfate
MH  - Animals
MH  - Asthma, Occupational/blood/*chemically induced/immunology/pathology
MH  - Bronchoalveolar Lavage
MH  - Disease Models, Animal
MH  - Immunoglobulin E/blood/immunology
MH  - Immunoglobulin G/blood/immunology
MH  - Inflammation/blood/immunology/pathology
MH  - Lung/immunology/*pathology
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
PMC - PMC4193836
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2014/10/11 06:00
MHDA- 2015/06/30 06:00
PMCR- 2014/10/10
CRDT- 2014/10/11 06:00
PHST- 2014/06/04 00:00 [received]
PHST- 2014/08/31 00:00 [accepted]
PHST- 2014/10/11 06:00 [entrez]
PHST- 2014/10/11 06:00 [pubmed]
PHST- 2015/06/30 06:00 [medline]
PHST- 2014/10/10 00:00 [pmc-release]
AID - PONE-D-14-24354 [pii]
AID - 10.1371/journal.pone.0109000 [doi]
PST - epublish
SO  - PLoS One. 2014 Oct 10;9(10):e109000. doi: 10.1371/journal.pone.0109000. 
      eCollection 2014.