PMID- 25304199 OWN - NLM STAT- MEDLINE DCOM- 20150819 LR - 20150202 IS - 1872-7573 (Electronic) IS - 0378-8741 (Linking) VI - 158 Pt A DP - 2014 Dec 2 TI - Cecropia pachystachya extract attenuated the renal lesion in 5/6 nephrectomized rats by reducing inflammation and renal arginase activity. PG - 49-57 LID - S0378-8741(14)00697-7 [pii] LID - 10.1016/j.jep.2014.09.042 [doi] AB - ETHNOPHARMACOLOGICAL RELEVANCE: The plant Cecropia pachystachya Trecul has been used in Brazilian folk medicine to treat hypertension, bladder and kidney inflammation and renal diseases. The aim of this study was to evaluate the potential of the aqueous fraction from the ethanolic extract of Cecropia pachystachya (FCP) in the management of hypertension, inflammation and progressive renal disease in rats submitted to 5/6 nephrectomy. MATERIALS AND METHODS: Thirty male Wistar rats submitted to 5/6 nephrectomy (5/6 NE) were untreated (NE) or treated (NE+FCP) with the FCP (0.5g/kg/day). The treatment started 15 days after surgery, and the rats were followed for a period of 60 days. Systolic blood pressure (SBP) and albuminuria were evaluated from 15-60 days after the surgical procedure. Function and estructural renal changes, TGF-beta (transforming growth factor beta), MCP-1 (monocyte chemoattractant protein-1) and nitric oxide (NO) urinary excretion were analyzed. Expression and activity of the renal enzymes arginase (ARG), angiotensin converting enzyme (ACE), and MAP kinase p-JNK expression also were analyzed. RESULTS: The nephrectomized rats developed progressive albuminuria and increased SBP that was less intense in the treated group. There was a reduction in the glomerular filtration rate (GFR) in the nephrectomized rats, which was attenuated by treatment with FCP extract. The treatment with FCP also attenuated the histological changes, reduced the expression and activity of renal arginase, the number of macrophages (ED-1 positive cells) and the p-JNK expression in the renal cortex of the rats submitted to 5/6 NE. The urinary excretion of TGF-beta was less intense in the treated group and was associated with the reduction of the expression and activity of the renal arginase. CONCLUSIONS: These results suggest that the reduction of renal arginase activity, p-JNK and TGF-beta expression can explain the mechanism by which the treatment with C. pachystachya reduced the inflammation and improved renal function. This study presents the potential use of Cecropia pachystachya in the treatment of chronic renal diseases. CI - Copyright (c) 2014 Elsevier Ireland Ltd. All rights reserved. FAU - Maquiaveli, Claudia C AU - Maquiaveli CC AD - Departament of Physiology, School of Medicine of Ribeirao Preto, University of Sao Paulo, Av. Bandeirantes, 3900 Monte Alegre, Ribeirao Preto, Sao Paulo, Brazil. Electronic address: cmaquiaveli@usp.br. FAU - da Silva, Edson R AU - da Silva ER AD - Department of Veterinary Medicine, School of Animal Science and Food Engineering, University of Sao Paulo, Av. Duque de Caxias Norte, 225, Pirassununga, Sao Paulo, Brazil. Electronic address: edsilva@usp.br. FAU - Rosa, Lara C AU - Rosa LC AD - Department of Veterinary Medicine, School of Animal Science and Food Engineering, University of Sao Paulo, Av. Duque de Caxias Norte, 225, Pirassununga, Sao Paulo, Brazil. FAU - Francescato, Heloisa D C AU - Francescato HD AD - Departament of Physiology, School of Medicine of Ribeirao Preto, University of Sao Paulo, Av. Bandeirantes, 3900 Monte Alegre, Ribeirao Preto, Sao Paulo, Brazil. FAU - Lucon Junior, Joao Francisco AU - Lucon Junior JF AD - Department of Veterinary Medicine, School of Animal Science and Food Engineering, University of Sao Paulo, Av. Duque de Caxias Norte, 225, Pirassununga, Sao Paulo, Brazil. FAU - Silva, Cleonice G A AU - Silva CG AD - Departament of Physiology, School of Medicine of Ribeirao Preto, University of Sao Paulo, Av. Bandeirantes, 3900 Monte Alegre, Ribeirao Preto, Sao Paulo, Brazil. FAU - Casarini, Dulce E AU - Casarini DE AD - Department of Medicine, Nephrology Division, Federal University of Sao Paulo, Rua Botucatu, 740, Vila Clementino, Sao Paulo, Brazil. FAU - Ronchi, Fernanda A AU - Ronchi FA AD - Department of Medicine, Nephrology Division, Federal University of Sao Paulo, Rua Botucatu, 740, Vila Clementino, Sao Paulo, Brazil. FAU - Coimbra, Terezila M AU - Coimbra TM AD - Departament of Physiology, School of Medicine of Ribeirao Preto, University of Sao Paulo, Av. Bandeirantes, 3900 Monte Alegre, Ribeirao Preto, Sao Paulo, Brazil. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20141007 PL - Ireland TA - J Ethnopharmacol JT - Journal of ethnopharmacology JID - 7903310 RN - 0 (Plant Extracts) RN - 0 (Transforming Growth Factor beta) RN - EC 2.7.11.24 (JNK Mitogen-Activated Protein Kinases) RN - EC 3.5.3.1 (Arginase) SB - IM MH - Albuminuria/drug therapy MH - Animals MH - Arginase/metabolism MH - Brazil MH - Cecropia Plant/*chemistry MH - Disease Progression MH - Glomerular Filtration Rate/drug effects MH - Hypertension/drug therapy MH - Inflammation/*drug therapy MH - JNK Mitogen-Activated Protein Kinases/genetics MH - Kidney/drug effects/enzymology/physiopathology MH - Kidney Diseases/*drug therapy/enzymology MH - Male MH - Medicine, Traditional MH - Nephrectomy MH - Plant Extracts/*pharmacology MH - Rats MH - Rats, Wistar MH - Transforming Growth Factor beta/genetics OTO - NOTNLM OT - Angiotensin II OT - Arginase OT - Cecropia pachystachya OT - Hypertension OT - Inflammation OT - Renal diseases EDAT- 2014/10/12 06:00 MHDA- 2015/08/20 06:00 CRDT- 2014/10/12 06:00 PHST- 2014/05/29 00:00 [received] PHST- 2014/09/19 00:00 [revised] PHST- 2014/09/28 00:00 [accepted] PHST- 2014/10/12 06:00 [entrez] PHST- 2014/10/12 06:00 [pubmed] PHST- 2015/08/20 06:00 [medline] AID - S0378-8741(14)00697-7 [pii] AID - 10.1016/j.jep.2014.09.042 [doi] PST - ppublish SO - J Ethnopharmacol. 2014 Dec 2;158 Pt A:49-57. doi: 10.1016/j.jep.2014.09.042. Epub 2014 Oct 7.